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Single-cell transcriptome sequencing reveals tumor heterogeneity in family neuroblastoma

Neuroblastoma(NB) is the most common extracranial solid tumor in childhood, and it is now believed that some patients with NB have an underlying genetic susceptibility, which may be one of the reasons for the multiplicity of NB patients within a family line. Even within the same family, the samples...

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Autores principales: Zhang, Yunlong, Ma, Yue, Liu, Qingqing, Du, Yifei, Peng, Liang, Zhou, Jianwu, Zhao, Zhenzhen, Li, Changchun, Wang, Shan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10543897/
https://www.ncbi.nlm.nih.gov/pubmed/37790931
http://dx.doi.org/10.3389/fimmu.2023.1197773
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author Zhang, Yunlong
Ma, Yue
Liu, Qingqing
Du, Yifei
Peng, Liang
Zhou, Jianwu
Zhao, Zhenzhen
Li, Changchun
Wang, Shan
author_facet Zhang, Yunlong
Ma, Yue
Liu, Qingqing
Du, Yifei
Peng, Liang
Zhou, Jianwu
Zhao, Zhenzhen
Li, Changchun
Wang, Shan
author_sort Zhang, Yunlong
collection PubMed
description Neuroblastoma(NB) is the most common extracranial solid tumor in childhood, and it is now believed that some patients with NB have an underlying genetic susceptibility, which may be one of the reasons for the multiplicity of NB patients within a family line. Even within the same family, the samples show great variation and can present as ganglioneuroblastoma or even benign ganglioneuroma. The genomics of NB is still unclear and more in-depth studies are needed to reveal its key components. We first performed single-cell RNA sequencing(sc-RNAseq) analysis on clinical specimens of two family neuroblastoma(FNB) and four sporadic NB cases. A complete transcriptional profile of FNB was constructed from 18,394 cells from FNB, and we found that SDHD may be genetically associated with FNB and identified a prognostic related CAF subtype in FNB: Fib-4. Single-cell flux estimation analysis (scFEA) results showed that malignant cells were associated with arginine spermine, oxaloacetate and hypoxanthine, and that malignant cells metabolize lactate at lower levels than T cells. Our study provides new resources and ideas for the development of the genomics of family NB, and the mechanisms of cell-to-cell interactions and communication and the metabolic landscape will provide new therapeutic targets.
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spelling pubmed-105438972023-10-03 Single-cell transcriptome sequencing reveals tumor heterogeneity in family neuroblastoma Zhang, Yunlong Ma, Yue Liu, Qingqing Du, Yifei Peng, Liang Zhou, Jianwu Zhao, Zhenzhen Li, Changchun Wang, Shan Front Immunol Immunology Neuroblastoma(NB) is the most common extracranial solid tumor in childhood, and it is now believed that some patients with NB have an underlying genetic susceptibility, which may be one of the reasons for the multiplicity of NB patients within a family line. Even within the same family, the samples show great variation and can present as ganglioneuroblastoma or even benign ganglioneuroma. The genomics of NB is still unclear and more in-depth studies are needed to reveal its key components. We first performed single-cell RNA sequencing(sc-RNAseq) analysis on clinical specimens of two family neuroblastoma(FNB) and four sporadic NB cases. A complete transcriptional profile of FNB was constructed from 18,394 cells from FNB, and we found that SDHD may be genetically associated with FNB and identified a prognostic related CAF subtype in FNB: Fib-4. Single-cell flux estimation analysis (scFEA) results showed that malignant cells were associated with arginine spermine, oxaloacetate and hypoxanthine, and that malignant cells metabolize lactate at lower levels than T cells. Our study provides new resources and ideas for the development of the genomics of family NB, and the mechanisms of cell-to-cell interactions and communication and the metabolic landscape will provide new therapeutic targets. Frontiers Media S.A. 2023-09-18 /pmc/articles/PMC10543897/ /pubmed/37790931 http://dx.doi.org/10.3389/fimmu.2023.1197773 Text en Copyright © 2023 Zhang, Ma, Liu, Du, Peng, Zhou, Zhao, Li and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Zhang, Yunlong
Ma, Yue
Liu, Qingqing
Du, Yifei
Peng, Liang
Zhou, Jianwu
Zhao, Zhenzhen
Li, Changchun
Wang, Shan
Single-cell transcriptome sequencing reveals tumor heterogeneity in family neuroblastoma
title Single-cell transcriptome sequencing reveals tumor heterogeneity in family neuroblastoma
title_full Single-cell transcriptome sequencing reveals tumor heterogeneity in family neuroblastoma
title_fullStr Single-cell transcriptome sequencing reveals tumor heterogeneity in family neuroblastoma
title_full_unstemmed Single-cell transcriptome sequencing reveals tumor heterogeneity in family neuroblastoma
title_short Single-cell transcriptome sequencing reveals tumor heterogeneity in family neuroblastoma
title_sort single-cell transcriptome sequencing reveals tumor heterogeneity in family neuroblastoma
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10543897/
https://www.ncbi.nlm.nih.gov/pubmed/37790931
http://dx.doi.org/10.3389/fimmu.2023.1197773
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