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Purification of functional mouse skeletal muscle mitochondria using percoll density gradient centrifugation

OBJECTIVE: Our goal was to isolate purified mitochondria from mouse skeletal muscle using a Percoll density gradient and to assess bioenergetic function and purity via Seahorse Extracellular Flux (XF) Analyses and mass spectrometry. RESULTS: Mitochondria isolated from murine quadriceps femoris skele...

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Detalles Bibliográficos
Autores principales:	Anunciado-Koza, Rea Victoria P., Guntur, Anyonya R., Vary, Calvin P., Gartner, Carlos A., Nowak, Madeleine, Koza, Robert A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2023
Materias:	Research Note
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10544150/ https://www.ncbi.nlm.nih.gov/pubmed/37777771 http://dx.doi.org/10.1186/s13104-023-06519-4

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