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Role of serum CAP1 protein in the diagnosis of patients with first-time acute myocardial infarction

The dysregulation of adenylate cyclase-associated protein 1 (CAP1) is associated with a variety of inflammatory conditions. Here, we aimed to assess the role of serum CAP1 protein in predicting acute myocardial infarction (AMI), and to explore its effect and mechanism in vascular endothelial cells i...

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Autores principales: Jin, Dongxia, Li, Ximing, Cong, Hongliang, You, Bingchen, Ma, Yue, Hu, Yuecheng, Zhang, Jingxia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10545083/
https://www.ncbi.nlm.nih.gov/pubmed/37773847
http://dx.doi.org/10.1097/MD.0000000000034700
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author Jin, Dongxia
Li, Ximing
Cong, Hongliang
You, Bingchen
Ma, Yue
Hu, Yuecheng
Zhang, Jingxia
author_facet Jin, Dongxia
Li, Ximing
Cong, Hongliang
You, Bingchen
Ma, Yue
Hu, Yuecheng
Zhang, Jingxia
author_sort Jin, Dongxia
collection PubMed
description The dysregulation of adenylate cyclase-associated protein 1 (CAP1) is associated with a variety of inflammatory conditions. Here, we aimed to assess the role of serum CAP1 protein in predicting acute myocardial infarction (AMI), and to explore its effect and mechanism in vascular endothelial cells injury. ELISA was utilized to detected CAP1 protein expression in serum from 70 patients with first-time AMI at 0, 6, 12, 24, 48 hours and 7 days of the onset of chest pain. Receiver operating characteristic (ROC) curve analysis was administered to analyze the diagnostic power of CAP1 for AMI. The CCK-8 and 5-BrdU assays were applied to measure cell proliferation and inflammation in a model of oxidized low-density lipoprotein (ox-LDL) induced human umbilical vein endothelial cells (HUVEC). Luciferase reporter gene assay and Western blotting were used to assess the activity of NF-κB pathway. Results showed that serum CAP1 protein expression was upregulated in patients with first-time AMI, its expression was highest at 12 hours of the onset of chest pain. CAP1 protein was positively associated with the levels of cTnI and ox-LDL. CAP1 showed a relatively high diagnostic accuracy in patients with first-time AMI compared with cTnI, and CAP1 combined with cTnI had superior diagnostic value than CAP1 and cTnI alone. The expression of CAP1 protein was increased in supernatants of ox-LDL induced HUVEC in a dose- and time-dependent manner. CAP1 inhibited cell proliferation but promoted inflammation, and induced the activation of NF-κB pathway in vitro. To sum up, increased serum CAP1 expression might serve as a novel diagnostic biomarker for patients with first-time AMI, the mechanism might be related to its induction of NF-κB pathway activation causing abnormal proliferation and inflammation and thus mediating vascular endothelial cell injury.
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spelling pubmed-105450832023-10-03 Role of serum CAP1 protein in the diagnosis of patients with first-time acute myocardial infarction Jin, Dongxia Li, Ximing Cong, Hongliang You, Bingchen Ma, Yue Hu, Yuecheng Zhang, Jingxia Medicine (Baltimore) 3400 The dysregulation of adenylate cyclase-associated protein 1 (CAP1) is associated with a variety of inflammatory conditions. Here, we aimed to assess the role of serum CAP1 protein in predicting acute myocardial infarction (AMI), and to explore its effect and mechanism in vascular endothelial cells injury. ELISA was utilized to detected CAP1 protein expression in serum from 70 patients with first-time AMI at 0, 6, 12, 24, 48 hours and 7 days of the onset of chest pain. Receiver operating characteristic (ROC) curve analysis was administered to analyze the diagnostic power of CAP1 for AMI. The CCK-8 and 5-BrdU assays were applied to measure cell proliferation and inflammation in a model of oxidized low-density lipoprotein (ox-LDL) induced human umbilical vein endothelial cells (HUVEC). Luciferase reporter gene assay and Western blotting were used to assess the activity of NF-κB pathway. Results showed that serum CAP1 protein expression was upregulated in patients with first-time AMI, its expression was highest at 12 hours of the onset of chest pain. CAP1 protein was positively associated with the levels of cTnI and ox-LDL. CAP1 showed a relatively high diagnostic accuracy in patients with first-time AMI compared with cTnI, and CAP1 combined with cTnI had superior diagnostic value than CAP1 and cTnI alone. The expression of CAP1 protein was increased in supernatants of ox-LDL induced HUVEC in a dose- and time-dependent manner. CAP1 inhibited cell proliferation but promoted inflammation, and induced the activation of NF-κB pathway in vitro. To sum up, increased serum CAP1 expression might serve as a novel diagnostic biomarker for patients with first-time AMI, the mechanism might be related to its induction of NF-κB pathway activation causing abnormal proliferation and inflammation and thus mediating vascular endothelial cell injury. Lippincott Williams & Wilkins 2023-09-29 /pmc/articles/PMC10545083/ /pubmed/37773847 http://dx.doi.org/10.1097/MD.0000000000034700 Text en Copyright © 2023 the Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial License 4.0 (CCBY-NC) (https://creativecommons.org/licenses/by-nc/4.0/) , where it is permissible to download, share, remix, transform, and buildup the work provided it is properly cited. The work cannot be used commercially without permission from the journal.
spellingShingle 3400
Jin, Dongxia
Li, Ximing
Cong, Hongliang
You, Bingchen
Ma, Yue
Hu, Yuecheng
Zhang, Jingxia
Role of serum CAP1 protein in the diagnosis of patients with first-time acute myocardial infarction
title Role of serum CAP1 protein in the diagnosis of patients with first-time acute myocardial infarction
title_full Role of serum CAP1 protein in the diagnosis of patients with first-time acute myocardial infarction
title_fullStr Role of serum CAP1 protein in the diagnosis of patients with first-time acute myocardial infarction
title_full_unstemmed Role of serum CAP1 protein in the diagnosis of patients with first-time acute myocardial infarction
title_short Role of serum CAP1 protein in the diagnosis of patients with first-time acute myocardial infarction
title_sort role of serum cap1 protein in the diagnosis of patients with first-time acute myocardial infarction
topic 3400
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10545083/
https://www.ncbi.nlm.nih.gov/pubmed/37773847
http://dx.doi.org/10.1097/MD.0000000000034700
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