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Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease
The blood-sucking hemipteran Rhodnius prolixus is one of the main vectors of Chagas disease, a neglected tropical disease that affects several million people worldwide. Consuming a blood meal and mating are events with a high epidemiological impact since after each meal, mated females can lay fertil...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10545099/ https://www.ncbi.nlm.nih.gov/pubmed/37729234 http://dx.doi.org/10.1371/journal.pntd.0011640 |
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author | Leyria, Jimena Guarneri, Alessandra A. Lorenzo, Marcelo G. Nouzova, Marcela Noriega, Fernando G. Benrabaa, Samiha A. M. Fernandez-Lima, Francisco Valadares Tose, Lilian Orchard, Ian Lange, Angela B. |
author_facet | Leyria, Jimena Guarneri, Alessandra A. Lorenzo, Marcelo G. Nouzova, Marcela Noriega, Fernando G. Benrabaa, Samiha A. M. Fernandez-Lima, Francisco Valadares Tose, Lilian Orchard, Ian Lange, Angela B. |
author_sort | Leyria, Jimena |
collection | PubMed |
description | The blood-sucking hemipteran Rhodnius prolixus is one of the main vectors of Chagas disease, a neglected tropical disease that affects several million people worldwide. Consuming a blood meal and mating are events with a high epidemiological impact since after each meal, mated females can lay fertile eggs that result in hundreds of offspring. Thus, a better knowledge of the control of R. prolixus reproductive capacity may provide targets for developing novel strategies to control vector populations, thereby reducing vector-host contacts and disease transmission. Here, we have used a combination of gene transcript expression analysis, biochemical assays, hormone measurements and studies of locomotory activity to investigate how mating influences egg development and egg laying rates in R. prolixus females. The results demonstrate that a blood meal increases egg production capacity and leads to earlier egg laying in mated females compared to virgins. Virgin females, however, have increased survival rate over mated females. Circulating juvenile hormone (JH) and ecdysteroid titers are increased in mated females, a process mainly driven through an upregulation of the transcripts for their biosynthetic enzymes in the corpus allatum and ovaries, respectively. Mated females display weaker locomotory activity compared to virgin females, mainly during the photophase. In essence, this study shows how reproductive output and behaviour are profoundly influenced by mating, highlighting molecular, biochemical, endocrine and behavioral features differentially expressed in mated and virgin R. prolixus females. |
format | Online Article Text |
id | pubmed-10545099 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-105450992023-10-03 Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease Leyria, Jimena Guarneri, Alessandra A. Lorenzo, Marcelo G. Nouzova, Marcela Noriega, Fernando G. Benrabaa, Samiha A. M. Fernandez-Lima, Francisco Valadares Tose, Lilian Orchard, Ian Lange, Angela B. PLoS Negl Trop Dis Research Article The blood-sucking hemipteran Rhodnius prolixus is one of the main vectors of Chagas disease, a neglected tropical disease that affects several million people worldwide. Consuming a blood meal and mating are events with a high epidemiological impact since after each meal, mated females can lay fertile eggs that result in hundreds of offspring. Thus, a better knowledge of the control of R. prolixus reproductive capacity may provide targets for developing novel strategies to control vector populations, thereby reducing vector-host contacts and disease transmission. Here, we have used a combination of gene transcript expression analysis, biochemical assays, hormone measurements and studies of locomotory activity to investigate how mating influences egg development and egg laying rates in R. prolixus females. The results demonstrate that a blood meal increases egg production capacity and leads to earlier egg laying in mated females compared to virgins. Virgin females, however, have increased survival rate over mated females. Circulating juvenile hormone (JH) and ecdysteroid titers are increased in mated females, a process mainly driven through an upregulation of the transcripts for their biosynthetic enzymes in the corpus allatum and ovaries, respectively. Mated females display weaker locomotory activity compared to virgin females, mainly during the photophase. In essence, this study shows how reproductive output and behaviour are profoundly influenced by mating, highlighting molecular, biochemical, endocrine and behavioral features differentially expressed in mated and virgin R. prolixus females. Public Library of Science 2023-09-20 /pmc/articles/PMC10545099/ /pubmed/37729234 http://dx.doi.org/10.1371/journal.pntd.0011640 Text en © 2023 Leyria et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Leyria, Jimena Guarneri, Alessandra A. Lorenzo, Marcelo G. Nouzova, Marcela Noriega, Fernando G. Benrabaa, Samiha A. M. Fernandez-Lima, Francisco Valadares Tose, Lilian Orchard, Ian Lange, Angela B. Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease |
title | Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease |
title_full | Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease |
title_fullStr | Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease |
title_full_unstemmed | Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease |
title_short | Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease |
title_sort | effects of mating on female reproductive physiology in the insect model, rhodnius prolixus, a vector of the causative parasite of chagas disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10545099/ https://www.ncbi.nlm.nih.gov/pubmed/37729234 http://dx.doi.org/10.1371/journal.pntd.0011640 |
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