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Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease

The blood-sucking hemipteran Rhodnius prolixus is one of the main vectors of Chagas disease, a neglected tropical disease that affects several million people worldwide. Consuming a blood meal and mating are events with a high epidemiological impact since after each meal, mated females can lay fertil...

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Autores principales: Leyria, Jimena, Guarneri, Alessandra A., Lorenzo, Marcelo G., Nouzova, Marcela, Noriega, Fernando G., Benrabaa, Samiha A. M., Fernandez-Lima, Francisco, Valadares Tose, Lilian, Orchard, Ian, Lange, Angela B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10545099/
https://www.ncbi.nlm.nih.gov/pubmed/37729234
http://dx.doi.org/10.1371/journal.pntd.0011640
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author Leyria, Jimena
Guarneri, Alessandra A.
Lorenzo, Marcelo G.
Nouzova, Marcela
Noriega, Fernando G.
Benrabaa, Samiha A. M.
Fernandez-Lima, Francisco
Valadares Tose, Lilian
Orchard, Ian
Lange, Angela B.
author_facet Leyria, Jimena
Guarneri, Alessandra A.
Lorenzo, Marcelo G.
Nouzova, Marcela
Noriega, Fernando G.
Benrabaa, Samiha A. M.
Fernandez-Lima, Francisco
Valadares Tose, Lilian
Orchard, Ian
Lange, Angela B.
author_sort Leyria, Jimena
collection PubMed
description The blood-sucking hemipteran Rhodnius prolixus is one of the main vectors of Chagas disease, a neglected tropical disease that affects several million people worldwide. Consuming a blood meal and mating are events with a high epidemiological impact since after each meal, mated females can lay fertile eggs that result in hundreds of offspring. Thus, a better knowledge of the control of R. prolixus reproductive capacity may provide targets for developing novel strategies to control vector populations, thereby reducing vector-host contacts and disease transmission. Here, we have used a combination of gene transcript expression analysis, biochemical assays, hormone measurements and studies of locomotory activity to investigate how mating influences egg development and egg laying rates in R. prolixus females. The results demonstrate that a blood meal increases egg production capacity and leads to earlier egg laying in mated females compared to virgins. Virgin females, however, have increased survival rate over mated females. Circulating juvenile hormone (JH) and ecdysteroid titers are increased in mated females, a process mainly driven through an upregulation of the transcripts for their biosynthetic enzymes in the corpus allatum and ovaries, respectively. Mated females display weaker locomotory activity compared to virgin females, mainly during the photophase. In essence, this study shows how reproductive output and behaviour are profoundly influenced by mating, highlighting molecular, biochemical, endocrine and behavioral features differentially expressed in mated and virgin R. prolixus females.
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spelling pubmed-105450992023-10-03 Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease Leyria, Jimena Guarneri, Alessandra A. Lorenzo, Marcelo G. Nouzova, Marcela Noriega, Fernando G. Benrabaa, Samiha A. M. Fernandez-Lima, Francisco Valadares Tose, Lilian Orchard, Ian Lange, Angela B. PLoS Negl Trop Dis Research Article The blood-sucking hemipteran Rhodnius prolixus is one of the main vectors of Chagas disease, a neglected tropical disease that affects several million people worldwide. Consuming a blood meal and mating are events with a high epidemiological impact since after each meal, mated females can lay fertile eggs that result in hundreds of offspring. Thus, a better knowledge of the control of R. prolixus reproductive capacity may provide targets for developing novel strategies to control vector populations, thereby reducing vector-host contacts and disease transmission. Here, we have used a combination of gene transcript expression analysis, biochemical assays, hormone measurements and studies of locomotory activity to investigate how mating influences egg development and egg laying rates in R. prolixus females. The results demonstrate that a blood meal increases egg production capacity and leads to earlier egg laying in mated females compared to virgins. Virgin females, however, have increased survival rate over mated females. Circulating juvenile hormone (JH) and ecdysteroid titers are increased in mated females, a process mainly driven through an upregulation of the transcripts for their biosynthetic enzymes in the corpus allatum and ovaries, respectively. Mated females display weaker locomotory activity compared to virgin females, mainly during the photophase. In essence, this study shows how reproductive output and behaviour are profoundly influenced by mating, highlighting molecular, biochemical, endocrine and behavioral features differentially expressed in mated and virgin R. prolixus females. Public Library of Science 2023-09-20 /pmc/articles/PMC10545099/ /pubmed/37729234 http://dx.doi.org/10.1371/journal.pntd.0011640 Text en © 2023 Leyria et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Leyria, Jimena
Guarneri, Alessandra A.
Lorenzo, Marcelo G.
Nouzova, Marcela
Noriega, Fernando G.
Benrabaa, Samiha A. M.
Fernandez-Lima, Francisco
Valadares Tose, Lilian
Orchard, Ian
Lange, Angela B.
Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease
title Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease
title_full Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease
title_fullStr Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease
title_full_unstemmed Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease
title_short Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease
title_sort effects of mating on female reproductive physiology in the insect model, rhodnius prolixus, a vector of the causative parasite of chagas disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10545099/
https://www.ncbi.nlm.nih.gov/pubmed/37729234
http://dx.doi.org/10.1371/journal.pntd.0011640
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