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Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography
Durable and standardized phantoms with optical properties similar to native healthy and disease-like biological tissues are essential tools for the development, performance testing, calibration and comparison of label-free high-resolution optical coherence tomography (HR-OCT) systems. Available phan...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optica Publishing Group
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10545187/ https://www.ncbi.nlm.nih.gov/pubmed/37791268 http://dx.doi.org/10.1364/BOE.494271 |
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author | Barroso, Álvaro Ketelhut, Steffi Nettels-Hackert, Gerburg Heiduschka, Peter del Amor, Rocío Naranjo, Valery Kemper, Björn Schnekenburger, Jürgen |
author_facet | Barroso, Álvaro Ketelhut, Steffi Nettels-Hackert, Gerburg Heiduschka, Peter del Amor, Rocío Naranjo, Valery Kemper, Björn Schnekenburger, Jürgen |
author_sort | Barroso, Álvaro |
collection | PubMed |
description | Durable and standardized phantoms with optical properties similar to native healthy and disease-like biological tissues are essential tools for the development, performance testing, calibration and comparison of label-free high-resolution optical coherence tomography (HR-OCT) systems. Available phantoms are based on artificial materials and reflect thus only partially ocular properties. To address this limitation, we have performed investigations on the establishment of durable tissue phantoms from ex vivo mouse retina for enhanced reproduction of in vivo structure and complexity. In a proof-of-concept study, we explored the establishment of durable 3D models from dissected mouse eyes that reproduce the properties of normal retina structures and tissue with glaucoma-like layer thickness alterations. We explored different sectioning and preparation procedures for embedding normal and N-methyl-D-aspartate (NMDA)-treated mouse retina in transparent gel matrices and epoxy resins, to generate durable three-dimensional tissue models. Sample quality and reproducibility were quantified by thickness determination of the generated layered structures utilizing computer-assisted segmentation of OCT B-scans that were acquired with a commercial HR-OCT system at a central wavelength of 905 nm and analyzed with custom build software. Our results show that the generated 3D models feature thin biological layers close to current OCT resolution limits and glaucoma-like tissue alterations that are suitable for reliable HR-OCT performance characterization. The comparison of data from resin-embedded tissue with native murine retina in gels demonstrates that by utilization of appropriate preparation protocols, highly stable samples with layered structures equivalent to native tissues can be fabricated. The experimental data demonstrate our concept as a promising approach toward the fabrication of durable biological 3D models suitable for high-resolution OCT system performance characterization supporting the development of optimized instruments for ophthalmology applications. |
format | Online Article Text |
id | pubmed-10545187 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Optica Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-105451872023-10-03 Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography Barroso, Álvaro Ketelhut, Steffi Nettels-Hackert, Gerburg Heiduschka, Peter del Amor, Rocío Naranjo, Valery Kemper, Björn Schnekenburger, Jürgen Biomed Opt Express Article Durable and standardized phantoms with optical properties similar to native healthy and disease-like biological tissues are essential tools for the development, performance testing, calibration and comparison of label-free high-resolution optical coherence tomography (HR-OCT) systems. Available phantoms are based on artificial materials and reflect thus only partially ocular properties. To address this limitation, we have performed investigations on the establishment of durable tissue phantoms from ex vivo mouse retina for enhanced reproduction of in vivo structure and complexity. In a proof-of-concept study, we explored the establishment of durable 3D models from dissected mouse eyes that reproduce the properties of normal retina structures and tissue with glaucoma-like layer thickness alterations. We explored different sectioning and preparation procedures for embedding normal and N-methyl-D-aspartate (NMDA)-treated mouse retina in transparent gel matrices and epoxy resins, to generate durable three-dimensional tissue models. Sample quality and reproducibility were quantified by thickness determination of the generated layered structures utilizing computer-assisted segmentation of OCT B-scans that were acquired with a commercial HR-OCT system at a central wavelength of 905 nm and analyzed with custom build software. Our results show that the generated 3D models feature thin biological layers close to current OCT resolution limits and glaucoma-like tissue alterations that are suitable for reliable HR-OCT performance characterization. The comparison of data from resin-embedded tissue with native murine retina in gels demonstrates that by utilization of appropriate preparation protocols, highly stable samples with layered structures equivalent to native tissues can be fabricated. The experimental data demonstrate our concept as a promising approach toward the fabrication of durable biological 3D models suitable for high-resolution OCT system performance characterization supporting the development of optimized instruments for ophthalmology applications. Optica Publishing Group 2023-08-02 /pmc/articles/PMC10545187/ /pubmed/37791268 http://dx.doi.org/10.1364/BOE.494271 Text en Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Barroso, Álvaro Ketelhut, Steffi Nettels-Hackert, Gerburg Heiduschka, Peter del Amor, Rocío Naranjo, Valery Kemper, Björn Schnekenburger, Jürgen Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography |
title | Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography |
title_full | Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography |
title_fullStr | Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography |
title_full_unstemmed | Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography |
title_short | Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography |
title_sort | durable 3d murine ex vivo retina glaucoma models for optical coherence tomography |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10545187/ https://www.ncbi.nlm.nih.gov/pubmed/37791268 http://dx.doi.org/10.1364/BOE.494271 |
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