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Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography

Durable and standardized phantoms with optical properties similar to native healthy and disease-like biological tissues are essential tools for the development, performance testing, calibration and comparison of label-free high-resolution optical coherence tomography (HR-OCT) systems. Available phan...

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Autores principales: Barroso, Álvaro, Ketelhut, Steffi, Nettels-Hackert, Gerburg, Heiduschka, Peter, del Amor, Rocío, Naranjo, Valery, Kemper, Björn, Schnekenburger, Jürgen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optica Publishing Group 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10545187/
https://www.ncbi.nlm.nih.gov/pubmed/37791268
http://dx.doi.org/10.1364/BOE.494271
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author Barroso, Álvaro
Ketelhut, Steffi
Nettels-Hackert, Gerburg
Heiduschka, Peter
del Amor, Rocío
Naranjo, Valery
Kemper, Björn
Schnekenburger, Jürgen
author_facet Barroso, Álvaro
Ketelhut, Steffi
Nettels-Hackert, Gerburg
Heiduschka, Peter
del Amor, Rocío
Naranjo, Valery
Kemper, Björn
Schnekenburger, Jürgen
author_sort Barroso, Álvaro
collection PubMed
description Durable and standardized phantoms with optical properties similar to native healthy and disease-like biological tissues are essential tools for the development, performance testing, calibration and comparison of label-free high-resolution optical coherence tomography (HR-OCT) systems. Available phantoms are based on artificial materials and reflect thus only partially ocular properties. To address this limitation, we have performed investigations on the establishment of durable tissue phantoms from ex vivo mouse retina for enhanced reproduction of in vivo structure and complexity. In a proof-of-concept study, we explored the establishment of durable 3D models from dissected mouse eyes that reproduce the properties of normal retina structures and tissue with glaucoma-like layer thickness alterations. We explored different sectioning and preparation procedures for embedding normal and N-methyl-D-aspartate (NMDA)-treated mouse retina in transparent gel matrices and epoxy resins, to generate durable three-dimensional tissue models. Sample quality and reproducibility were quantified by thickness determination of the generated layered structures utilizing computer-assisted segmentation of OCT B-scans that were acquired with a commercial HR-OCT system at a central wavelength of 905 nm and analyzed with custom build software. Our results show that the generated 3D models feature thin biological layers close to current OCT resolution limits and glaucoma-like tissue alterations that are suitable for reliable HR-OCT performance characterization. The comparison of data from resin-embedded tissue with native murine retina in gels demonstrates that by utilization of appropriate preparation protocols, highly stable samples with layered structures equivalent to native tissues can be fabricated. The experimental data demonstrate our concept as a promising approach toward the fabrication of durable biological 3D models suitable for high-resolution OCT system performance characterization supporting the development of optimized instruments for ophthalmology applications.
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spelling pubmed-105451872023-10-03 Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography Barroso, Álvaro Ketelhut, Steffi Nettels-Hackert, Gerburg Heiduschka, Peter del Amor, Rocío Naranjo, Valery Kemper, Björn Schnekenburger, Jürgen Biomed Opt Express Article Durable and standardized phantoms with optical properties similar to native healthy and disease-like biological tissues are essential tools for the development, performance testing, calibration and comparison of label-free high-resolution optical coherence tomography (HR-OCT) systems. Available phantoms are based on artificial materials and reflect thus only partially ocular properties. To address this limitation, we have performed investigations on the establishment of durable tissue phantoms from ex vivo mouse retina for enhanced reproduction of in vivo structure and complexity. In a proof-of-concept study, we explored the establishment of durable 3D models from dissected mouse eyes that reproduce the properties of normal retina structures and tissue with glaucoma-like layer thickness alterations. We explored different sectioning and preparation procedures for embedding normal and N-methyl-D-aspartate (NMDA)-treated mouse retina in transparent gel matrices and epoxy resins, to generate durable three-dimensional tissue models. Sample quality and reproducibility were quantified by thickness determination of the generated layered structures utilizing computer-assisted segmentation of OCT B-scans that were acquired with a commercial HR-OCT system at a central wavelength of 905 nm and analyzed with custom build software. Our results show that the generated 3D models feature thin biological layers close to current OCT resolution limits and glaucoma-like tissue alterations that are suitable for reliable HR-OCT performance characterization. The comparison of data from resin-embedded tissue with native murine retina in gels demonstrates that by utilization of appropriate preparation protocols, highly stable samples with layered structures equivalent to native tissues can be fabricated. The experimental data demonstrate our concept as a promising approach toward the fabrication of durable biological 3D models suitable for high-resolution OCT system performance characterization supporting the development of optimized instruments for ophthalmology applications. Optica Publishing Group 2023-08-02 /pmc/articles/PMC10545187/ /pubmed/37791268 http://dx.doi.org/10.1364/BOE.494271 Text en Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Barroso, Álvaro
Ketelhut, Steffi
Nettels-Hackert, Gerburg
Heiduschka, Peter
del Amor, Rocío
Naranjo, Valery
Kemper, Björn
Schnekenburger, Jürgen
Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography
title Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography
title_full Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography
title_fullStr Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography
title_full_unstemmed Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography
title_short Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography
title_sort durable 3d murine ex vivo retina glaucoma models for optical coherence tomography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10545187/
https://www.ncbi.nlm.nih.gov/pubmed/37791268
http://dx.doi.org/10.1364/BOE.494271
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