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Validation of the volumetric flow cytometry for bovine sperm concentration
Sperm concentration is a stronghold of the andrological evaluation and the production of insemination doses. The use of haemocytometers, although considered the gold standard, is difficult to apply in field conditions because it is subjective and time-consuming. The present study was designed to val...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10545691/ https://www.ncbi.nlm.nih.gov/pubmed/37783767 http://dx.doi.org/10.1038/s41598-023-43678-7 |
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author | Gloria, Alessia Bracco, Claudia Antenucci, Emiliana Contri, Alberto |
author_facet | Gloria, Alessia Bracco, Claudia Antenucci, Emiliana Contri, Alberto |
author_sort | Gloria, Alessia |
collection | PubMed |
description | Sperm concentration is a stronghold of the andrological evaluation and the production of insemination doses. The use of haemocytometers, although considered the gold standard, is difficult to apply in field conditions because it is subjective and time-consuming. The present study was designed to validate the volumetric flow cytometry (volFC) in order to estimate bovine sperm concentration, comparing it with the performances of haemocytometer, NucleoCounter, and flow cytometry with the use of fluorospheres. Compared with other methods, volFC appeared less affected by large dilution of the sample, with similar concentrations calculated in the range of dilution 1:200–1:800. Using volFc the population detected on the basis of morphological criteria and fluorescence of DNA better represents the real concentration of sperm in the sample. The volFC showed high repeatability compared with the haemocytometer (coefficient of variation 1.85% and 4.52%, respectively) and stable performances with cryopreserved samples, with negligible effects of the medium components. The present study showed that volFC is as accurate and precise as other techniques to estimate sperm concentration in bovine fresh and frozen semen, but it is less affected by operative conditions, such as sample dilution. The possibility to quantify sperm functional subpopulations by volFC could potentially implement the study of the relationship between sperm attributes and fertility. |
format | Online Article Text |
id | pubmed-10545691 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-105456912023-10-04 Validation of the volumetric flow cytometry for bovine sperm concentration Gloria, Alessia Bracco, Claudia Antenucci, Emiliana Contri, Alberto Sci Rep Article Sperm concentration is a stronghold of the andrological evaluation and the production of insemination doses. The use of haemocytometers, although considered the gold standard, is difficult to apply in field conditions because it is subjective and time-consuming. The present study was designed to validate the volumetric flow cytometry (volFC) in order to estimate bovine sperm concentration, comparing it with the performances of haemocytometer, NucleoCounter, and flow cytometry with the use of fluorospheres. Compared with other methods, volFC appeared less affected by large dilution of the sample, with similar concentrations calculated in the range of dilution 1:200–1:800. Using volFc the population detected on the basis of morphological criteria and fluorescence of DNA better represents the real concentration of sperm in the sample. The volFC showed high repeatability compared with the haemocytometer (coefficient of variation 1.85% and 4.52%, respectively) and stable performances with cryopreserved samples, with negligible effects of the medium components. The present study showed that volFC is as accurate and precise as other techniques to estimate sperm concentration in bovine fresh and frozen semen, but it is less affected by operative conditions, such as sample dilution. The possibility to quantify sperm functional subpopulations by volFC could potentially implement the study of the relationship between sperm attributes and fertility. Nature Publishing Group UK 2023-10-02 /pmc/articles/PMC10545691/ /pubmed/37783767 http://dx.doi.org/10.1038/s41598-023-43678-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Gloria, Alessia Bracco, Claudia Antenucci, Emiliana Contri, Alberto Validation of the volumetric flow cytometry for bovine sperm concentration |
title | Validation of the volumetric flow cytometry for bovine sperm concentration |
title_full | Validation of the volumetric flow cytometry for bovine sperm concentration |
title_fullStr | Validation of the volumetric flow cytometry for bovine sperm concentration |
title_full_unstemmed | Validation of the volumetric flow cytometry for bovine sperm concentration |
title_short | Validation of the volumetric flow cytometry for bovine sperm concentration |
title_sort | validation of the volumetric flow cytometry for bovine sperm concentration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10545691/ https://www.ncbi.nlm.nih.gov/pubmed/37783767 http://dx.doi.org/10.1038/s41598-023-43678-7 |
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