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Polystyrene-colonizing bacteria are enriched for long-chain alkane degradation pathways
One of the most promising strategies for the management of plastic waste is microbial biodegradation, but efficient degraders for many types of plastics are still lacking, including those for polystyrene (PS). Genomics has emerged as a powerful tool for mining environmental microbes that may have th...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10547174/ https://www.ncbi.nlm.nih.gov/pubmed/37788234 http://dx.doi.org/10.1371/journal.pone.0292137 |
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author | Hsueh, Shu Wei Jian, You-Hua Fugmann, Sebastian D. Yang, Shu Yuan |
author_facet | Hsueh, Shu Wei Jian, You-Hua Fugmann, Sebastian D. Yang, Shu Yuan |
author_sort | Hsueh, Shu Wei |
collection | PubMed |
description | One of the most promising strategies for the management of plastic waste is microbial biodegradation, but efficient degraders for many types of plastics are still lacking, including those for polystyrene (PS). Genomics has emerged as a powerful tool for mining environmental microbes that may have the ability to degrade different types of plastics. In this study, we use 16S rRNA sequencing to analyze the microbiomes for multiple PS samples collected from sites with different vegetation in Taiwan to reveal potential common properties between species that exhibit growth advantages on PS surfaces. Phylum enrichment analysis identified Cyanobacteria and Deinococcus-Thermus as being the most over-represented groups on PS, and both phyla include species known to reside in extreme environments and could encode unique enzymes that grant them properties suitable for colonization on PS surfaces. Investigation of functional enrichment using reference genomes of PS-enriched species highlighted carbon metabolic pathways, especially those related to hydrocarbon degradation. This is corroborated by the finding that genes encoding long-chain alkane hydroxylases such as AlmA are more prevalent in the genomes of PS-associated bacteria. Our analyses illustrate how plastic in the environment support the colonization by different microbes compared to surrounding soil. In addition, our results point to the possibility that alkane hydroxylases could confer growth advantages of microbes on PS. |
format | Online Article Text |
id | pubmed-10547174 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-105471742023-10-04 Polystyrene-colonizing bacteria are enriched for long-chain alkane degradation pathways Hsueh, Shu Wei Jian, You-Hua Fugmann, Sebastian D. Yang, Shu Yuan PLoS One Research Article One of the most promising strategies for the management of plastic waste is microbial biodegradation, but efficient degraders for many types of plastics are still lacking, including those for polystyrene (PS). Genomics has emerged as a powerful tool for mining environmental microbes that may have the ability to degrade different types of plastics. In this study, we use 16S rRNA sequencing to analyze the microbiomes for multiple PS samples collected from sites with different vegetation in Taiwan to reveal potential common properties between species that exhibit growth advantages on PS surfaces. Phylum enrichment analysis identified Cyanobacteria and Deinococcus-Thermus as being the most over-represented groups on PS, and both phyla include species known to reside in extreme environments and could encode unique enzymes that grant them properties suitable for colonization on PS surfaces. Investigation of functional enrichment using reference genomes of PS-enriched species highlighted carbon metabolic pathways, especially those related to hydrocarbon degradation. This is corroborated by the finding that genes encoding long-chain alkane hydroxylases such as AlmA are more prevalent in the genomes of PS-associated bacteria. Our analyses illustrate how plastic in the environment support the colonization by different microbes compared to surrounding soil. In addition, our results point to the possibility that alkane hydroxylases could confer growth advantages of microbes on PS. Public Library of Science 2023-10-03 /pmc/articles/PMC10547174/ /pubmed/37788234 http://dx.doi.org/10.1371/journal.pone.0292137 Text en © 2023 Hsueh et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Hsueh, Shu Wei Jian, You-Hua Fugmann, Sebastian D. Yang, Shu Yuan Polystyrene-colonizing bacteria are enriched for long-chain alkane degradation pathways |
title | Polystyrene-colonizing bacteria are enriched for long-chain alkane degradation pathways |
title_full | Polystyrene-colonizing bacteria are enriched for long-chain alkane degradation pathways |
title_fullStr | Polystyrene-colonizing bacteria are enriched for long-chain alkane degradation pathways |
title_full_unstemmed | Polystyrene-colonizing bacteria are enriched for long-chain alkane degradation pathways |
title_short | Polystyrene-colonizing bacteria are enriched for long-chain alkane degradation pathways |
title_sort | polystyrene-colonizing bacteria are enriched for long-chain alkane degradation pathways |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10547174/ https://www.ncbi.nlm.nih.gov/pubmed/37788234 http://dx.doi.org/10.1371/journal.pone.0292137 |
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