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Cooperative motility, force generation and mechanosensing in a foraging non-photosynthetic diatom

Diatoms are ancestrally photosynthetic microalgae. However, some underwent a major evolutionary transition, losing photosynthesis to become obligate heterotrophs. The molecular and physiological basis for this transition is unclear. Here, we isolate and characterize new strains of non-photosynthetic...

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Autores principales: Zheng, Peng, Kumadaki, Kayo, Quek, Christopher, Lim, Zeng Hao, Ashenafi, Yonatan, Yip, Zhi Ting, Newby, Jay, Alverson, Andrew J., Jie, Yan, Jedd, Gregory
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10547550/
https://www.ncbi.nlm.nih.gov/pubmed/37788707
http://dx.doi.org/10.1098/rsob.230148
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author Zheng, Peng
Kumadaki, Kayo
Quek, Christopher
Lim, Zeng Hao
Ashenafi, Yonatan
Yip, Zhi Ting
Newby, Jay
Alverson, Andrew J.
Jie, Yan
Jedd, Gregory
author_facet Zheng, Peng
Kumadaki, Kayo
Quek, Christopher
Lim, Zeng Hao
Ashenafi, Yonatan
Yip, Zhi Ting
Newby, Jay
Alverson, Andrew J.
Jie, Yan
Jedd, Gregory
author_sort Zheng, Peng
collection PubMed
description Diatoms are ancestrally photosynthetic microalgae. However, some underwent a major evolutionary transition, losing photosynthesis to become obligate heterotrophs. The molecular and physiological basis for this transition is unclear. Here, we isolate and characterize new strains of non-photosynthetic diatoms from the coastal waters of Singapore. These diatoms occupy diverse ecological niches and display glucose-mediated catabolite repression, a classical feature of bacterial and fungal heterotrophs. Live-cell imaging reveals deposition of secreted extracellular polymeric substance (EPS). Diatoms moving on pre-existing EPS trails (runners) move faster than those laying new trails (blazers). This leads to cell-to-cell coupling where runners can push blazers to make them move faster. Calibrated micropipettes measure substantial single-cell pushing forces, which are consistent with high-order myosin motor cooperativity. Collisions that impede forward motion induce reversal, revealing navigation-related force sensing. Together, these data identify aspects of metabolism and motility that are likely to promote and underpin diatom heterotrophy.
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spelling pubmed-105475502023-10-04 Cooperative motility, force generation and mechanosensing in a foraging non-photosynthetic diatom Zheng, Peng Kumadaki, Kayo Quek, Christopher Lim, Zeng Hao Ashenafi, Yonatan Yip, Zhi Ting Newby, Jay Alverson, Andrew J. Jie, Yan Jedd, Gregory Open Biol Research Diatoms are ancestrally photosynthetic microalgae. However, some underwent a major evolutionary transition, losing photosynthesis to become obligate heterotrophs. The molecular and physiological basis for this transition is unclear. Here, we isolate and characterize new strains of non-photosynthetic diatoms from the coastal waters of Singapore. These diatoms occupy diverse ecological niches and display glucose-mediated catabolite repression, a classical feature of bacterial and fungal heterotrophs. Live-cell imaging reveals deposition of secreted extracellular polymeric substance (EPS). Diatoms moving on pre-existing EPS trails (runners) move faster than those laying new trails (blazers). This leads to cell-to-cell coupling where runners can push blazers to make them move faster. Calibrated micropipettes measure substantial single-cell pushing forces, which are consistent with high-order myosin motor cooperativity. Collisions that impede forward motion induce reversal, revealing navigation-related force sensing. Together, these data identify aspects of metabolism and motility that are likely to promote and underpin diatom heterotrophy. The Royal Society 2023-10-04 /pmc/articles/PMC10547550/ /pubmed/37788707 http://dx.doi.org/10.1098/rsob.230148 Text en © 2023 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited.
spellingShingle Research
Zheng, Peng
Kumadaki, Kayo
Quek, Christopher
Lim, Zeng Hao
Ashenafi, Yonatan
Yip, Zhi Ting
Newby, Jay
Alverson, Andrew J.
Jie, Yan
Jedd, Gregory
Cooperative motility, force generation and mechanosensing in a foraging non-photosynthetic diatom
title Cooperative motility, force generation and mechanosensing in a foraging non-photosynthetic diatom
title_full Cooperative motility, force generation and mechanosensing in a foraging non-photosynthetic diatom
title_fullStr Cooperative motility, force generation and mechanosensing in a foraging non-photosynthetic diatom
title_full_unstemmed Cooperative motility, force generation and mechanosensing in a foraging non-photosynthetic diatom
title_short Cooperative motility, force generation and mechanosensing in a foraging non-photosynthetic diatom
title_sort cooperative motility, force generation and mechanosensing in a foraging non-photosynthetic diatom
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10547550/
https://www.ncbi.nlm.nih.gov/pubmed/37788707
http://dx.doi.org/10.1098/rsob.230148
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