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Cooperative motility, force generation and mechanosensing in a foraging non-photosynthetic diatom
Diatoms are ancestrally photosynthetic microalgae. However, some underwent a major evolutionary transition, losing photosynthesis to become obligate heterotrophs. The molecular and physiological basis for this transition is unclear. Here, we isolate and characterize new strains of non-photosynthetic...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10547550/ https://www.ncbi.nlm.nih.gov/pubmed/37788707 http://dx.doi.org/10.1098/rsob.230148 |
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author | Zheng, Peng Kumadaki, Kayo Quek, Christopher Lim, Zeng Hao Ashenafi, Yonatan Yip, Zhi Ting Newby, Jay Alverson, Andrew J. Jie, Yan Jedd, Gregory |
author_facet | Zheng, Peng Kumadaki, Kayo Quek, Christopher Lim, Zeng Hao Ashenafi, Yonatan Yip, Zhi Ting Newby, Jay Alverson, Andrew J. Jie, Yan Jedd, Gregory |
author_sort | Zheng, Peng |
collection | PubMed |
description | Diatoms are ancestrally photosynthetic microalgae. However, some underwent a major evolutionary transition, losing photosynthesis to become obligate heterotrophs. The molecular and physiological basis for this transition is unclear. Here, we isolate and characterize new strains of non-photosynthetic diatoms from the coastal waters of Singapore. These diatoms occupy diverse ecological niches and display glucose-mediated catabolite repression, a classical feature of bacterial and fungal heterotrophs. Live-cell imaging reveals deposition of secreted extracellular polymeric substance (EPS). Diatoms moving on pre-existing EPS trails (runners) move faster than those laying new trails (blazers). This leads to cell-to-cell coupling where runners can push blazers to make them move faster. Calibrated micropipettes measure substantial single-cell pushing forces, which are consistent with high-order myosin motor cooperativity. Collisions that impede forward motion induce reversal, revealing navigation-related force sensing. Together, these data identify aspects of metabolism and motility that are likely to promote and underpin diatom heterotrophy. |
format | Online Article Text |
id | pubmed-10547550 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-105475502023-10-04 Cooperative motility, force generation and mechanosensing in a foraging non-photosynthetic diatom Zheng, Peng Kumadaki, Kayo Quek, Christopher Lim, Zeng Hao Ashenafi, Yonatan Yip, Zhi Ting Newby, Jay Alverson, Andrew J. Jie, Yan Jedd, Gregory Open Biol Research Diatoms are ancestrally photosynthetic microalgae. However, some underwent a major evolutionary transition, losing photosynthesis to become obligate heterotrophs. The molecular and physiological basis for this transition is unclear. Here, we isolate and characterize new strains of non-photosynthetic diatoms from the coastal waters of Singapore. These diatoms occupy diverse ecological niches and display glucose-mediated catabolite repression, a classical feature of bacterial and fungal heterotrophs. Live-cell imaging reveals deposition of secreted extracellular polymeric substance (EPS). Diatoms moving on pre-existing EPS trails (runners) move faster than those laying new trails (blazers). This leads to cell-to-cell coupling where runners can push blazers to make them move faster. Calibrated micropipettes measure substantial single-cell pushing forces, which are consistent with high-order myosin motor cooperativity. Collisions that impede forward motion induce reversal, revealing navigation-related force sensing. Together, these data identify aspects of metabolism and motility that are likely to promote and underpin diatom heterotrophy. The Royal Society 2023-10-04 /pmc/articles/PMC10547550/ /pubmed/37788707 http://dx.doi.org/10.1098/rsob.230148 Text en © 2023 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Research Zheng, Peng Kumadaki, Kayo Quek, Christopher Lim, Zeng Hao Ashenafi, Yonatan Yip, Zhi Ting Newby, Jay Alverson, Andrew J. Jie, Yan Jedd, Gregory Cooperative motility, force generation and mechanosensing in a foraging non-photosynthetic diatom |
title | Cooperative motility, force generation and mechanosensing in a foraging non-photosynthetic diatom |
title_full | Cooperative motility, force generation and mechanosensing in a foraging non-photosynthetic diatom |
title_fullStr | Cooperative motility, force generation and mechanosensing in a foraging non-photosynthetic diatom |
title_full_unstemmed | Cooperative motility, force generation and mechanosensing in a foraging non-photosynthetic diatom |
title_short | Cooperative motility, force generation and mechanosensing in a foraging non-photosynthetic diatom |
title_sort | cooperative motility, force generation and mechanosensing in a foraging non-photosynthetic diatom |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10547550/ https://www.ncbi.nlm.nih.gov/pubmed/37788707 http://dx.doi.org/10.1098/rsob.230148 |
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