Cargando…
Effect of phospholipid head group on ultrasound-triggered drug release and cellular uptake of immunoliposomes
Liposomes are the most successful nanoparticles used to date to load and deliver chemotherapeutic agents to cancer cells. They are nano-sized vesicles made up of phospholipids, and targeting moieties can be added to their surfaces for the active targeting of specific tumors. Furthermore, Ultrasound...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10547810/ https://www.ncbi.nlm.nih.gov/pubmed/37789072 http://dx.doi.org/10.1038/s41598-023-43813-4 |
_version_ | 1785115136584843264 |
---|---|
author | Awad, Nahid S. Paul, Vinod AlSawaftah, Nour M. Husseini, Ghaleb A. |
author_facet | Awad, Nahid S. Paul, Vinod AlSawaftah, Nour M. Husseini, Ghaleb A. |
author_sort | Awad, Nahid S. |
collection | PubMed |
description | Liposomes are the most successful nanoparticles used to date to load and deliver chemotherapeutic agents to cancer cells. They are nano-sized vesicles made up of phospholipids, and targeting moieties can be added to their surfaces for the active targeting of specific tumors. Furthermore, Ultrasound can be used to trigger the release of the loaded drugs by disturbing their phospholipid bilayer structure. In this study, we have prepared pegylated liposomes using four types of phospholipids with similar saturated hydrocarbon tails including a phospholipid with no head group attached to the phosphate head (DPPA) and three other phospholipids with different head groups attached to their phosphate heads (DPPC, DPPE and DPPG). The prepared liposomes were conjugated to the monoclonal antibody trastuzumab (TRA) to target the human epidermal growth factor receptor 2 (HER2) overexpressed on HER2-positive cancer cells (HER2+). We have compared the response of the different formulations of liposomes when triggered with low-frequency ultrasound (LFUS) and their cellular uptake by the cancer cells. The results showed that the different formulations had similar size, polydispersity, and stability. TRA-conjugated DPPC liposomes showed the highest sensitivity to LFUS. On the other hand, incubating the cancer cells with TRA-conjugated DPPA liposomes triggered with LFUS showed the highest uptake of the loaded calcein by the HER2+ cells. |
format | Online Article Text |
id | pubmed-10547810 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-105478102023-10-05 Effect of phospholipid head group on ultrasound-triggered drug release and cellular uptake of immunoliposomes Awad, Nahid S. Paul, Vinod AlSawaftah, Nour M. Husseini, Ghaleb A. Sci Rep Article Liposomes are the most successful nanoparticles used to date to load and deliver chemotherapeutic agents to cancer cells. They are nano-sized vesicles made up of phospholipids, and targeting moieties can be added to their surfaces for the active targeting of specific tumors. Furthermore, Ultrasound can be used to trigger the release of the loaded drugs by disturbing their phospholipid bilayer structure. In this study, we have prepared pegylated liposomes using four types of phospholipids with similar saturated hydrocarbon tails including a phospholipid with no head group attached to the phosphate head (DPPA) and three other phospholipids with different head groups attached to their phosphate heads (DPPC, DPPE and DPPG). The prepared liposomes were conjugated to the monoclonal antibody trastuzumab (TRA) to target the human epidermal growth factor receptor 2 (HER2) overexpressed on HER2-positive cancer cells (HER2+). We have compared the response of the different formulations of liposomes when triggered with low-frequency ultrasound (LFUS) and their cellular uptake by the cancer cells. The results showed that the different formulations had similar size, polydispersity, and stability. TRA-conjugated DPPC liposomes showed the highest sensitivity to LFUS. On the other hand, incubating the cancer cells with TRA-conjugated DPPA liposomes triggered with LFUS showed the highest uptake of the loaded calcein by the HER2+ cells. Nature Publishing Group UK 2023-10-03 /pmc/articles/PMC10547810/ /pubmed/37789072 http://dx.doi.org/10.1038/s41598-023-43813-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Awad, Nahid S. Paul, Vinod AlSawaftah, Nour M. Husseini, Ghaleb A. Effect of phospholipid head group on ultrasound-triggered drug release and cellular uptake of immunoliposomes |
title | Effect of phospholipid head group on ultrasound-triggered drug release and cellular uptake of immunoliposomes |
title_full | Effect of phospholipid head group on ultrasound-triggered drug release and cellular uptake of immunoliposomes |
title_fullStr | Effect of phospholipid head group on ultrasound-triggered drug release and cellular uptake of immunoliposomes |
title_full_unstemmed | Effect of phospholipid head group on ultrasound-triggered drug release and cellular uptake of immunoliposomes |
title_short | Effect of phospholipid head group on ultrasound-triggered drug release and cellular uptake of immunoliposomes |
title_sort | effect of phospholipid head group on ultrasound-triggered drug release and cellular uptake of immunoliposomes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10547810/ https://www.ncbi.nlm.nih.gov/pubmed/37789072 http://dx.doi.org/10.1038/s41598-023-43813-4 |
work_keys_str_mv | AT awadnahids effectofphospholipidheadgrouponultrasoundtriggereddrugreleaseandcellularuptakeofimmunoliposomes AT paulvinod effectofphospholipidheadgrouponultrasoundtriggereddrugreleaseandcellularuptakeofimmunoliposomes AT alsawaftahnourm effectofphospholipidheadgrouponultrasoundtriggereddrugreleaseandcellularuptakeofimmunoliposomes AT husseinighaleba effectofphospholipidheadgrouponultrasoundtriggereddrugreleaseandcellularuptakeofimmunoliposomes |