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Native circulating Brucella melitensis lineages causing a brucellosis epidemic in Qinghai, China

Since 2010, the cases and incidences of human brucellosis have been increasing annually in Qinghai (QH) Province. Molecular epidemiology and phylogenetic analyses of strains from this region are crucial to better understand the transmission of the disease and the evolutionary patterns of Brucella st...

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Autores principales: Xue, Hongmei, Zhao, Zhijun, Wang, Jianling, Ma, Li, Li, Jiquan, Yang, Xuxin, Ren, Lingling, Xu, Liqing, Liu, Zhiguo, Li, Zhenjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10547896/
https://www.ncbi.nlm.nih.gov/pubmed/37799605
http://dx.doi.org/10.3389/fmicb.2023.1233686
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author Xue, Hongmei
Zhao, Zhijun
Wang, Jianling
Ma, Li
Li, Jiquan
Yang, Xuxin
Ren, Lingling
Xu, Liqing
Liu, Zhiguo
Li, Zhenjun
author_facet Xue, Hongmei
Zhao, Zhijun
Wang, Jianling
Ma, Li
Li, Jiquan
Yang, Xuxin
Ren, Lingling
Xu, Liqing
Liu, Zhiguo
Li, Zhenjun
author_sort Xue, Hongmei
collection PubMed
description Since 2010, the cases and incidences of human brucellosis have been increasing annually in Qinghai (QH) Province. Molecular epidemiology and phylogenetic analyses of strains from this region are crucial to better understand the transmission of the disease and the evolutionary patterns of Brucella strains. In this study, classical bio-typing assay, multilocus variable-number tandem repeat analysis, and the whole-genome sequencing–single-nucleotide polymorphism approach were used to illustrate the epidemiological and evolutionary patterns of Brucella melitensis. A total of 54 B. melitensis bv. 3 strains were isolated and molecularly characterized, with all strains belonging to the East Mediterranean lineages. Cross-regional transmission events (i.e., between counties) were caused by common sources of infection, suggesting that predominant circulating genotypes are endemic in different regions. Strengthening surveillance in animal brucellosis and controlling infected animals’ cross-border movement are necessary. Two strains isolated from humans and marmots were clustered in the same sub-clade, implying the possible existence of direct and/or indirect contact between sheep (and goats) and wildlife (marmots), but this needs to be verified by further investigations. The global-scale phylogenetic analysis indicated that 54 strains sorted into six subclades, four of which formed independent lineages, suggesting that the increase in the incidence rate of human brucellosis may be caused by local circulating lineages. Further strengthening the serology and pathogen surveillance of animals (wildlife) and humans will contribute to an in-depth understanding of the transmission chain of human brucellosis in this region.
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spelling pubmed-105478962023-10-05 Native circulating Brucella melitensis lineages causing a brucellosis epidemic in Qinghai, China Xue, Hongmei Zhao, Zhijun Wang, Jianling Ma, Li Li, Jiquan Yang, Xuxin Ren, Lingling Xu, Liqing Liu, Zhiguo Li, Zhenjun Front Microbiol Microbiology Since 2010, the cases and incidences of human brucellosis have been increasing annually in Qinghai (QH) Province. Molecular epidemiology and phylogenetic analyses of strains from this region are crucial to better understand the transmission of the disease and the evolutionary patterns of Brucella strains. In this study, classical bio-typing assay, multilocus variable-number tandem repeat analysis, and the whole-genome sequencing–single-nucleotide polymorphism approach were used to illustrate the epidemiological and evolutionary patterns of Brucella melitensis. A total of 54 B. melitensis bv. 3 strains were isolated and molecularly characterized, with all strains belonging to the East Mediterranean lineages. Cross-regional transmission events (i.e., between counties) were caused by common sources of infection, suggesting that predominant circulating genotypes are endemic in different regions. Strengthening surveillance in animal brucellosis and controlling infected animals’ cross-border movement are necessary. Two strains isolated from humans and marmots were clustered in the same sub-clade, implying the possible existence of direct and/or indirect contact between sheep (and goats) and wildlife (marmots), but this needs to be verified by further investigations. The global-scale phylogenetic analysis indicated that 54 strains sorted into six subclades, four of which formed independent lineages, suggesting that the increase in the incidence rate of human brucellosis may be caused by local circulating lineages. Further strengthening the serology and pathogen surveillance of animals (wildlife) and humans will contribute to an in-depth understanding of the transmission chain of human brucellosis in this region. Frontiers Media S.A. 2023-09-20 /pmc/articles/PMC10547896/ /pubmed/37799605 http://dx.doi.org/10.3389/fmicb.2023.1233686 Text en Copyright © 2023 Xue, Zhao, Wang, Ma, Li, Yang, Ren, Xu, Liu and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xue, Hongmei
Zhao, Zhijun
Wang, Jianling
Ma, Li
Li, Jiquan
Yang, Xuxin
Ren, Lingling
Xu, Liqing
Liu, Zhiguo
Li, Zhenjun
Native circulating Brucella melitensis lineages causing a brucellosis epidemic in Qinghai, China
title Native circulating Brucella melitensis lineages causing a brucellosis epidemic in Qinghai, China
title_full Native circulating Brucella melitensis lineages causing a brucellosis epidemic in Qinghai, China
title_fullStr Native circulating Brucella melitensis lineages causing a brucellosis epidemic in Qinghai, China
title_full_unstemmed Native circulating Brucella melitensis lineages causing a brucellosis epidemic in Qinghai, China
title_short Native circulating Brucella melitensis lineages causing a brucellosis epidemic in Qinghai, China
title_sort native circulating brucella melitensis lineages causing a brucellosis epidemic in qinghai, china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10547896/
https://www.ncbi.nlm.nih.gov/pubmed/37799605
http://dx.doi.org/10.3389/fmicb.2023.1233686
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