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The maintenance mechanism of hematopoietic stem cell dormancy: role for a subset of macrophages

Hematopoiesis is regulated by crosstalk between long-term repopulating hematopoietic stem cells (LT-HSCs) and supporting niche cells in the bone marrow (BM). Here, we describe the role of KAI1, which is mainly expressed on LT-HSCs and rarely on other hematopoietic stem-progenitor cells (HSPCs), in n...

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Autores principales: Chae, Cheong-Whan, Choi, Gun, Kim, You Ji, Cho, Mingug, Kwon, Yoo-Wook, Kim, Hyo-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10547972/
https://www.ncbi.nlm.nih.gov/pubmed/37574807
http://dx.doi.org/10.5483/BMBRep.2023-0092
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author Chae, Cheong-Whan
Choi, Gun
Kim, You Ji
Cho, Mingug
Kwon, Yoo-Wook
Kim, Hyo-Soo
author_facet Chae, Cheong-Whan
Choi, Gun
Kim, You Ji
Cho, Mingug
Kwon, Yoo-Wook
Kim, Hyo-Soo
author_sort Chae, Cheong-Whan
collection PubMed
description Hematopoiesis is regulated by crosstalk between long-term repopulating hematopoietic stem cells (LT-HSCs) and supporting niche cells in the bone marrow (BM). Here, we describe the role of KAI1, which is mainly expressed on LT-HSCs and rarely on other hematopoietic stem-progenitor cells (HSPCs), in niche-mediated LT-HSC maintenance. KAI1 activates TGF-β1/Smad3 signal in LT-HSCs, leading to the induction of CDK inhibitors and inhibition of the cell cycle. The KAI1-binding partner DARC is expressed on macrophages and stabilizes KAI1 on LT-HSCs, promoting their quiescence. Conversely, when DARC+ BM macrophages were absent, the level of surface KAI1 on LT-HSCs decreases, leading to cell-cycle entry, proliferation, and differentiation. Thus, KAI1 acts as a functional surface marker of LT-HSCs that regulates dormancy through interaction with DARC-expressing macrophages in the BM stem cell niche. Recently, we showed very special and rare macrophages expressing α-SMA+ COX2+ & DARC+ induce not only dormancy of LT-HSC through interaction of KAI1-DARC but also protect HSCs by down-regulating ROS through COX2 signaling. In the near future, the strategy to combine KAI1-positive LT-HSCs and α-SMA/Cox2/DARC triple-positive macrophages will improve the efficacy of stem cell transplantation after the ablative chemo-therapy for hematological disorders including leukemia.
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spelling pubmed-105479722023-10-05 The maintenance mechanism of hematopoietic stem cell dormancy: role for a subset of macrophages Chae, Cheong-Whan Choi, Gun Kim, You Ji Cho, Mingug Kwon, Yoo-Wook Kim, Hyo-Soo BMB Rep Invited Mini Review Hematopoiesis is regulated by crosstalk between long-term repopulating hematopoietic stem cells (LT-HSCs) and supporting niche cells in the bone marrow (BM). Here, we describe the role of KAI1, which is mainly expressed on LT-HSCs and rarely on other hematopoietic stem-progenitor cells (HSPCs), in niche-mediated LT-HSC maintenance. KAI1 activates TGF-β1/Smad3 signal in LT-HSCs, leading to the induction of CDK inhibitors and inhibition of the cell cycle. The KAI1-binding partner DARC is expressed on macrophages and stabilizes KAI1 on LT-HSCs, promoting their quiescence. Conversely, when DARC+ BM macrophages were absent, the level of surface KAI1 on LT-HSCs decreases, leading to cell-cycle entry, proliferation, and differentiation. Thus, KAI1 acts as a functional surface marker of LT-HSCs that regulates dormancy through interaction with DARC-expressing macrophages in the BM stem cell niche. Recently, we showed very special and rare macrophages expressing α-SMA+ COX2+ & DARC+ induce not only dormancy of LT-HSC through interaction of KAI1-DARC but also protect HSCs by down-regulating ROS through COX2 signaling. In the near future, the strategy to combine KAI1-positive LT-HSCs and α-SMA/Cox2/DARC triple-positive macrophages will improve the efficacy of stem cell transplantation after the ablative chemo-therapy for hematological disorders including leukemia. Korean Society for Biochemistry and Molecular Biology 2023-09-30 2023-08-23 /pmc/articles/PMC10547972/ /pubmed/37574807 http://dx.doi.org/10.5483/BMBRep.2023-0092 Text en Copyright © 2023 by the The Korean Society for Biochemistry and Molecular Biology https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Invited Mini Review
Chae, Cheong-Whan
Choi, Gun
Kim, You Ji
Cho, Mingug
Kwon, Yoo-Wook
Kim, Hyo-Soo
The maintenance mechanism of hematopoietic stem cell dormancy: role for a subset of macrophages
title The maintenance mechanism of hematopoietic stem cell dormancy: role for a subset of macrophages
title_full The maintenance mechanism of hematopoietic stem cell dormancy: role for a subset of macrophages
title_fullStr The maintenance mechanism of hematopoietic stem cell dormancy: role for a subset of macrophages
title_full_unstemmed The maintenance mechanism of hematopoietic stem cell dormancy: role for a subset of macrophages
title_short The maintenance mechanism of hematopoietic stem cell dormancy: role for a subset of macrophages
title_sort maintenance mechanism of hematopoietic stem cell dormancy: role for a subset of macrophages
topic Invited Mini Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10547972/
https://www.ncbi.nlm.nih.gov/pubmed/37574807
http://dx.doi.org/10.5483/BMBRep.2023-0092
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