Cargando…

First field and laboratory evaluation of LAMP assay for malaria diagnosis in Cubal, Angola

BACKGROUND: Malaria is a globally distributed infectious disease. According to the World Health Organization, Angola is one of the six countries that account for over half the global malaria burden in terms of both malaria cases and deaths. Diagnosis of malaria still depends on microscopic examinati...

Descripción completa

Detalles Bibliográficos
Autores principales: Febrer-Sendra, Begoña, Crego-Vicente, Beatriz, Nindia, Arlette, Martínez-Campreciós, Joan, Aixut, Sandra, Mediavilla, Alejandro, Silgado, Aroa, Oliveira-Souto, Inés, Salvador, Fernando, Molina, Israel, Muro, Antonio, Sulleiro, Elena, Fernández-Soto, Pedro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10548721/
https://www.ncbi.nlm.nih.gov/pubmed/37789462
http://dx.doi.org/10.1186/s13071-023-05942-7
_version_ 1785115332273242112
author Febrer-Sendra, Begoña
Crego-Vicente, Beatriz
Nindia, Arlette
Martínez-Campreciós, Joan
Aixut, Sandra
Mediavilla, Alejandro
Silgado, Aroa
Oliveira-Souto, Inés
Salvador, Fernando
Molina, Israel
Muro, Antonio
Sulleiro, Elena
Fernández-Soto, Pedro
author_facet Febrer-Sendra, Begoña
Crego-Vicente, Beatriz
Nindia, Arlette
Martínez-Campreciós, Joan
Aixut, Sandra
Mediavilla, Alejandro
Silgado, Aroa
Oliveira-Souto, Inés
Salvador, Fernando
Molina, Israel
Muro, Antonio
Sulleiro, Elena
Fernández-Soto, Pedro
author_sort Febrer-Sendra, Begoña
collection PubMed
description BACKGROUND: Malaria is a globally distributed infectious disease. According to the World Health Organization, Angola is one of the six countries that account for over half the global malaria burden in terms of both malaria cases and deaths. Diagnosis of malaria still depends on microscopic examination of thin and thick blood smears and rapid diagnostic tests (RDTs), which often lack analytical and clinical sensitivity. Molecular methods could overcome these disadvantages. The aim of this study was to evaluate, for the first time to our knowledge, the performance of a loop-mediated isothermal amplification (LAMP) for the diagnosis of malaria in an endemic area in Cubal, Angola, and to assess the reproducibility at a reference laboratory. METHODS: A total of 200 blood samples from patients attended at Hospital Nossa Senhora da Paz, Cubal, Angola, were analysed for Plasmodium spp. detection by microscopy, RDTs, and LAMP. LAMP assay was easily performed in a portable heating block, and the results were visualized by a simple colour change. Subsequently, the samples were sent to a reference laboratory in Spain to be reanalysed by the same colorimetric LAMP assay and also in real-time LAMP format. RESULTS: In field tests, a total of 67/200 (33.5%) blood samples were microscopy-positive for Plasmodium spp., 98/200 RDT positive, and 112/200 (56%) LAMP positive. Using microscopy as reference standard, field LAMP detected more microscopy-positive samples than RDTs (66/67; 98% vs. 62/67; 92.5%). When samples were reanalysed at a reference laboratory in Spain using both colorimetric and real-time assays, the overall reproducibility achieved 84.5%. CONCLUSIONS: This is the first study to our knowledge in which LAMP has been clinically evaluated on blood samples in a resource-poor malaria-endemic area. The colorimetric LAMP proved to be more sensitive than microscopy and RDTs for malaria diagnosis in field conditions. Furthermore, LAMP showed an acceptable level of reproducibility in a reference laboratory. The possibility to use LAMP in a real-time format in a portable device reinforces the reliability of the assay for molecular diagnosis of malaria in resource-poor laboratories in endemic areas. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-023-05942-7.
format Online
Article
Text
id pubmed-10548721
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-105487212023-10-05 First field and laboratory evaluation of LAMP assay for malaria diagnosis in Cubal, Angola Febrer-Sendra, Begoña Crego-Vicente, Beatriz Nindia, Arlette Martínez-Campreciós, Joan Aixut, Sandra Mediavilla, Alejandro Silgado, Aroa Oliveira-Souto, Inés Salvador, Fernando Molina, Israel Muro, Antonio Sulleiro, Elena Fernández-Soto, Pedro Parasit Vectors Research BACKGROUND: Malaria is a globally distributed infectious disease. According to the World Health Organization, Angola is one of the six countries that account for over half the global malaria burden in terms of both malaria cases and deaths. Diagnosis of malaria still depends on microscopic examination of thin and thick blood smears and rapid diagnostic tests (RDTs), which often lack analytical and clinical sensitivity. Molecular methods could overcome these disadvantages. The aim of this study was to evaluate, for the first time to our knowledge, the performance of a loop-mediated isothermal amplification (LAMP) for the diagnosis of malaria in an endemic area in Cubal, Angola, and to assess the reproducibility at a reference laboratory. METHODS: A total of 200 blood samples from patients attended at Hospital Nossa Senhora da Paz, Cubal, Angola, were analysed for Plasmodium spp. detection by microscopy, RDTs, and LAMP. LAMP assay was easily performed in a portable heating block, and the results were visualized by a simple colour change. Subsequently, the samples were sent to a reference laboratory in Spain to be reanalysed by the same colorimetric LAMP assay and also in real-time LAMP format. RESULTS: In field tests, a total of 67/200 (33.5%) blood samples were microscopy-positive for Plasmodium spp., 98/200 RDT positive, and 112/200 (56%) LAMP positive. Using microscopy as reference standard, field LAMP detected more microscopy-positive samples than RDTs (66/67; 98% vs. 62/67; 92.5%). When samples were reanalysed at a reference laboratory in Spain using both colorimetric and real-time assays, the overall reproducibility achieved 84.5%. CONCLUSIONS: This is the first study to our knowledge in which LAMP has been clinically evaluated on blood samples in a resource-poor malaria-endemic area. The colorimetric LAMP proved to be more sensitive than microscopy and RDTs for malaria diagnosis in field conditions. Furthermore, LAMP showed an acceptable level of reproducibility in a reference laboratory. The possibility to use LAMP in a real-time format in a portable device reinforces the reliability of the assay for molecular diagnosis of malaria in resource-poor laboratories in endemic areas. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-023-05942-7. BioMed Central 2023-10-03 /pmc/articles/PMC10548721/ /pubmed/37789462 http://dx.doi.org/10.1186/s13071-023-05942-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Febrer-Sendra, Begoña
Crego-Vicente, Beatriz
Nindia, Arlette
Martínez-Campreciós, Joan
Aixut, Sandra
Mediavilla, Alejandro
Silgado, Aroa
Oliveira-Souto, Inés
Salvador, Fernando
Molina, Israel
Muro, Antonio
Sulleiro, Elena
Fernández-Soto, Pedro
First field and laboratory evaluation of LAMP assay for malaria diagnosis in Cubal, Angola
title First field and laboratory evaluation of LAMP assay for malaria diagnosis in Cubal, Angola
title_full First field and laboratory evaluation of LAMP assay for malaria diagnosis in Cubal, Angola
title_fullStr First field and laboratory evaluation of LAMP assay for malaria diagnosis in Cubal, Angola
title_full_unstemmed First field and laboratory evaluation of LAMP assay for malaria diagnosis in Cubal, Angola
title_short First field and laboratory evaluation of LAMP assay for malaria diagnosis in Cubal, Angola
title_sort first field and laboratory evaluation of lamp assay for malaria diagnosis in cubal, angola
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10548721/
https://www.ncbi.nlm.nih.gov/pubmed/37789462
http://dx.doi.org/10.1186/s13071-023-05942-7
work_keys_str_mv AT febrersendrabegona firstfieldandlaboratoryevaluationoflampassayformalariadiagnosisincubalangola
AT cregovicentebeatriz firstfieldandlaboratoryevaluationoflampassayformalariadiagnosisincubalangola
AT nindiaarlette firstfieldandlaboratoryevaluationoflampassayformalariadiagnosisincubalangola
AT martinezcampreciosjoan firstfieldandlaboratoryevaluationoflampassayformalariadiagnosisincubalangola
AT aixutsandra firstfieldandlaboratoryevaluationoflampassayformalariadiagnosisincubalangola
AT mediavillaalejandro firstfieldandlaboratoryevaluationoflampassayformalariadiagnosisincubalangola
AT silgadoaroa firstfieldandlaboratoryevaluationoflampassayformalariadiagnosisincubalangola
AT oliveirasoutoines firstfieldandlaboratoryevaluationoflampassayformalariadiagnosisincubalangola
AT salvadorfernando firstfieldandlaboratoryevaluationoflampassayformalariadiagnosisincubalangola
AT molinaisrael firstfieldandlaboratoryevaluationoflampassayformalariadiagnosisincubalangola
AT muroantonio firstfieldandlaboratoryevaluationoflampassayformalariadiagnosisincubalangola
AT sulleiroelena firstfieldandlaboratoryevaluationoflampassayformalariadiagnosisincubalangola
AT fernandezsotopedro firstfieldandlaboratoryevaluationoflampassayformalariadiagnosisincubalangola