Biosynthesis of the fungal nonribosomal peptide penilumamide A and biochemical characterization of a pterin-specific adenylation domain

We report the characterization of the penilumamide biosynthetic cluster from Aspergillus flavipes CNL-338. In vitro reconstitution experiments demonstrated that three nonribosomal peptide synthetases are required for constructing the tripeptide and studies with dissected adenylation domains allowed...

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Detalles Bibliográficos
Autores principales: Heard, Stephanie C., Diehl, Katharine L., Winter, Jaclyn M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2023
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10549243/
https://www.ncbi.nlm.nih.gov/pubmed/37799585
http://dx.doi.org/10.1039/d3cb00088e
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author Heard, Stephanie C.
Diehl, Katharine L.
Winter, Jaclyn M.
author_facet Heard, Stephanie C.
Diehl, Katharine L.
Winter, Jaclyn M.
author_sort Heard, Stephanie C.
collection PubMed
description We report the characterization of the penilumamide biosynthetic cluster from Aspergillus flavipes CNL-338. In vitro reconstitution experiments demonstrated that three nonribosomal peptide synthetases are required for constructing the tripeptide and studies with dissected adenylation domains allowed for the first biochemical characterization of a domain that selects a pterin-derived building block.
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spelling pubmed-105492432023-10-05 Biosynthesis of the fungal nonribosomal peptide penilumamide A and biochemical characterization of a pterin-specific adenylation domain Heard, Stephanie C. Diehl, Katharine L. Winter, Jaclyn M. RSC Chem Biol Chemistry We report the characterization of the penilumamide biosynthetic cluster from Aspergillus flavipes CNL-338. In vitro reconstitution experiments demonstrated that three nonribosomal peptide synthetases are required for constructing the tripeptide and studies with dissected adenylation domains allowed for the first biochemical characterization of a domain that selects a pterin-derived building block. RSC 2023-09-06 /pmc/articles/PMC10549243/ /pubmed/37799585 http://dx.doi.org/10.1039/d3cb00088e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Heard, Stephanie C.
Diehl, Katharine L.
Winter, Jaclyn M.
Biosynthesis of the fungal nonribosomal peptide penilumamide A and biochemical characterization of a pterin-specific adenylation domain
title Biosynthesis of the fungal nonribosomal peptide penilumamide A and biochemical characterization of a pterin-specific adenylation domain
title_full Biosynthesis of the fungal nonribosomal peptide penilumamide A and biochemical characterization of a pterin-specific adenylation domain
title_fullStr Biosynthesis of the fungal nonribosomal peptide penilumamide A and biochemical characterization of a pterin-specific adenylation domain
title_full_unstemmed Biosynthesis of the fungal nonribosomal peptide penilumamide A and biochemical characterization of a pterin-specific adenylation domain
title_short Biosynthesis of the fungal nonribosomal peptide penilumamide A and biochemical characterization of a pterin-specific adenylation domain
title_sort biosynthesis of the fungal nonribosomal peptide penilumamide a and biochemical characterization of a pterin-specific adenylation domain
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10549243/
https://www.ncbi.nlm.nih.gov/pubmed/37799585
http://dx.doi.org/10.1039/d3cb00088e
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