Analysis of SARS-CoV-2 spike RBD binding to ACE2 and its inhibition by fungal cohaerin C using surface enhanced Raman spectroscopy

The structure of the SARS-CoV-2 spike RBD and human ACE2 as well as changes in the structure due to binding activities were analysed using surface enhanced Raman spectroscopy. The inhibitor cohaerin C was applied to inhibit the binding between spike RBD and ACE2. Differences and changes in the Raman...

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Detalles Bibliográficos
Autores principales: Wetzel, Christoph, Jansen-Olliges, Linda, Stadler, Marc, Surup, Frank, Zeilinger, Carsten, Roth, Bernhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optica Publishing Group 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10549735/
https://www.ncbi.nlm.nih.gov/pubmed/37799683
http://dx.doi.org/10.1364/BOE.495685
Descripción
Sumario:The structure of the SARS-CoV-2 spike RBD and human ACE2 as well as changes in the structure due to binding activities were analysed using surface enhanced Raman spectroscopy. The inhibitor cohaerin C was applied to inhibit the binding between spike RBD and ACE2. Differences and changes in the Raman spectra were determined using deconvolution of the amide bands and principal component analysis. We thus demonstrate a fast and label-free analysis of the protein structures and the differentiation between bound and unbound states. The approach is suitable for sensing and screening and might be relevant to investigate other protein systems as well.

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