Analysis of SARS-CoV-2 spike RBD binding to ACE2 and its inhibition by fungal cohaerin C using surface enhanced Raman spectroscopy

The structure of the SARS-CoV-2 spike RBD and human ACE2 as well as changes in the structure due to binding activities were analysed using surface enhanced Raman spectroscopy. The inhibitor cohaerin C was applied to inhibit the binding between spike RBD and ACE2. Differences and changes in the Raman...

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Autores principales: Wetzel, Christoph, Jansen-Olliges, Linda, Stadler, Marc, Surup, Frank, Zeilinger, Carsten, Roth, Bernhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optica Publishing Group 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10549735/
https://www.ncbi.nlm.nih.gov/pubmed/37799683
http://dx.doi.org/10.1364/BOE.495685
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author Wetzel, Christoph
Jansen-Olliges, Linda
Stadler, Marc
Surup, Frank
Zeilinger, Carsten
Roth, Bernhard
author_facet Wetzel, Christoph
Jansen-Olliges, Linda
Stadler, Marc
Surup, Frank
Zeilinger, Carsten
Roth, Bernhard
author_sort Wetzel, Christoph
collection PubMed
description The structure of the SARS-CoV-2 spike RBD and human ACE2 as well as changes in the structure due to binding activities were analysed using surface enhanced Raman spectroscopy. The inhibitor cohaerin C was applied to inhibit the binding between spike RBD and ACE2. Differences and changes in the Raman spectra were determined using deconvolution of the amide bands and principal component analysis. We thus demonstrate a fast and label-free analysis of the protein structures and the differentiation between bound and unbound states. The approach is suitable for sensing and screening and might be relevant to investigate other protein systems as well.
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spelling pubmed-105497352023-10-05 Analysis of SARS-CoV-2 spike RBD binding to ACE2 and its inhibition by fungal cohaerin C using surface enhanced Raman spectroscopy Wetzel, Christoph Jansen-Olliges, Linda Stadler, Marc Surup, Frank Zeilinger, Carsten Roth, Bernhard Biomed Opt Express Article The structure of the SARS-CoV-2 spike RBD and human ACE2 as well as changes in the structure due to binding activities were analysed using surface enhanced Raman spectroscopy. The inhibitor cohaerin C was applied to inhibit the binding between spike RBD and ACE2. Differences and changes in the Raman spectra were determined using deconvolution of the amide bands and principal component analysis. We thus demonstrate a fast and label-free analysis of the protein structures and the differentiation between bound and unbound states. The approach is suitable for sensing and screening and might be relevant to investigate other protein systems as well. Optica Publishing Group 2023-07-12 /pmc/articles/PMC10549735/ /pubmed/37799683 http://dx.doi.org/10.1364/BOE.495685 Text en Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wetzel, Christoph
Jansen-Olliges, Linda
Stadler, Marc
Surup, Frank
Zeilinger, Carsten
Roth, Bernhard
Analysis of SARS-CoV-2 spike RBD binding to ACE2 and its inhibition by fungal cohaerin C using surface enhanced Raman spectroscopy
title Analysis of SARS-CoV-2 spike RBD binding to ACE2 and its inhibition by fungal cohaerin C using surface enhanced Raman spectroscopy
title_full Analysis of SARS-CoV-2 spike RBD binding to ACE2 and its inhibition by fungal cohaerin C using surface enhanced Raman spectroscopy
title_fullStr Analysis of SARS-CoV-2 spike RBD binding to ACE2 and its inhibition by fungal cohaerin C using surface enhanced Raman spectroscopy
title_full_unstemmed Analysis of SARS-CoV-2 spike RBD binding to ACE2 and its inhibition by fungal cohaerin C using surface enhanced Raman spectroscopy
title_short Analysis of SARS-CoV-2 spike RBD binding to ACE2 and its inhibition by fungal cohaerin C using surface enhanced Raman spectroscopy
title_sort analysis of sars-cov-2 spike rbd binding to ace2 and its inhibition by fungal cohaerin c using surface enhanced raman spectroscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10549735/
https://www.ncbi.nlm.nih.gov/pubmed/37799683
http://dx.doi.org/10.1364/BOE.495685
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