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QTL mapping of oleic acid content in modern VNIIMK sunflower (Helianthus annuus L.) lines by using GBS-based SNP map
Oleic acid is a monounsaturated fatty acid increasing oil oxidative stability. High content of oleic acid is thus a valuable trait in oilseed crops. Sunflower (Helianthus annuus L.) normally accumulates linoleic acid as a major fatty acid, but a mutant expressing a high oleic phenotype form was prev...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10550128/ https://www.ncbi.nlm.nih.gov/pubmed/37792750 http://dx.doi.org/10.1371/journal.pone.0288772 |
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author | Gubaev, Rim Boldyrev, Stepan Martynova, Elena Chernova, Alina Kovalenko, Tatyana Chebanova, Yuliya Peretyagina, Tatyana Goryunova, Svetlana Goryunov, Denis Mukhina, Zhanna Ben, Cecile Gentzbittel, Laurent Khaitovich, Philipp Demurin, Yakov |
author_facet | Gubaev, Rim Boldyrev, Stepan Martynova, Elena Chernova, Alina Kovalenko, Tatyana Chebanova, Yuliya Peretyagina, Tatyana Goryunova, Svetlana Goryunov, Denis Mukhina, Zhanna Ben, Cecile Gentzbittel, Laurent Khaitovich, Philipp Demurin, Yakov |
author_sort | Gubaev, Rim |
collection | PubMed |
description | Oleic acid is a monounsaturated fatty acid increasing oil oxidative stability. High content of oleic acid is thus a valuable trait in oilseed crops. Sunflower (Helianthus annuus L.) normally accumulates linoleic acid as a major fatty acid, but a mutant expressing a high oleic phenotype form was previously obtained by chemical mutagenesis and mapped on the sunflower genome. Several studies suggest the presence of additional genes involved in the control of the high content of oleic acid, with their expression possibly depending on the genetic background. To test this hypothesis, we performed a QTL mapping of the high oleic acid trait within two independent F(2) crosses involving lines with contrasting oleic acid content from the Pustovoit All-Russia Research Institute of Oil Crops (VNIIMK) collection. We applied genotyping-by-sequencing (GBS) to construct single nucleotide polymorphism-based genetic maps and performed QTL mapping using quantitative and qualitative encoding for oleic acid content. Our results support the finding that the oleic acid content in the assessed crosses is controlled by one major effect locus. However, different dominant/recessive effects of the major locus were reported for both crosses. Additionally, a possible translocation between chromosome 7 and 14 was reported in one assessed cross. We defined a set of single nucleotide polymorphism markers for each cross which could be used for marker-assisted selection. |
format | Online Article Text |
id | pubmed-10550128 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-105501282023-10-05 QTL mapping of oleic acid content in modern VNIIMK sunflower (Helianthus annuus L.) lines by using GBS-based SNP map Gubaev, Rim Boldyrev, Stepan Martynova, Elena Chernova, Alina Kovalenko, Tatyana Chebanova, Yuliya Peretyagina, Tatyana Goryunova, Svetlana Goryunov, Denis Mukhina, Zhanna Ben, Cecile Gentzbittel, Laurent Khaitovich, Philipp Demurin, Yakov PLoS One Research Article Oleic acid is a monounsaturated fatty acid increasing oil oxidative stability. High content of oleic acid is thus a valuable trait in oilseed crops. Sunflower (Helianthus annuus L.) normally accumulates linoleic acid as a major fatty acid, but a mutant expressing a high oleic phenotype form was previously obtained by chemical mutagenesis and mapped on the sunflower genome. Several studies suggest the presence of additional genes involved in the control of the high content of oleic acid, with their expression possibly depending on the genetic background. To test this hypothesis, we performed a QTL mapping of the high oleic acid trait within two independent F(2) crosses involving lines with contrasting oleic acid content from the Pustovoit All-Russia Research Institute of Oil Crops (VNIIMK) collection. We applied genotyping-by-sequencing (GBS) to construct single nucleotide polymorphism-based genetic maps and performed QTL mapping using quantitative and qualitative encoding for oleic acid content. Our results support the finding that the oleic acid content in the assessed crosses is controlled by one major effect locus. However, different dominant/recessive effects of the major locus were reported for both crosses. Additionally, a possible translocation between chromosome 7 and 14 was reported in one assessed cross. We defined a set of single nucleotide polymorphism markers for each cross which could be used for marker-assisted selection. Public Library of Science 2023-10-04 /pmc/articles/PMC10550128/ /pubmed/37792750 http://dx.doi.org/10.1371/journal.pone.0288772 Text en © 2023 Gubaev et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Gubaev, Rim Boldyrev, Stepan Martynova, Elena Chernova, Alina Kovalenko, Tatyana Chebanova, Yuliya Peretyagina, Tatyana Goryunova, Svetlana Goryunov, Denis Mukhina, Zhanna Ben, Cecile Gentzbittel, Laurent Khaitovich, Philipp Demurin, Yakov QTL mapping of oleic acid content in modern VNIIMK sunflower (Helianthus annuus L.) lines by using GBS-based SNP map |
title | QTL mapping of oleic acid content in modern VNIIMK sunflower (Helianthus annuus L.) lines by using GBS-based SNP map |
title_full | QTL mapping of oleic acid content in modern VNIIMK sunflower (Helianthus annuus L.) lines by using GBS-based SNP map |
title_fullStr | QTL mapping of oleic acid content in modern VNIIMK sunflower (Helianthus annuus L.) lines by using GBS-based SNP map |
title_full_unstemmed | QTL mapping of oleic acid content in modern VNIIMK sunflower (Helianthus annuus L.) lines by using GBS-based SNP map |
title_short | QTL mapping of oleic acid content in modern VNIIMK sunflower (Helianthus annuus L.) lines by using GBS-based SNP map |
title_sort | qtl mapping of oleic acid content in modern vniimk sunflower (helianthus annuus l.) lines by using gbs-based snp map |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10550128/ https://www.ncbi.nlm.nih.gov/pubmed/37792750 http://dx.doi.org/10.1371/journal.pone.0288772 |
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