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Resveratrol Attenuates Degeneration and Apoptosis of Cardiomyocytes Induced by Aortic Clamping
INTRODUCTION: Objective: To investigate the potential beneficial effects of resveratrol (RVT) against ischemia-reperfusion injury of myocardial tissue during surgical treatment of ruptured abdominal aortic aneurysm. METHODS: Four groups were established — control, ischemia/reperfusion (I/R), sham (I...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Cirurgia Cardiovascular
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10550301/ https://www.ncbi.nlm.nih.gov/pubmed/37801567 http://dx.doi.org/10.21470/1678-9741-2023-0224 |
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author | Ergene, Saban Hemsinli, Dogus Karakisi, Sedat Ozan Tümkaya, Levent Mercantepe, Tolga Yilmaz, Adnan Yel, Ibrahim |
author_facet | Ergene, Saban Hemsinli, Dogus Karakisi, Sedat Ozan Tümkaya, Levent Mercantepe, Tolga Yilmaz, Adnan Yel, Ibrahim |
author_sort | Ergene, Saban |
collection | PubMed |
description | INTRODUCTION: Objective: To investigate the potential beneficial effects of resveratrol (RVT) against ischemia-reperfusion injury of myocardial tissue during surgical treatment of ruptured abdominal aortic aneurysm. METHODS: Four groups were established — control, ischemia/reperfusion (I/R), sham (I/R+solvent/dimethyl sulfoxide [(DMSO)]), and I/R+RVT. Ruptured abdominal aortic aneurysm model was used as the experimental protocol. RESULTS: In the I/R and I/R+DMSO groups, malondialdehyde (MDA) levels in myocardial tissue were found to be significantly increased compared to the control group. The MDA level in myocardial tissue was significantly decreased in the I/ R+RVT group compared to the I/R group. In I/R and I/R+DMSO groups, glutathione peroxidase (GSH) levels in myocardial tissue were found to be significantly decreased compared to the control group. The GSH level in the myocardial tissue was significantly increased in the I/R+RVT group compared to the I/R group. In the light microscope, isotropic and anisotropic band disorganized atypical cardiomyocytes in the I/R group and degenerative cardiomyocytes and edematous areas in the I/R+DMSO group were observed. Degenerative cardiomyocytes and edematous areas were decreased in the I/R+RVT group. When heart tissue sections incubated with cleaved caspase-3 primary antibodies were examined under the light microscope, apoptotic cardiomyocytes were present in I/R and I/R+DMSO groups. A decrease in the number of apoptotic cardiomyocytes was observed in the I/R+RVT group. CONCLUSION: The findings of the present study indicate that RVT exhibits protective effects against ischemia-reperfusion injury occurring in the myocardium as a distant organ as a result of abdominal aorta clamping. |
format | Online Article Text |
id | pubmed-10550301 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Sociedade Brasileira de Cirurgia Cardiovascular |
record_format | MEDLINE/PubMed |
spelling | pubmed-105503012023-10-05 Resveratrol Attenuates Degeneration and Apoptosis of Cardiomyocytes Induced by Aortic Clamping Ergene, Saban Hemsinli, Dogus Karakisi, Sedat Ozan Tümkaya, Levent Mercantepe, Tolga Yilmaz, Adnan Yel, Ibrahim Braz J Cardiovasc Surg Original Article INTRODUCTION: Objective: To investigate the potential beneficial effects of resveratrol (RVT) against ischemia-reperfusion injury of myocardial tissue during surgical treatment of ruptured abdominal aortic aneurysm. METHODS: Four groups were established — control, ischemia/reperfusion (I/R), sham (I/R+solvent/dimethyl sulfoxide [(DMSO)]), and I/R+RVT. Ruptured abdominal aortic aneurysm model was used as the experimental protocol. RESULTS: In the I/R and I/R+DMSO groups, malondialdehyde (MDA) levels in myocardial tissue were found to be significantly increased compared to the control group. The MDA level in myocardial tissue was significantly decreased in the I/ R+RVT group compared to the I/R group. In I/R and I/R+DMSO groups, glutathione peroxidase (GSH) levels in myocardial tissue were found to be significantly decreased compared to the control group. The GSH level in the myocardial tissue was significantly increased in the I/R+RVT group compared to the I/R group. In the light microscope, isotropic and anisotropic band disorganized atypical cardiomyocytes in the I/R group and degenerative cardiomyocytes and edematous areas in the I/R+DMSO group were observed. Degenerative cardiomyocytes and edematous areas were decreased in the I/R+RVT group. When heart tissue sections incubated with cleaved caspase-3 primary antibodies were examined under the light microscope, apoptotic cardiomyocytes were present in I/R and I/R+DMSO groups. A decrease in the number of apoptotic cardiomyocytes was observed in the I/R+RVT group. CONCLUSION: The findings of the present study indicate that RVT exhibits protective effects against ischemia-reperfusion injury occurring in the myocardium as a distant organ as a result of abdominal aorta clamping. Sociedade Brasileira de Cirurgia Cardiovascular 2023-09-19 /pmc/articles/PMC10550301/ /pubmed/37801567 http://dx.doi.org/10.21470/1678-9741-2023-0224 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Ergene, Saban Hemsinli, Dogus Karakisi, Sedat Ozan Tümkaya, Levent Mercantepe, Tolga Yilmaz, Adnan Yel, Ibrahim Resveratrol Attenuates Degeneration and Apoptosis of Cardiomyocytes Induced by Aortic Clamping |
title | Resveratrol Attenuates Degeneration and Apoptosis of Cardiomyocytes
Induced by Aortic Clamping |
title_full | Resveratrol Attenuates Degeneration and Apoptosis of Cardiomyocytes
Induced by Aortic Clamping |
title_fullStr | Resveratrol Attenuates Degeneration and Apoptosis of Cardiomyocytes
Induced by Aortic Clamping |
title_full_unstemmed | Resveratrol Attenuates Degeneration and Apoptosis of Cardiomyocytes
Induced by Aortic Clamping |
title_short | Resveratrol Attenuates Degeneration and Apoptosis of Cardiomyocytes
Induced by Aortic Clamping |
title_sort | resveratrol attenuates degeneration and apoptosis of cardiomyocytes
induced by aortic clamping |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10550301/ https://www.ncbi.nlm.nih.gov/pubmed/37801567 http://dx.doi.org/10.21470/1678-9741-2023-0224 |
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