Effect of carrier yeast RNAs in the detection of SARS-CoV-2 by RT-LAMP

The COVID-19 pandemic caused by SARS-CoV-2 has underscored the need for rapid and accurate diagnostic methods. Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) has emerged as a promising molecular tool in least developed countries due to its simplicity, speed, and sensitivity....

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Detalles Bibliográficos
Autores principales: Morais-Armas, Samantha, Medina-Suárez, Sara, Machín, Félix
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10550381/
https://www.ncbi.nlm.nih.gov/pubmed/37799204
http://dx.doi.org/10.17912/micropub.biology.000979
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author Morais-Armas, Samantha
Medina-Suárez, Sara
Machín, Félix
author_facet Morais-Armas, Samantha
Medina-Suárez, Sara
Machín, Félix
author_sort Morais-Armas, Samantha
collection PubMed
description The COVID-19 pandemic caused by SARS-CoV-2 has underscored the need for rapid and accurate diagnostic methods. Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) has emerged as a promising molecular tool in least developed countries due to its simplicity, speed, and sensitivity. Nevertheless, reliable SARS-CoV-2 detection can be challenged by the chain custody of the samples. In this context, carrier RNA can act as a preservative. In this study, we explored the potential of yeast total and transference RNA (tRNA) in the SARS-CoV-2 RT-LAMP. We have found that most optimal conditions are reached with 1 μg/μL tRNA in the RT-LAMP reaction.
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spelling pubmed-105503812023-10-05 Effect of carrier yeast RNAs in the detection of SARS-CoV-2 by RT-LAMP Morais-Armas, Samantha Medina-Suárez, Sara Machín, Félix MicroPubl Biol New Finding The COVID-19 pandemic caused by SARS-CoV-2 has underscored the need for rapid and accurate diagnostic methods. Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) has emerged as a promising molecular tool in least developed countries due to its simplicity, speed, and sensitivity. Nevertheless, reliable SARS-CoV-2 detection can be challenged by the chain custody of the samples. In this context, carrier RNA can act as a preservative. In this study, we explored the potential of yeast total and transference RNA (tRNA) in the SARS-CoV-2 RT-LAMP. We have found that most optimal conditions are reached with 1 μg/μL tRNA in the RT-LAMP reaction. Caltech Library 2023-09-20 /pmc/articles/PMC10550381/ /pubmed/37799204 http://dx.doi.org/10.17912/micropub.biology.000979 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Morais-Armas, Samantha
Medina-Suárez, Sara
Machín, Félix
Effect of carrier yeast RNAs in the detection of SARS-CoV-2 by RT-LAMP
title Effect of carrier yeast RNAs in the detection of SARS-CoV-2 by RT-LAMP
title_full Effect of carrier yeast RNAs in the detection of SARS-CoV-2 by RT-LAMP
title_fullStr Effect of carrier yeast RNAs in the detection of SARS-CoV-2 by RT-LAMP
title_full_unstemmed Effect of carrier yeast RNAs in the detection of SARS-CoV-2 by RT-LAMP
title_short Effect of carrier yeast RNAs in the detection of SARS-CoV-2 by RT-LAMP
title_sort effect of carrier yeast rnas in the detection of sars-cov-2 by rt-lamp
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10550381/
https://www.ncbi.nlm.nih.gov/pubmed/37799204
http://dx.doi.org/10.17912/micropub.biology.000979
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