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Oral fungal profiling and risk of nasopharyngeal carcinoma: a population-based case-control study

BACKGROUND: Dysbiosis of the oral mycobiome has been linked to some diseases, including cancers. However, the role of oral fungal communities in nasopharyngeal carcinoma (NPC) carcinogenesis has not previously been investigated. METHODS: We characterized the oral salivary fungal mycobiome in 476 unt...

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Autores principales: Chen, Yufeng, Li, Wanxin, Chang, Ellen T., Debelius, Justine W., Manoharan, Lokeshwaran, Zheng, Yuming, Li, Yancheng, Huang, Guangwu, Adami, Hans-Olov, Knight, Rob, Cai, Yonglin, Zhang, Zhe, Ye, Weimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10550808/
https://www.ncbi.nlm.nih.gov/pubmed/37776725
http://dx.doi.org/10.1016/j.ebiom.2023.104813
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author Chen, Yufeng
Li, Wanxin
Chang, Ellen T.
Debelius, Justine W.
Manoharan, Lokeshwaran
Zheng, Yuming
Li, Yancheng
Huang, Guangwu
Adami, Hans-Olov
Knight, Rob
Cai, Yonglin
Zhang, Zhe
Ye, Weimin
author_facet Chen, Yufeng
Li, Wanxin
Chang, Ellen T.
Debelius, Justine W.
Manoharan, Lokeshwaran
Zheng, Yuming
Li, Yancheng
Huang, Guangwu
Adami, Hans-Olov
Knight, Rob
Cai, Yonglin
Zhang, Zhe
Ye, Weimin
author_sort Chen, Yufeng
collection PubMed
description BACKGROUND: Dysbiosis of the oral mycobiome has been linked to some diseases, including cancers. However, the role of oral fungal communities in nasopharyngeal carcinoma (NPC) carcinogenesis has not previously been investigated. METHODS: We characterized the oral salivary fungal mycobiome in 476 untreated incident NPC patients and 537 population-based controls using fungal internal transcribed spacer (ITS)-2 sequencing. The relationship between oral fungal mycobiome and the risk of NPC was assessed through bioinformatic and biostatistical analyses. FINDINGS: We found that lower fungal alpha diversity was associated with an increased odds of NPC [lower vs. higher: observed features (adjusted odds ratio [OR] = 5.81, 95% confidence interval [CI] = 3.60–9.38); Simpson diversity (1.53, 1.03–2.29); Shannon diversity (2.03, 1.35–3.04)]. We also observed a significant difference in global fungal community patterns between cases and controls based on Bray–Curtis dissimilarity (P < 0.001). Carriage of oral fungal species, specifically, Saccharomyces cerevisiae, Candida tropicalis, Lodderomyces elongisporus, Candida albicans, and Fusarium poae, was associated with significantly higher odds of NPC, with ORs ranging from 1.56 to 4.66. Individuals with both low fungal and low bacterial alpha diversity had a profoundly elevated risk of NPC. INTERPRETATION: Our results suggest that dysbiosis in the oral mycobiome, characterized by a loss of fungal community diversity and overgrowth of several fungal organisms, is associated with a substantially increased risk of NPC. FUNDING: This work was funded by the 10.13039/100000002US National Institutes of Health, the 10.13039/501100004359Swedish Research Council, the High-level Talents Research Start-up Project of Fujian Medical University, and the 10.13039/501100004543China Scholarship Council.
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spelling pubmed-105508082023-10-06 Oral fungal profiling and risk of nasopharyngeal carcinoma: a population-based case-control study Chen, Yufeng Li, Wanxin Chang, Ellen T. Debelius, Justine W. Manoharan, Lokeshwaran Zheng, Yuming Li, Yancheng Huang, Guangwu Adami, Hans-Olov Knight, Rob Cai, Yonglin Zhang, Zhe Ye, Weimin eBioMedicine Articles BACKGROUND: Dysbiosis of the oral mycobiome has been linked to some diseases, including cancers. However, the role of oral fungal communities in nasopharyngeal carcinoma (NPC) carcinogenesis has not previously been investigated. METHODS: We characterized the oral salivary fungal mycobiome in 476 untreated incident NPC patients and 537 population-based controls using fungal internal transcribed spacer (ITS)-2 sequencing. The relationship between oral fungal mycobiome and the risk of NPC was assessed through bioinformatic and biostatistical analyses. FINDINGS: We found that lower fungal alpha diversity was associated with an increased odds of NPC [lower vs. higher: observed features (adjusted odds ratio [OR] = 5.81, 95% confidence interval [CI] = 3.60–9.38); Simpson diversity (1.53, 1.03–2.29); Shannon diversity (2.03, 1.35–3.04)]. We also observed a significant difference in global fungal community patterns between cases and controls based on Bray–Curtis dissimilarity (P < 0.001). Carriage of oral fungal species, specifically, Saccharomyces cerevisiae, Candida tropicalis, Lodderomyces elongisporus, Candida albicans, and Fusarium poae, was associated with significantly higher odds of NPC, with ORs ranging from 1.56 to 4.66. Individuals with both low fungal and low bacterial alpha diversity had a profoundly elevated risk of NPC. INTERPRETATION: Our results suggest that dysbiosis in the oral mycobiome, characterized by a loss of fungal community diversity and overgrowth of several fungal organisms, is associated with a substantially increased risk of NPC. FUNDING: This work was funded by the 10.13039/100000002US National Institutes of Health, the 10.13039/501100004359Swedish Research Council, the High-level Talents Research Start-up Project of Fujian Medical University, and the 10.13039/501100004543China Scholarship Council. Elsevier 2023-09-28 /pmc/articles/PMC10550808/ /pubmed/37776725 http://dx.doi.org/10.1016/j.ebiom.2023.104813 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Articles
Chen, Yufeng
Li, Wanxin
Chang, Ellen T.
Debelius, Justine W.
Manoharan, Lokeshwaran
Zheng, Yuming
Li, Yancheng
Huang, Guangwu
Adami, Hans-Olov
Knight, Rob
Cai, Yonglin
Zhang, Zhe
Ye, Weimin
Oral fungal profiling and risk of nasopharyngeal carcinoma: a population-based case-control study
title Oral fungal profiling and risk of nasopharyngeal carcinoma: a population-based case-control study
title_full Oral fungal profiling and risk of nasopharyngeal carcinoma: a population-based case-control study
title_fullStr Oral fungal profiling and risk of nasopharyngeal carcinoma: a population-based case-control study
title_full_unstemmed Oral fungal profiling and risk of nasopharyngeal carcinoma: a population-based case-control study
title_short Oral fungal profiling and risk of nasopharyngeal carcinoma: a population-based case-control study
title_sort oral fungal profiling and risk of nasopharyngeal carcinoma: a population-based case-control study
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10550808/
https://www.ncbi.nlm.nih.gov/pubmed/37776725
http://dx.doi.org/10.1016/j.ebiom.2023.104813
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