Cargando…

Purification and functional validation of LtCas12a protein

Here, we present a protocol for generating LtCas12a protein recognizing distinct TTNA (N represented A, T, C, G) protospacer adjacent motif sequence. We describe steps for transforming and harvesting bacterial cells and protein purification including nickel affinity chromatography and dialysis. We t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zhou, Bo, Chen, Ye, Li, Lifang, Liu, Jiashuo, Wang, Yuyan, Huang, Zheying, Hu, Zheng, Tian, Rui, Li, Zhen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10550837/ https://www.ncbi.nlm.nih.gov/pubmed/37768827 http://dx.doi.org/10.1016/j.xpro.2023.102600

Ejemplares similares

A Cas12a ortholog with distinct TTNA PAM enables sensitive detection of HPV16/18
por: Chen, Ye, et al.
Publicado: (2023)

FrCas9 is a CRISPR/Cas9 system with high editing efficiency and fidelity
por: Cui, Zifeng, et al.
Publicado: (2022)

VIS Atlas: A Database of Virus Integration Sites in Human Genome from NGS Data to Explore Integration Patterns
por: Chen, Ye, et al.
Publicado: (2023)

An efficient and cost-effective purification protocol for Staphylococcus aureus Cas9 nuclease
por: Teng, Allen C.T., et al.
Publicado: (2022)

A detailed protocol for expression, purification, and activity determination of recombinant SaCas9
por: Flottmann, Franziska, et al.
Publicado: (2022)

HPV integration generates a cellular super-enhancer which functions as ecDNA to regulate genome-wide transcription
por: Tian, Rui, et al.
Publicado: (2023)

Gene knock-out chain reaction enables high disruption efficiency of HPV18 E6/E7 genes in cervical cancer cells
por: Tian, Rui, et al.
Publicado: (2021)

Whole-Genome Shotgun Sequences of Salmonella enterica Serovar Typhimurium Lilleengen Type Strains LT1, LT18, LT19, LT20, LT21, and LT22
por: Kazmierczak, Robert A., et al.
Publicado: (2017)

A Robust Expression and Purification Method for Production of SpCas9-GFP-MBP Fusion Protein for In Vitro Applications
por: Fleitas, Andrea Luciana, et al.
Publicado: (2022)

The comparison of ZFNs, TALENs, and SpCas9 by GUIDE-seq in HPV-targeted gene therapy
por: Cui, Zifeng, et al.
Publicado: (2021)

IL-12 supports in vitro self-renewal of LT HSC
Publicado: (2022)

Expanding the genome editing toolbox of Saccharomyces cerevisiae with the endonuclease ErCas12a
por: Bennis, Nicole X, et al.
Publicado: (2023)

Purification and cryoelectron microscopy structure determination of human V-ATPase
por: Wang, Longfei, et al.
Publicado: (2021)

Efficient generation of locus-specific human CAR-T cells with CRISPR/cCas12a
por: Ling, Xinyu, et al.
Publicado: (2022)

An in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragments
por: Zeng, Xiaoqian, et al.
Publicado: (2023)

Purification of hetero-oligomeric protein variants using a modified tandem affinity purification approach
por: Parui, Aasna L., et al.
Publicado: (2023)

Production and purification of infectious diseases-associated tetramer for staining of CD8(+) T cells from PBMCs
por: Ye, Jiezhou, et al.
Publicado: (2022)

VALIDATION OF THE HADRONIC CALIBRATION OF THE ATLAS CALORIMETER WITH TESTBEAM DATA CORRESPONDING TO THE PSEUDORAPIDITY RANGE $2.5<|eta|<4.0$}
por: Pospelov, G
Publicado: (2009)

Validation of the hadronic calibration of the ATLAS calorimeter with testbeam data corresponding to the pseudorapidity range 2.5<|eta|<4.0
por: Pospelov, G
Publicado: (2009)

CRISPR-Cas12a ribonucleoprotein-mediated gene editing in the plant pathogenic fungus Magnaporthe oryzae
por: Huang, Jun, et al.
Publicado: (2021)

Lt.-Col. Maynard
Publicado: (1921)

Purification of Recombinant Galectins Expressed in Bacteria
por: Prato, Cecilia Arahí, et al.
Publicado: (2020)

The LT1 and LT2 variants of the enterotoxigenic Escherichia coli (ETEC) heat-labile toxin (LT) are associated with major ETEC lineages
por: Joffré, Enrique, et al.
Publicado: (2016)

Designing a combined liothyronine (LT3), L- thyroxine (LT4) trial in symptomatic hypothyroid subjects on LT4 - the importance of patient selection, choice of LT3 and trial design
por: Premawardhana, Lakdasa D., et al.
Publicado: (2023)

Assembly and purification of AvrSr35-induced Sr35 resistosome and determination of its structure by cryo-EM
por: Liu, Meng-Xi, et al.
Publicado: (2022)

A multi-center cross-sectional study on blood purification among adult patients in intensive care unit in China: a study protocol
por: Gao, Yang, et al.
Publicado: (2019)

Net widens for funding of <i>arXiv</i>
Publicado: (2010)

Ligamentum Teres (LT) Rupture: Safety And Efficacy Of Allograft And Synthetic Ligament In LT Reconstruction
por: Bajwa, Ali S., et al.
Publicado: (2016)

Expression and purification of phage T7 ejection proteins for cryo-EM analysis
por: Swanson, Nicholas A., et al.
Publicado: (2021)

Protocol for high-throughput cloning, expression, purification, and evaluation of bispecific antibodies
por: Li, Danqing, et al.
Publicado: (2022)

Purification and quantitative proteomic analysis of cell bodies and protrusions
por: Dermit, Maria, et al.
Publicado: (2021)

Purification of micronuclei from cultured cells by flow cytometry
por: Toufektchan, Eléonore, et al.
Publicado: (2021)

Lt.-Col. Robert Pringle
Publicado: (1899)

Reply letter to Leong LT
por: Girometti, Rossano, et al.
Publicado: (2020)

Lt. Martin F. Nolan
Publicado: (1918)

Expression of cysLT1 and cysLT2 Receptor in Chronic Hyperplastic Eosinophilic Sinusitis
por: Ouyang, Yuhui, et al.
Publicado: (2009)

<em>Reg</em> reader unlocks secrets of LHC restart
Publicado: (2009)

The Mucosal Vaccine Adjuvant LT(R192G/L211A) or dmLT
por: Clements, John D., et al.
Publicado: (2018)

Efficient purification and assembly of ribonucleoprotein complex for interaction analysis by MST assay coupled with GaMD simulations
por: Gao, Yunrong, et al.
Publicado: (2021)

Improved Method for DNA Extraction and Purification from Tetrahymena pyriformis
por: El Maaiden, Ezzouhra, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_r9tb5r78qjidnjshp2480furn4