PAM-flexible genome editing with an engineered chimeric Cas9

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CRISPR enzymes require a defined protospacer adjacent motif (PAM) flanking a guide RNA-programmed target site, limiting their sequence accessibility for robust genome editing applications. In this study, we recombine the PAM-interacting domain of SpRY, a broad-targeting Cas9 possessing an NRN > N...

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Detalles Bibliográficos
Autores principales: Zhao, Lin, Koseki, Sabrina R. T., Silverstein, Rachel A., Amrani, Nadia, Peng, Christina, Kramme, Christian, Savic, Natasha, Pacesa, Martin, Rodríguez, Tomás C., Stan, Teodora, Tysinger, Emma, Hong, Lauren, Yudistyra, Vivian, Ponnapati, Manvitha R., Jacobson, Joseph M., Church, George M., Jakimo, Noah, Truant, Ray, Jinek, Martin, Kleinstiver, Benjamin P., Sontheimer, Erik J., Chatterjee, Pranam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10550912/
https://www.ncbi.nlm.nih.gov/pubmed/37794046
http://dx.doi.org/10.1038/s41467-023-41829-y

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