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Macrophage-mediated trogocytosis contributes to destroying human schistosomes in a non-susceptible rodent host, Microtus fortis
Schistosoma parasites, causing schistosomiasis, exhibit typical host specificity in host preference. Many mammals, including humans, are susceptible to infection, while the widely distributed rodent, Microtus fortis, exhibits natural anti-schistosome characteristics. The mechanisms of host susceptib...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Nature Singapore
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10550985/ https://www.ncbi.nlm.nih.gov/pubmed/37794085 http://dx.doi.org/10.1038/s41421-023-00603-6 |
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author | Shen, Jia Zhao, Siyu Peng, Mei Li, Yanguo Zhang, Lichao Li, Xiaoping Hu, Yunyi Wu, Mingrou Xiang, Suoyu Wu, Xiaoying Liu, Jiahua Zhang, Beibei Chen, Zebin Lin, Datao Liu, Huanyao Tang, Wenyan Chen, Jun Sun, Xi Liao, Qi Hide, Geoff Zhou, Zhijun Lun, Zhao-Rong Wu, Zhongdao |
author_facet | Shen, Jia Zhao, Siyu Peng, Mei Li, Yanguo Zhang, Lichao Li, Xiaoping Hu, Yunyi Wu, Mingrou Xiang, Suoyu Wu, Xiaoying Liu, Jiahua Zhang, Beibei Chen, Zebin Lin, Datao Liu, Huanyao Tang, Wenyan Chen, Jun Sun, Xi Liao, Qi Hide, Geoff Zhou, Zhijun Lun, Zhao-Rong Wu, Zhongdao |
author_sort | Shen, Jia |
collection | PubMed |
description | Schistosoma parasites, causing schistosomiasis, exhibit typical host specificity in host preference. Many mammals, including humans, are susceptible to infection, while the widely distributed rodent, Microtus fortis, exhibits natural anti-schistosome characteristics. The mechanisms of host susceptibility remain poorly understood. Comparison of schistosome infection in M. fortis with the infection in laboratory mice (highly sensitive to infection) offers a good model system to investigate these mechanisms and to gain an insight into host specificity. In this study, we showed that large numbers of leukocytes attach to the surface of human schistosomes in M. fortis but not in mice. Single-cell RNA-sequencing analyses revealed that macrophages might be involved in the cell adhesion, and we further demonstrated that M. fortis macrophages could be mediated to attach and kill schistosomula with dependence on Complement component 3 (C3) and Complement receptor 3 (CR3). Importantly, we provided direct evidence that M. fortis macrophages could destroy schistosomula by trogocytosis, a previously undescribed mode for killing helminths. This process was regulated by Ca(2+)/NFAT signaling. These findings not only elucidate a novel anti-schistosome mechanism in M. fortis but also provide a better understanding of host parasite interactions, host specificity and the potential generation of novel strategies for schistosomiasis control. |
format | Online Article Text |
id | pubmed-10550985 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Nature Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-105509852023-10-06 Macrophage-mediated trogocytosis contributes to destroying human schistosomes in a non-susceptible rodent host, Microtus fortis Shen, Jia Zhao, Siyu Peng, Mei Li, Yanguo Zhang, Lichao Li, Xiaoping Hu, Yunyi Wu, Mingrou Xiang, Suoyu Wu, Xiaoying Liu, Jiahua Zhang, Beibei Chen, Zebin Lin, Datao Liu, Huanyao Tang, Wenyan Chen, Jun Sun, Xi Liao, Qi Hide, Geoff Zhou, Zhijun Lun, Zhao-Rong Wu, Zhongdao Cell Discov Article Schistosoma parasites, causing schistosomiasis, exhibit typical host specificity in host preference. Many mammals, including humans, are susceptible to infection, while the widely distributed rodent, Microtus fortis, exhibits natural anti-schistosome characteristics. The mechanisms of host susceptibility remain poorly understood. Comparison of schistosome infection in M. fortis with the infection in laboratory mice (highly sensitive to infection) offers a good model system to investigate these mechanisms and to gain an insight into host specificity. In this study, we showed that large numbers of leukocytes attach to the surface of human schistosomes in M. fortis but not in mice. Single-cell RNA-sequencing analyses revealed that macrophages might be involved in the cell adhesion, and we further demonstrated that M. fortis macrophages could be mediated to attach and kill schistosomula with dependence on Complement component 3 (C3) and Complement receptor 3 (CR3). Importantly, we provided direct evidence that M. fortis macrophages could destroy schistosomula by trogocytosis, a previously undescribed mode for killing helminths. This process was regulated by Ca(2+)/NFAT signaling. These findings not only elucidate a novel anti-schistosome mechanism in M. fortis but also provide a better understanding of host parasite interactions, host specificity and the potential generation of novel strategies for schistosomiasis control. Springer Nature Singapore 2023-10-05 /pmc/articles/PMC10550985/ /pubmed/37794085 http://dx.doi.org/10.1038/s41421-023-00603-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Shen, Jia Zhao, Siyu Peng, Mei Li, Yanguo Zhang, Lichao Li, Xiaoping Hu, Yunyi Wu, Mingrou Xiang, Suoyu Wu, Xiaoying Liu, Jiahua Zhang, Beibei Chen, Zebin Lin, Datao Liu, Huanyao Tang, Wenyan Chen, Jun Sun, Xi Liao, Qi Hide, Geoff Zhou, Zhijun Lun, Zhao-Rong Wu, Zhongdao Macrophage-mediated trogocytosis contributes to destroying human schistosomes in a non-susceptible rodent host, Microtus fortis |
title | Macrophage-mediated trogocytosis contributes to destroying human schistosomes in a non-susceptible rodent host, Microtus fortis |
title_full | Macrophage-mediated trogocytosis contributes to destroying human schistosomes in a non-susceptible rodent host, Microtus fortis |
title_fullStr | Macrophage-mediated trogocytosis contributes to destroying human schistosomes in a non-susceptible rodent host, Microtus fortis |
title_full_unstemmed | Macrophage-mediated trogocytosis contributes to destroying human schistosomes in a non-susceptible rodent host, Microtus fortis |
title_short | Macrophage-mediated trogocytosis contributes to destroying human schistosomes in a non-susceptible rodent host, Microtus fortis |
title_sort | macrophage-mediated trogocytosis contributes to destroying human schistosomes in a non-susceptible rodent host, microtus fortis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10550985/ https://www.ncbi.nlm.nih.gov/pubmed/37794085 http://dx.doi.org/10.1038/s41421-023-00603-6 |
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