Evaluation of guide-free Cas9-induced genomic damage and transcriptome changes in pig embryos

Cas9 protein without sgRNAs can induce genomic damage at the cellular level in vitro. However, whether the detrimental effects occur in embryos after Cas9 treatment remains unknown. Here, using pig embryos as subjects, we observed that Cas9 protein transcribed from injected Cas9 mRNA can persist unt...

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Detalles Bibliográficos
Autores principales: Ge, Weikai, Zhao, Xiaozhu, Gou, Shixue, Jin, Qin, Chen, Fangbing, Ouyang, Zhen, Lai, Chengdan, Cui, Tao, Mai, Baoyi, Lu, Sijia, Zhong, Kexin, Liang, Yanhui, Chen, Tao, Wu, Han, Li, Nan, Ye, Yinghua, Lai, Liangxue, Wang, Kepin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2023
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10551558/
https://www.ncbi.nlm.nih.gov/pubmed/37808924
http://dx.doi.org/10.1016/j.omtn.2023.102035

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10551558/
https://www.ncbi.nlm.nih.gov/pubmed/37808924
http://dx.doi.org/10.1016/j.omtn.2023.102035

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