Protocol to investigate the neural basis for copulation posture of Drosophila using a closed-loop real-time optogenetic system

In internal fertilization animals, maintaining a copulation posture facilitates the process of transporting gametes from male to female. Here, we present a protocol to investigate the neural basis for copulation posture of fruit flies using a closed-loop real-time optogenetic system. We describe ste...

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Detalles Bibliográficos
Autores principales: Yamanouchi, Hayato M., Kamikouchi, Azusa, Tanaka, Ryoya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10551656/
https://www.ncbi.nlm.nih.gov/pubmed/37788165
http://dx.doi.org/10.1016/j.xpro.2023.102623
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author Yamanouchi, Hayato M.
Kamikouchi, Azusa
Tanaka, Ryoya
author_facet Yamanouchi, Hayato M.
Kamikouchi, Azusa
Tanaka, Ryoya
author_sort Yamanouchi, Hayato M.
collection PubMed
description In internal fertilization animals, maintaining a copulation posture facilitates the process of transporting gametes from male to female. Here, we present a protocol to investigate the neural basis for copulation posture of fruit flies using a closed-loop real-time optogenetic system. We describe steps for using deep learning analysis to enable optogenetic manipulation of neural activity only during copulation with high efficiency. This system can be applied to various animal behaviors other than copulation. For complete details on the use and execution of this protocol, please refer to Yamanouchi et al. (2023).(1)
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spelling pubmed-105516562023-10-06 Protocol to investigate the neural basis for copulation posture of Drosophila using a closed-loop real-time optogenetic system Yamanouchi, Hayato M. Kamikouchi, Azusa Tanaka, Ryoya STAR Protoc Protocol In internal fertilization animals, maintaining a copulation posture facilitates the process of transporting gametes from male to female. Here, we present a protocol to investigate the neural basis for copulation posture of fruit flies using a closed-loop real-time optogenetic system. We describe steps for using deep learning analysis to enable optogenetic manipulation of neural activity only during copulation with high efficiency. This system can be applied to various animal behaviors other than copulation. For complete details on the use and execution of this protocol, please refer to Yamanouchi et al. (2023).(1) Elsevier 2023-10-02 /pmc/articles/PMC10551656/ /pubmed/37788165 http://dx.doi.org/10.1016/j.xpro.2023.102623 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Yamanouchi, Hayato M.
Kamikouchi, Azusa
Tanaka, Ryoya
Protocol to investigate the neural basis for copulation posture of Drosophila using a closed-loop real-time optogenetic system
title Protocol to investigate the neural basis for copulation posture of Drosophila using a closed-loop real-time optogenetic system
title_full Protocol to investigate the neural basis for copulation posture of Drosophila using a closed-loop real-time optogenetic system
title_fullStr Protocol to investigate the neural basis for copulation posture of Drosophila using a closed-loop real-time optogenetic system
title_full_unstemmed Protocol to investigate the neural basis for copulation posture of Drosophila using a closed-loop real-time optogenetic system
title_short Protocol to investigate the neural basis for copulation posture of Drosophila using a closed-loop real-time optogenetic system
title_sort protocol to investigate the neural basis for copulation posture of drosophila using a closed-loop real-time optogenetic system
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10551656/
https://www.ncbi.nlm.nih.gov/pubmed/37788165
http://dx.doi.org/10.1016/j.xpro.2023.102623
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