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Expression pattern, prognostic value and potential microRNA silencing of FZD8 in breast cancer

Breast cancer (BC) is one of the most widespread types of cancer affecting females, and therefore, early diagnosis is critical. BC is a complex heterogeneous disease affected by several key pathways. Among these, WNT proteins and their frizzled receptors (FZD) have been demonstrated to be crucial in...

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Autores principales: Al-Zahrani, Maryam H., Assidi, Mourad, Pushparaj, Peter Natesan, Al-Maghrabi, Jaudah, Zari, Ali, Abusanad, Atlal, Buhmeida, Abdelbaset, Abu-Elmagd, Muhammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10551865/
https://www.ncbi.nlm.nih.gov/pubmed/37809047
http://dx.doi.org/10.3892/ol.2023.14065
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author Al-Zahrani, Maryam H.
Assidi, Mourad
Pushparaj, Peter Natesan
Al-Maghrabi, Jaudah
Zari, Ali
Abusanad, Atlal
Buhmeida, Abdelbaset
Abu-Elmagd, Muhammad
author_facet Al-Zahrani, Maryam H.
Assidi, Mourad
Pushparaj, Peter Natesan
Al-Maghrabi, Jaudah
Zari, Ali
Abusanad, Atlal
Buhmeida, Abdelbaset
Abu-Elmagd, Muhammad
author_sort Al-Zahrani, Maryam H.
collection PubMed
description Breast cancer (BC) is one of the most widespread types of cancer affecting females, and therefore, early diagnosis is critical. BC is a complex heterogeneous disease affected by several key pathways. Among these, WNT proteins and their frizzled receptors (FZD) have been demonstrated to be crucial in regulating a number of cellular and molecular events in BC tumorigenesis. The role of the WNT receptor, FZD8, in BC has received minimal attention; for that reason, the present study examined the prognostic value of its protein expression pattern in a BC cohort. FZD8 cytoplasmic expression pattern analysis revealed that ~38% of the primary samples presented with a high expression profile, whereas ~63% of the samples had a low expression profile. Overall, ~46% of the malignant tissues in the lymph node-positive samples exhibited an increased FZD8 cytoplasmic expression, whereas 54% exhibited low expression levels. An increased expression of FZD8 was associated with several clinicopathological characteristics of the patients, including a low survival rate, tumor vascular invasion, tumor size and grade, and molecular subtypes. Affymetrix microarray triple-negative BC datasets were analyzed and compared with healthy breast tissues in order to predict the potential interfering microRNAs (miRNAs) in the WNT/FZD8 signaling pathway. A total of 29 miRNAs with the potential to interact with the WNT/FZD8 signaling pathway were identified, eight of which exhibited a significant prediction score. The target genes for each predicted miRNA were identified. On the whole, the findings of the present study suggest that FZD8 is a potential prognostic marker for BC, shedding some light onto the silencing mechanisms involved in the complex BC signaling.
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spelling pubmed-105518652023-10-06 Expression pattern, prognostic value and potential microRNA silencing of FZD8 in breast cancer Al-Zahrani, Maryam H. Assidi, Mourad Pushparaj, Peter Natesan Al-Maghrabi, Jaudah Zari, Ali Abusanad, Atlal Buhmeida, Abdelbaset Abu-Elmagd, Muhammad Oncol Lett Articles Breast cancer (BC) is one of the most widespread types of cancer affecting females, and therefore, early diagnosis is critical. BC is a complex heterogeneous disease affected by several key pathways. Among these, WNT proteins and their frizzled receptors (FZD) have been demonstrated to be crucial in regulating a number of cellular and molecular events in BC tumorigenesis. The role of the WNT receptor, FZD8, in BC has received minimal attention; for that reason, the present study examined the prognostic value of its protein expression pattern in a BC cohort. FZD8 cytoplasmic expression pattern analysis revealed that ~38% of the primary samples presented with a high expression profile, whereas ~63% of the samples had a low expression profile. Overall, ~46% of the malignant tissues in the lymph node-positive samples exhibited an increased FZD8 cytoplasmic expression, whereas 54% exhibited low expression levels. An increased expression of FZD8 was associated with several clinicopathological characteristics of the patients, including a low survival rate, tumor vascular invasion, tumor size and grade, and molecular subtypes. Affymetrix microarray triple-negative BC datasets were analyzed and compared with healthy breast tissues in order to predict the potential interfering microRNAs (miRNAs) in the WNT/FZD8 signaling pathway. A total of 29 miRNAs with the potential to interact with the WNT/FZD8 signaling pathway were identified, eight of which exhibited a significant prediction score. The target genes for each predicted miRNA were identified. On the whole, the findings of the present study suggest that FZD8 is a potential prognostic marker for BC, shedding some light onto the silencing mechanisms involved in the complex BC signaling. D.A. Spandidos 2023-09-21 /pmc/articles/PMC10551865/ /pubmed/37809047 http://dx.doi.org/10.3892/ol.2023.14065 Text en Copyright: © Al-Zahrani et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Al-Zahrani, Maryam H.
Assidi, Mourad
Pushparaj, Peter Natesan
Al-Maghrabi, Jaudah
Zari, Ali
Abusanad, Atlal
Buhmeida, Abdelbaset
Abu-Elmagd, Muhammad
Expression pattern, prognostic value and potential microRNA silencing of FZD8 in breast cancer
title Expression pattern, prognostic value and potential microRNA silencing of FZD8 in breast cancer
title_full Expression pattern, prognostic value and potential microRNA silencing of FZD8 in breast cancer
title_fullStr Expression pattern, prognostic value and potential microRNA silencing of FZD8 in breast cancer
title_full_unstemmed Expression pattern, prognostic value and potential microRNA silencing of FZD8 in breast cancer
title_short Expression pattern, prognostic value and potential microRNA silencing of FZD8 in breast cancer
title_sort expression pattern, prognostic value and potential microrna silencing of fzd8 in breast cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10551865/
https://www.ncbi.nlm.nih.gov/pubmed/37809047
http://dx.doi.org/10.3892/ol.2023.14065
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