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Agarwood extract mitigates alcoholic fatty liver in C57 mice via anti‑oxidation and anti‑inflammation

Alcoholic fatty liver disease (AFLD) is a disease with a high incidence rate among individuals who drink alcohol. Our previous study found that agarwood alcohol extracts (AAEs) have a protective effect against drug-induced liver damage via anti-inflammatory and antioxidant mechanisms. Therefore, we...

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Autores principales: Wang, Canhong, Gong, Bao, Peng, Deqian, Liu, Yangyang, Wu, Yulan, Wei, Jianhe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10552066/
https://www.ncbi.nlm.nih.gov/pubmed/37772395
http://dx.doi.org/10.3892/mmr.2023.13097
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author Wang, Canhong
Gong, Bao
Peng, Deqian
Liu, Yangyang
Wu, Yulan
Wei, Jianhe
author_facet Wang, Canhong
Gong, Bao
Peng, Deqian
Liu, Yangyang
Wu, Yulan
Wei, Jianhe
author_sort Wang, Canhong
collection PubMed
description Alcoholic fatty liver disease (AFLD) is a disease with a high incidence rate among individuals who drink alcohol. Our previous study found that agarwood alcohol extracts (AAEs) have a protective effect against drug-induced liver damage via anti-inflammatory and antioxidant mechanisms. Therefore, we hypothesized that agarwood may have a protective effect against AFLD. The present study assessed the potential protective effects and the underlying mechanism of action of AAEs for the treatment of an AFL in vivo model. The AFLD mouse model was established by continuous high fat diet and alcohol gavage in C57 mice. After treatment with AAEs, blood was collected, liver and adipose tissues were removed and liver and adipose indexes were analyzed. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG) and cholesterol (CHO) in serum were detected. The liver tissue was assessed using pathological sections. Biochemical methods were used to detect the levels of oxidative stress in the supernatant of liver tissue homogenate. The levels of pro-inflammatory cytokines in the serum were detected by ELISA. The protein expression levels of nuclear erythroid 2-related factor 2 (Nrf2) and nuclear factor kappa-B (NF-κB) in liver tissues were detected using western blotting. AAE treatment decreased the liver and adipose indexes, reduced the levels of AST, ALT, TG and CHO, improved the liver pathological characteristics and enhanced antioxidant and anti-inflammatory activities. In addition, AAEs increased the protein expression level of Nrf2 and decreased the protein expression level of NF-κB compared with AFL mice. AAE-treated animals exhibited reduced metabolic enzyme and blood lipid levels, demonstrated improved liver function and relieved the pathological damage of AFLD induced by consuming a high fat and alcohol diet. AAEs have potential protective effects in AFLD via antioxidant and anti-inflammatory mechanisms.
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spelling pubmed-105520662023-10-06 Agarwood extract mitigates alcoholic fatty liver in C57 mice via anti‑oxidation and anti‑inflammation Wang, Canhong Gong, Bao Peng, Deqian Liu, Yangyang Wu, Yulan Wei, Jianhe Mol Med Rep Articles Alcoholic fatty liver disease (AFLD) is a disease with a high incidence rate among individuals who drink alcohol. Our previous study found that agarwood alcohol extracts (AAEs) have a protective effect against drug-induced liver damage via anti-inflammatory and antioxidant mechanisms. Therefore, we hypothesized that agarwood may have a protective effect against AFLD. The present study assessed the potential protective effects and the underlying mechanism of action of AAEs for the treatment of an AFL in vivo model. The AFLD mouse model was established by continuous high fat diet and alcohol gavage in C57 mice. After treatment with AAEs, blood was collected, liver and adipose tissues were removed and liver and adipose indexes were analyzed. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG) and cholesterol (CHO) in serum were detected. The liver tissue was assessed using pathological sections. Biochemical methods were used to detect the levels of oxidative stress in the supernatant of liver tissue homogenate. The levels of pro-inflammatory cytokines in the serum were detected by ELISA. The protein expression levels of nuclear erythroid 2-related factor 2 (Nrf2) and nuclear factor kappa-B (NF-κB) in liver tissues were detected using western blotting. AAE treatment decreased the liver and adipose indexes, reduced the levels of AST, ALT, TG and CHO, improved the liver pathological characteristics and enhanced antioxidant and anti-inflammatory activities. In addition, AAEs increased the protein expression level of Nrf2 and decreased the protein expression level of NF-κB compared with AFL mice. AAE-treated animals exhibited reduced metabolic enzyme and blood lipid levels, demonstrated improved liver function and relieved the pathological damage of AFLD induced by consuming a high fat and alcohol diet. AAEs have potential protective effects in AFLD via antioxidant and anti-inflammatory mechanisms. D.A. Spandidos 2023-09-21 /pmc/articles/PMC10552066/ /pubmed/37772395 http://dx.doi.org/10.3892/mmr.2023.13097 Text en Copyright: © Wang et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wang, Canhong
Gong, Bao
Peng, Deqian
Liu, Yangyang
Wu, Yulan
Wei, Jianhe
Agarwood extract mitigates alcoholic fatty liver in C57 mice via anti‑oxidation and anti‑inflammation
title Agarwood extract mitigates alcoholic fatty liver in C57 mice via anti‑oxidation and anti‑inflammation
title_full Agarwood extract mitigates alcoholic fatty liver in C57 mice via anti‑oxidation and anti‑inflammation
title_fullStr Agarwood extract mitigates alcoholic fatty liver in C57 mice via anti‑oxidation and anti‑inflammation
title_full_unstemmed Agarwood extract mitigates alcoholic fatty liver in C57 mice via anti‑oxidation and anti‑inflammation
title_short Agarwood extract mitigates alcoholic fatty liver in C57 mice via anti‑oxidation and anti‑inflammation
title_sort agarwood extract mitigates alcoholic fatty liver in c57 mice via anti‑oxidation and anti‑inflammation
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10552066/
https://www.ncbi.nlm.nih.gov/pubmed/37772395
http://dx.doi.org/10.3892/mmr.2023.13097
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