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The relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression on offspring growth and body composition

BACKGROUND AND OBJECTIVE: Imprinted genes are important for the offspring development. To assess the relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression and offspring growth and body composition. METHODS: Thirty-nine overweight/obese and 25 normal weight pregnant...

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Autores principales: Argentato, Perla Pizzi, Marchesi, Jorge Augusto Petroli, Dejani, Naiara Naiana, Nakandakare, Patrícia Yury, Teles, Laísla de França da Silva, Batista, Lívia Patrícia Rodrigues, Leitão, Maria Paula Carvalho, Luzia, Liania Alves, Ramos, Ester Silveira, Rondó, Patricia Helen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10552537/
https://www.ncbi.nlm.nih.gov/pubmed/37810933
http://dx.doi.org/10.3389/fnut.2023.1170411
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author Argentato, Perla Pizzi
Marchesi, Jorge Augusto Petroli
Dejani, Naiara Naiana
Nakandakare, Patrícia Yury
Teles, Laísla de França da Silva
Batista, Lívia Patrícia Rodrigues
Leitão, Maria Paula Carvalho
Luzia, Liania Alves
Ramos, Ester Silveira
Rondó, Patricia Helen
author_facet Argentato, Perla Pizzi
Marchesi, Jorge Augusto Petroli
Dejani, Naiara Naiana
Nakandakare, Patrícia Yury
Teles, Laísla de França da Silva
Batista, Lívia Patrícia Rodrigues
Leitão, Maria Paula Carvalho
Luzia, Liania Alves
Ramos, Ester Silveira
Rondó, Patricia Helen
author_sort Argentato, Perla Pizzi
collection PubMed
description BACKGROUND AND OBJECTIVE: Imprinted genes are important for the offspring development. To assess the relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression and offspring growth and body composition. METHODS: Thirty-nine overweight/obese and 25 normal weight pregnant women were selected from the “Araraquara Cohort Study” according to their pre-pregnancy BMI. Fetal growth and body composition and newborn growth were assessed, respectively, by ultrasound and anthropometry. The methylation of H19DMR in maternal blood, cord blood, maternal decidua and placental villi tissues was evaluated by methylation-sensitive restriction endonuclease qPCR, and H19 and IGF2 expression by relative real-time PCR quantification. Multiple linear regression models explored the associations of DNA methylation and gene expression with maternal, fetal, and newborn parameters. RESULTS: H19DMR was less methylated in maternal blood of the overweight/obese group. There were associations of H19DMR methylation in cord blood with centiles of fetal biparietal diameter (BPD) and abdominal subcutaneous fat thickness and newborn head circumference (HC); H19DMR methylation in maternal decidua with fetal occipitofrontal diameter (OFD), HC, and length; H19DMR methylation in placental villi with fetal OFD, HC and abdominal subcutaneous fat thickness and with newborn HC. H19 expression in maternal decidua was associated with fetal BPD and femur length centiles and in placental villi with fetal OFD and subcutaneous arm fat. IGF2 expression in maternal decidua was associated with fetal BPD and in placental villi with fetal OFD. CONCLUSION: To our knowledge, this is the first study to demonstrate associations of imprinted genes variations at the maternal-fetal interface of the placenta and in cord blood with fetal body composition, supporting the involvement of epigenetic mechanisms in offspring growth and body composition.
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spelling pubmed-105525372023-10-06 The relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression on offspring growth and body composition Argentato, Perla Pizzi Marchesi, Jorge Augusto Petroli Dejani, Naiara Naiana Nakandakare, Patrícia Yury Teles, Laísla de França da Silva Batista, Lívia Patrícia Rodrigues Leitão, Maria Paula Carvalho Luzia, Liania Alves Ramos, Ester Silveira Rondó, Patricia Helen Front Nutr Nutrition BACKGROUND AND OBJECTIVE: Imprinted genes are important for the offspring development. To assess the relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression and offspring growth and body composition. METHODS: Thirty-nine overweight/obese and 25 normal weight pregnant women were selected from the “Araraquara Cohort Study” according to their pre-pregnancy BMI. Fetal growth and body composition and newborn growth were assessed, respectively, by ultrasound and anthropometry. The methylation of H19DMR in maternal blood, cord blood, maternal decidua and placental villi tissues was evaluated by methylation-sensitive restriction endonuclease qPCR, and H19 and IGF2 expression by relative real-time PCR quantification. Multiple linear regression models explored the associations of DNA methylation and gene expression with maternal, fetal, and newborn parameters. RESULTS: H19DMR was less methylated in maternal blood of the overweight/obese group. There were associations of H19DMR methylation in cord blood with centiles of fetal biparietal diameter (BPD) and abdominal subcutaneous fat thickness and newborn head circumference (HC); H19DMR methylation in maternal decidua with fetal occipitofrontal diameter (OFD), HC, and length; H19DMR methylation in placental villi with fetal OFD, HC and abdominal subcutaneous fat thickness and with newborn HC. H19 expression in maternal decidua was associated with fetal BPD and femur length centiles and in placental villi with fetal OFD and subcutaneous arm fat. IGF2 expression in maternal decidua was associated with fetal BPD and in placental villi with fetal OFD. CONCLUSION: To our knowledge, this is the first study to demonstrate associations of imprinted genes variations at the maternal-fetal interface of the placenta and in cord blood with fetal body composition, supporting the involvement of epigenetic mechanisms in offspring growth and body composition. Frontiers Media S.A. 2023-09-21 /pmc/articles/PMC10552537/ /pubmed/37810933 http://dx.doi.org/10.3389/fnut.2023.1170411 Text en Copyright © 2023 Argentato, Marchesi, Dejani, Nakandakare, Teles, Batista, Leitão, Luzia, Ramos and Rondó. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Argentato, Perla Pizzi
Marchesi, Jorge Augusto Petroli
Dejani, Naiara Naiana
Nakandakare, Patrícia Yury
Teles, Laísla de França da Silva
Batista, Lívia Patrícia Rodrigues
Leitão, Maria Paula Carvalho
Luzia, Liania Alves
Ramos, Ester Silveira
Rondó, Patricia Helen
The relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression on offspring growth and body composition
title The relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression on offspring growth and body composition
title_full The relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression on offspring growth and body composition
title_fullStr The relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression on offspring growth and body composition
title_full_unstemmed The relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression on offspring growth and body composition
title_short The relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression on offspring growth and body composition
title_sort relationship between obesity-related h19dmr methylation and h19 and igf2 gene expression on offspring growth and body composition
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10552537/
https://www.ncbi.nlm.nih.gov/pubmed/37810933
http://dx.doi.org/10.3389/fnut.2023.1170411
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