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Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture

The versatility of a well-known fibrous crop, Hibiscus cannabinus (kenaf) is still relatively new to many. Kenaf’s potential applications, which can be extended even into critical industries such as pharmaceutical and food industries, have always been overshadowed by its traditionally grown fiber. T...

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Autores principales: ‘Aizat Norhisham, Danial, Md Saad, Norsharina, Ahmad Usuldin, Siti Rokhiyah, Vayabari, Diwiya A G, Ilham, Zul, Ibrahim, Mohamad Faizal, Show, Pau-Loke, Wan-Mohtar, Wan Abd Al Qadr Imad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10552614/
https://www.ncbi.nlm.nih.gov/pubmed/37791464
http://dx.doi.org/10.1080/21655979.2023.2262203
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author ‘Aizat Norhisham, Danial
Md Saad, Norsharina
Ahmad Usuldin, Siti Rokhiyah
Vayabari, Diwiya A G
Ilham, Zul
Ibrahim, Mohamad Faizal
Show, Pau-Loke
Wan-Mohtar, Wan Abd Al Qadr Imad
author_facet ‘Aizat Norhisham, Danial
Md Saad, Norsharina
Ahmad Usuldin, Siti Rokhiyah
Vayabari, Diwiya A G
Ilham, Zul
Ibrahim, Mohamad Faizal
Show, Pau-Loke
Wan-Mohtar, Wan Abd Al Qadr Imad
author_sort ‘Aizat Norhisham, Danial
collection PubMed
description The versatility of a well-known fibrous crop, Hibiscus cannabinus (kenaf) is still relatively new to many. Kenaf’s potential applications, which can be extended even into critical industries such as pharmaceutical and food industries, have always been overshadowed by its traditionally grown fiber. Therefore, this study aimed to venture into the biotechnological approach in reaping the benefits of kenaf through plant cell suspension culture to maximize the production of kenaf callus biomass (KCB) and exopolysaccharide (EPS), which is deemed to be more sustainable. A growth curve was established which indicates that cultivating kenaf callus in suspension culture for 22 days gives the highest KCB (9.09 ± 1.2 g/L) and EPS (1.1 ± 0.02 g/L). Using response surface methodology (RSM), it was found that sucrose concentration, agitation speed, and naphthalene acetic acid (NAA) concentration can affect the production of KCB and EPS significantly (p < 0.05) while 2,4-dichlorophenoxy acetic acid (2,4-D) was deemed insignificant. To maximize the final yield of KCB and EPS, the final optimized variables are 50 g/L sucrose, 147.02 rpm, and 2 mg/L of NAA. To conclude, the optimized parameters for the cell suspension culture of kenaf callus serve as the blueprint for any sustainable large-scale production in the future and provide an alternative cultivating method to kenaf traditional farming.
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spelling pubmed-105526142023-10-06 Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture ‘Aizat Norhisham, Danial Md Saad, Norsharina Ahmad Usuldin, Siti Rokhiyah Vayabari, Diwiya A G Ilham, Zul Ibrahim, Mohamad Faizal Show, Pau-Loke Wan-Mohtar, Wan Abd Al Qadr Imad Bioengineered Research Article The versatility of a well-known fibrous crop, Hibiscus cannabinus (kenaf) is still relatively new to many. Kenaf’s potential applications, which can be extended even into critical industries such as pharmaceutical and food industries, have always been overshadowed by its traditionally grown fiber. Therefore, this study aimed to venture into the biotechnological approach in reaping the benefits of kenaf through plant cell suspension culture to maximize the production of kenaf callus biomass (KCB) and exopolysaccharide (EPS), which is deemed to be more sustainable. A growth curve was established which indicates that cultivating kenaf callus in suspension culture for 22 days gives the highest KCB (9.09 ± 1.2 g/L) and EPS (1.1 ± 0.02 g/L). Using response surface methodology (RSM), it was found that sucrose concentration, agitation speed, and naphthalene acetic acid (NAA) concentration can affect the production of KCB and EPS significantly (p < 0.05) while 2,4-dichlorophenoxy acetic acid (2,4-D) was deemed insignificant. To maximize the final yield of KCB and EPS, the final optimized variables are 50 g/L sucrose, 147.02 rpm, and 2 mg/L of NAA. To conclude, the optimized parameters for the cell suspension culture of kenaf callus serve as the blueprint for any sustainable large-scale production in the future and provide an alternative cultivating method to kenaf traditional farming. Taylor & Francis 2023-10-04 /pmc/articles/PMC10552614/ /pubmed/37791464 http://dx.doi.org/10.1080/21655979.2023.2262203 Text en © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.
spellingShingle Research Article
‘Aizat Norhisham, Danial
Md Saad, Norsharina
Ahmad Usuldin, Siti Rokhiyah
Vayabari, Diwiya A G
Ilham, Zul
Ibrahim, Mohamad Faizal
Show, Pau-Loke
Wan-Mohtar, Wan Abd Al Qadr Imad
Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture
title Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture
title_full Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture
title_fullStr Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture
title_full_unstemmed Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture
title_short Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture
title_sort performance of malaysian kenaf hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10552614/
https://www.ncbi.nlm.nih.gov/pubmed/37791464
http://dx.doi.org/10.1080/21655979.2023.2262203
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