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Diabetes Reshapes the Circadian Transcriptome Profile in Murine Retina

PURPOSE: Diabetic retinopathy (DR) is a common complication of diabetes and has a high prevalence. Dysregulation of circadian rhythmicity is associated with the development of DR. This research aimed to investigate rhythmical transcriptome alterations in the retina of diabetic mice. METHODS: C57BL/6...

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Autores principales: Ye, Suna, Wang, Zhijie, Ma, Jacey Hongjie, Ji, Shangli, Peng, Yanfang, Huang, Yinhua, Chen, Jiansu, Tang, Shibo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Association for Research in Vision and Ophthalmology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10552875/
https://www.ncbi.nlm.nih.gov/pubmed/37788001
http://dx.doi.org/10.1167/iovs.64.13.3
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author Ye, Suna
Wang, Zhijie
Ma, Jacey Hongjie
Ji, Shangli
Peng, Yanfang
Huang, Yinhua
Chen, Jiansu
Tang, Shibo
author_facet Ye, Suna
Wang, Zhijie
Ma, Jacey Hongjie
Ji, Shangli
Peng, Yanfang
Huang, Yinhua
Chen, Jiansu
Tang, Shibo
author_sort Ye, Suna
collection PubMed
description PURPOSE: Diabetic retinopathy (DR) is a common complication of diabetes and has a high prevalence. Dysregulation of circadian rhythmicity is associated with the development of DR. This research aimed to investigate rhythmical transcriptome alterations in the retina of diabetic mice. METHODS: C57BL/6J mice were used to establish a diabetes model by intraperitoneal injection of streptozotocin (STZ). After 12 weeks, retinas were collected continuously at 4-hour intervals over 1 day. Total RNA was extracted from normal and STZ-treated retinas and RNA sequencing was performed. Meta2d algorithm, Kyoto Encyclopedia of Genes, Phase Set Enrichment Analysis, and time-series cluster analysis were used to identify, analyze and annotate the composition, phase, and molecular functions of rhythmic transcripts in retinas. RESULTS: The retina exhibited powerful transcriptome rhythmicity. STZ-induced diabetes markedly modified the transcriptome characteristics of the circadian transcriptome in the retina, including composition, phase, and amplitude. Moreover, the diabetic mice led to re-organized temporal and clustering enrichment pathways in space and time and affected core clock machinery. CONCLUSIONS: Diabetes impairs the circadian rhythm of the transcriptomic profile of retinas. This study offers new perspectives on the negative effects of diabetes on the retina, which may provide important information for the development of new treatments for DR.
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spelling pubmed-105528752023-10-06 Diabetes Reshapes the Circadian Transcriptome Profile in Murine Retina Ye, Suna Wang, Zhijie Ma, Jacey Hongjie Ji, Shangli Peng, Yanfang Huang, Yinhua Chen, Jiansu Tang, Shibo Invest Ophthalmol Vis Sci Retina PURPOSE: Diabetic retinopathy (DR) is a common complication of diabetes and has a high prevalence. Dysregulation of circadian rhythmicity is associated with the development of DR. This research aimed to investigate rhythmical transcriptome alterations in the retina of diabetic mice. METHODS: C57BL/6J mice were used to establish a diabetes model by intraperitoneal injection of streptozotocin (STZ). After 12 weeks, retinas were collected continuously at 4-hour intervals over 1 day. Total RNA was extracted from normal and STZ-treated retinas and RNA sequencing was performed. Meta2d algorithm, Kyoto Encyclopedia of Genes, Phase Set Enrichment Analysis, and time-series cluster analysis were used to identify, analyze and annotate the composition, phase, and molecular functions of rhythmic transcripts in retinas. RESULTS: The retina exhibited powerful transcriptome rhythmicity. STZ-induced diabetes markedly modified the transcriptome characteristics of the circadian transcriptome in the retina, including composition, phase, and amplitude. Moreover, the diabetic mice led to re-organized temporal and clustering enrichment pathways in space and time and affected core clock machinery. CONCLUSIONS: Diabetes impairs the circadian rhythm of the transcriptomic profile of retinas. This study offers new perspectives on the negative effects of diabetes on the retina, which may provide important information for the development of new treatments for DR. The Association for Research in Vision and Ophthalmology 2023-10-03 /pmc/articles/PMC10552875/ /pubmed/37788001 http://dx.doi.org/10.1167/iovs.64.13.3 Text en Copyright 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
spellingShingle Retina
Ye, Suna
Wang, Zhijie
Ma, Jacey Hongjie
Ji, Shangli
Peng, Yanfang
Huang, Yinhua
Chen, Jiansu
Tang, Shibo
Diabetes Reshapes the Circadian Transcriptome Profile in Murine Retina
title Diabetes Reshapes the Circadian Transcriptome Profile in Murine Retina
title_full Diabetes Reshapes the Circadian Transcriptome Profile in Murine Retina
title_fullStr Diabetes Reshapes the Circadian Transcriptome Profile in Murine Retina
title_full_unstemmed Diabetes Reshapes the Circadian Transcriptome Profile in Murine Retina
title_short Diabetes Reshapes the Circadian Transcriptome Profile in Murine Retina
title_sort diabetes reshapes the circadian transcriptome profile in murine retina
topic Retina
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10552875/
https://www.ncbi.nlm.nih.gov/pubmed/37788001
http://dx.doi.org/10.1167/iovs.64.13.3
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