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SAT648 The Role Of Olfactory Marker Protein On Adipogenesis
Disclosure: Y. Bao: None. Y. Cho: None. E. Lee: None. Olfactory marker protein (OMP) plays a role in regulating the of olfactory transduction but is also expressed in adipose tissue. OMP serves as a regulating buffer for cyclic AMP (cAMP) levels; thus, we hypothesized that Omp may play a role in mod...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10554195/ http://dx.doi.org/10.1210/jendso/bvad114.096 |
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author | Bao, Yaru Cho, Yoon Hee Lee, Eun Jig |
author_facet | Bao, Yaru Cho, Yoon Hee Lee, Eun Jig |
author_sort | Bao, Yaru |
collection | PubMed |
description | Disclosure: Y. Bao: None. Y. Cho: None. E. Lee: None. Olfactory marker protein (OMP) plays a role in regulating the of olfactory transduction but is also expressed in adipose tissue. OMP serves as a regulating buffer for cyclic AMP (cAMP) levels; thus, we hypothesized that Omp may play a role in modulating adipocyte differentiation, a cAMP-dependent process. To determine the role of OMP in adipogenesis, we examined the differences in body weight, adipose tissue mass, and adipogenic or thermogenic gene expression of normal and Omp-knockout (KO) mice fed a high-fat diet. The cAMP production, adipogenic gene expression, and cAMP response element binding protein (CREB) phosphorylation were measured during differentiation in 3T3-L1 preadipocytes and mouse embryonic fibroblasts (MEFs). Finally, RNA sequencing was performed to find out why adipogenesis was reduced when Omp was deleted. We observed a decrease in body weight, adipose tissue mass, and the size of adipocytes in Omp-KO mice. We confirmed low cAMP production and CREB phosphorylation in adipogenesis induced in Omp(-/-) MEFs, and nuclear factor kappa B activation due to significantly reduced IκBα expression. Collectively, our results suggest that loss of OMP function inhibits adipogenesis through affecting adipocyte differentiation. Presentation: Saturday, June 17, 2023 |
format | Online Article Text |
id | pubmed-10554195 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-105541952023-10-06 SAT648 The Role Of Olfactory Marker Protein On Adipogenesis Bao, Yaru Cho, Yoon Hee Lee, Eun Jig J Endocr Soc Adipose Tissue, Appetite, & Obesity Disclosure: Y. Bao: None. Y. Cho: None. E. Lee: None. Olfactory marker protein (OMP) plays a role in regulating the of olfactory transduction but is also expressed in adipose tissue. OMP serves as a regulating buffer for cyclic AMP (cAMP) levels; thus, we hypothesized that Omp may play a role in modulating adipocyte differentiation, a cAMP-dependent process. To determine the role of OMP in adipogenesis, we examined the differences in body weight, adipose tissue mass, and adipogenic or thermogenic gene expression of normal and Omp-knockout (KO) mice fed a high-fat diet. The cAMP production, adipogenic gene expression, and cAMP response element binding protein (CREB) phosphorylation were measured during differentiation in 3T3-L1 preadipocytes and mouse embryonic fibroblasts (MEFs). Finally, RNA sequencing was performed to find out why adipogenesis was reduced when Omp was deleted. We observed a decrease in body weight, adipose tissue mass, and the size of adipocytes in Omp-KO mice. We confirmed low cAMP production and CREB phosphorylation in adipogenesis induced in Omp(-/-) MEFs, and nuclear factor kappa B activation due to significantly reduced IκBα expression. Collectively, our results suggest that loss of OMP function inhibits adipogenesis through affecting adipocyte differentiation. Presentation: Saturday, June 17, 2023 Oxford University Press 2023-10-05 /pmc/articles/PMC10554195/ http://dx.doi.org/10.1210/jendso/bvad114.096 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of the Endocrine Society. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Adipose Tissue, Appetite, & Obesity Bao, Yaru Cho, Yoon Hee Lee, Eun Jig SAT648 The Role Of Olfactory Marker Protein On Adipogenesis |
title | SAT648 The Role Of Olfactory Marker Protein On Adipogenesis |
title_full | SAT648 The Role Of Olfactory Marker Protein On Adipogenesis |
title_fullStr | SAT648 The Role Of Olfactory Marker Protein On Adipogenesis |
title_full_unstemmed | SAT648 The Role Of Olfactory Marker Protein On Adipogenesis |
title_short | SAT648 The Role Of Olfactory Marker Protein On Adipogenesis |
title_sort | sat648 the role of olfactory marker protein on adipogenesis |
topic | Adipose Tissue, Appetite, & Obesity |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10554195/ http://dx.doi.org/10.1210/jendso/bvad114.096 |
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