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SAT648 The Role Of Olfactory Marker Protein On Adipogenesis

Disclosure: Y. Bao: None. Y. Cho: None. E. Lee: None. Olfactory marker protein (OMP) plays a role in regulating the of olfactory transduction but is also expressed in adipose tissue. OMP serves as a regulating buffer for cyclic AMP (cAMP) levels; thus, we hypothesized that Omp may play a role in mod...

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Autores principales: Bao, Yaru, Cho, Yoon Hee, Lee, Eun Jig
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10554195/
http://dx.doi.org/10.1210/jendso/bvad114.096
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author Bao, Yaru
Cho, Yoon Hee
Lee, Eun Jig
author_facet Bao, Yaru
Cho, Yoon Hee
Lee, Eun Jig
author_sort Bao, Yaru
collection PubMed
description Disclosure: Y. Bao: None. Y. Cho: None. E. Lee: None. Olfactory marker protein (OMP) plays a role in regulating the of olfactory transduction but is also expressed in adipose tissue. OMP serves as a regulating buffer for cyclic AMP (cAMP) levels; thus, we hypothesized that Omp may play a role in modulating adipocyte differentiation, a cAMP-dependent process. To determine the role of OMP in adipogenesis, we examined the differences in body weight, adipose tissue mass, and adipogenic or thermogenic gene expression of normal and Omp-knockout (KO) mice fed a high-fat diet. The cAMP production, adipogenic gene expression, and cAMP response element binding protein (CREB) phosphorylation were measured during differentiation in 3T3-L1 preadipocytes and mouse embryonic fibroblasts (MEFs). Finally, RNA sequencing was performed to find out why adipogenesis was reduced when Omp was deleted. We observed a decrease in body weight, adipose tissue mass, and the size of adipocytes in Omp-KO mice. We confirmed low cAMP production and CREB phosphorylation in adipogenesis induced in Omp(-/-) MEFs, and nuclear factor kappa B activation due to significantly reduced IκBα expression. Collectively, our results suggest that loss of OMP function inhibits adipogenesis through affecting adipocyte differentiation. Presentation: Saturday, June 17, 2023
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spelling pubmed-105541952023-10-06 SAT648 The Role Of Olfactory Marker Protein On Adipogenesis Bao, Yaru Cho, Yoon Hee Lee, Eun Jig J Endocr Soc Adipose Tissue, Appetite, & Obesity Disclosure: Y. Bao: None. Y. Cho: None. E. Lee: None. Olfactory marker protein (OMP) plays a role in regulating the of olfactory transduction but is also expressed in adipose tissue. OMP serves as a regulating buffer for cyclic AMP (cAMP) levels; thus, we hypothesized that Omp may play a role in modulating adipocyte differentiation, a cAMP-dependent process. To determine the role of OMP in adipogenesis, we examined the differences in body weight, adipose tissue mass, and adipogenic or thermogenic gene expression of normal and Omp-knockout (KO) mice fed a high-fat diet. The cAMP production, adipogenic gene expression, and cAMP response element binding protein (CREB) phosphorylation were measured during differentiation in 3T3-L1 preadipocytes and mouse embryonic fibroblasts (MEFs). Finally, RNA sequencing was performed to find out why adipogenesis was reduced when Omp was deleted. We observed a decrease in body weight, adipose tissue mass, and the size of adipocytes in Omp-KO mice. We confirmed low cAMP production and CREB phosphorylation in adipogenesis induced in Omp(-/-) MEFs, and nuclear factor kappa B activation due to significantly reduced IκBα expression. Collectively, our results suggest that loss of OMP function inhibits adipogenesis through affecting adipocyte differentiation. Presentation: Saturday, June 17, 2023 Oxford University Press 2023-10-05 /pmc/articles/PMC10554195/ http://dx.doi.org/10.1210/jendso/bvad114.096 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of the Endocrine Society. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Adipose Tissue, Appetite, & Obesity
Bao, Yaru
Cho, Yoon Hee
Lee, Eun Jig
SAT648 The Role Of Olfactory Marker Protein On Adipogenesis
title SAT648 The Role Of Olfactory Marker Protein On Adipogenesis
title_full SAT648 The Role Of Olfactory Marker Protein On Adipogenesis
title_fullStr SAT648 The Role Of Olfactory Marker Protein On Adipogenesis
title_full_unstemmed SAT648 The Role Of Olfactory Marker Protein On Adipogenesis
title_short SAT648 The Role Of Olfactory Marker Protein On Adipogenesis
title_sort sat648 the role of olfactory marker protein on adipogenesis
topic Adipose Tissue, Appetite, & Obesity
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10554195/
http://dx.doi.org/10.1210/jendso/bvad114.096
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