FRI030 The Clinical Significance Of The Oxidative Status Evaluation Among Insulin Resistant Overweight And Obese Patients

Disclosure: A. Popławska-Kita: None. A. Buczyńska: None. E. Duraj: None. I. Sidorkiewicz: None. K. Łuka: None. K. Siewko: None. A. Adamska: None. A. Krętowski: None. Insulin resistance is a prevalent syndrome, developing and occurring simultaneously with weight gain, considering as predisposing factor for type 2 diabetes mellitus (T2DM), frequently resulting from metabolic syndrome development. Despite significant progress in the treatment of overweight and obesity, these patients are still at risk of developing chronic complications related to insulin resistance, such as T2DM and cardiovascular diseases, which are a significant clinical problem. In the pathogenesis of complications of insulin resistance among obese and overweight patients, an increasing role is attributed to oxidative stress. In physiological conditions reactive oxygen species (ROS) are formed from molecular oxygen in the cellular respiration process, but in concentrations exceeding the physiological are leading to the oxidative damage of macromolecules, such as: proteins, lipids, DNA. Furthermore, ROS may cause insulin resistance in the peripheral tissues by affecting various points in insulin receptor signal transduction increasing the risk of continued further weight gain. In this study, oxidative status among insulin resistant obese and overweight patients was determined. For the purpose of this study, 50 patients with obesity and insulin resistance (BMI>30), 50 overweight patients with insulin resistance (BMI>25) and 50 healthy volunteers (BMI<25) were enrolled. The oxidative status was determined using total oxidative capacity (TOC) and total antioxidant capacity (TAC). The body composition was assessed using the dual energy x-ray absorptiometry method (DXA). Insulin resistance was evaluated using the homeostatic model assessment for insulin resistance (HOMA) calculation. All patients underwent oral glucose tolerance test (OGTT) and glucose and insulin measurements at times: 0, 60, 120, 180 and 240 minutes. Overweight and obese patients presented increased TOC concentration and decreased TAC concentration comparing to control group (all, p<0.05). There were no differences in oxidative status between the obese and overweight groups (p>0.05). In the overweight study group, TOC moderately correlated with body weight, TAC moderately correlated with body fat mass and glucose concentration at 240 minutes in the OGTT. In the obese group, TOC moderately correlated with BMI, body fat mass and glucose levels in 240 minutes of the OGTT, and TAC correlated with body weight and fasting glucose.The results underline the importance of determining the oxidative status in obese and overweight patients, where oxidative status is related to glucose metabolism and body mass composition. Nevertheless, more research is needed to elucidate the effect of increased oxidative stress on the insulin resistance development. Presentation: Friday, June 16, 2023