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SAT652 The Expression Of The Immunoproteasome Subunit Psmb9 Is Related To Obesity And The Differentiation Of Human Preadipocytes

Disclosure: Y. Cui: None. X. Zhang: None. L. You: None. H. Zhong: None. X. Cui: None. C. Ji: None. Obesity and its associated chronic diseases, such as type 2 diabetes mellitus, hypertension, and coronary artery disease, afflicting adults and children globally. PSMB9, a proteolytic subunit of the im...

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Autores principales: Cui, Yan, Zhang, Xiaoxiao, You, Lianghui, Zhong, Hong, Cui, Xianwei, Ji, Chenbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10554940/
http://dx.doi.org/10.1210/jendso/bvad114.100
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author Cui, Yan
Zhang, Xiaoxiao
You, Lianghui
Zhong, Hong
Cui, Xianwei
Ji, Chenbo
author_facet Cui, Yan
Zhang, Xiaoxiao
You, Lianghui
Zhong, Hong
Cui, Xianwei
Ji, Chenbo
author_sort Cui, Yan
collection PubMed
description Disclosure: Y. Cui: None. X. Zhang: None. L. You: None. H. Zhong: None. X. Cui: None. C. Ji: None. Obesity and its associated chronic diseases, such as type 2 diabetes mellitus, hypertension, and coronary artery disease, afflicting adults and children globally. PSMB9, a proteolytic subunit of the immunoproteasome that shapes the repertoire of antigenic peptides, is hypothesized to be associated with obesity. To investigate this association, we examined PSMB9 expression in primary mature adipocytes from obese and normal individuals. LC-MS/MS-PRM was used to quantify PSMB9 content in primary mature adipocytes, revealing a negative correlation between PSMB9 content and obesity while positively correlated with high-density lipoprotein levels. Furthermore, we examined the effect of PSMB9 on human preadipocyte differentiation. During the early stages of differentiation, the expression of PSMB9 was at its highest level, decreasing as preadipocytes matured. Overexpression of PSMB9 significantly inhibited preadipocyte differentiation, resulting in reduced lipid droplets and decreased expression levels of classical differentiation markers such as FABP4, C/EBPα, and PPARγ. These findings suggest that PSMB9 could be a potential therapeutic target for preventing and treating obesity and metabolic disorders. In conclusion, our study provides insights into the role of the immunoproteasome in obesity and highlights the importance of further exploration of this pathway. Presentation: Saturday, June 17, 2023
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spelling pubmed-105549402023-10-06 SAT652 The Expression Of The Immunoproteasome Subunit Psmb9 Is Related To Obesity And The Differentiation Of Human Preadipocytes Cui, Yan Zhang, Xiaoxiao You, Lianghui Zhong, Hong Cui, Xianwei Ji, Chenbo J Endocr Soc Adipose Tissue, Appetite, & Obesity Disclosure: Y. Cui: None. X. Zhang: None. L. You: None. H. Zhong: None. X. Cui: None. C. Ji: None. Obesity and its associated chronic diseases, such as type 2 diabetes mellitus, hypertension, and coronary artery disease, afflicting adults and children globally. PSMB9, a proteolytic subunit of the immunoproteasome that shapes the repertoire of antigenic peptides, is hypothesized to be associated with obesity. To investigate this association, we examined PSMB9 expression in primary mature adipocytes from obese and normal individuals. LC-MS/MS-PRM was used to quantify PSMB9 content in primary mature adipocytes, revealing a negative correlation between PSMB9 content and obesity while positively correlated with high-density lipoprotein levels. Furthermore, we examined the effect of PSMB9 on human preadipocyte differentiation. During the early stages of differentiation, the expression of PSMB9 was at its highest level, decreasing as preadipocytes matured. Overexpression of PSMB9 significantly inhibited preadipocyte differentiation, resulting in reduced lipid droplets and decreased expression levels of classical differentiation markers such as FABP4, C/EBPα, and PPARγ. These findings suggest that PSMB9 could be a potential therapeutic target for preventing and treating obesity and metabolic disorders. In conclusion, our study provides insights into the role of the immunoproteasome in obesity and highlights the importance of further exploration of this pathway. Presentation: Saturday, June 17, 2023 Oxford University Press 2023-10-05 /pmc/articles/PMC10554940/ http://dx.doi.org/10.1210/jendso/bvad114.100 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of the Endocrine Society. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Adipose Tissue, Appetite, & Obesity
Cui, Yan
Zhang, Xiaoxiao
You, Lianghui
Zhong, Hong
Cui, Xianwei
Ji, Chenbo
SAT652 The Expression Of The Immunoproteasome Subunit Psmb9 Is Related To Obesity And The Differentiation Of Human Preadipocytes
title SAT652 The Expression Of The Immunoproteasome Subunit Psmb9 Is Related To Obesity And The Differentiation Of Human Preadipocytes
title_full SAT652 The Expression Of The Immunoproteasome Subunit Psmb9 Is Related To Obesity And The Differentiation Of Human Preadipocytes
title_fullStr SAT652 The Expression Of The Immunoproteasome Subunit Psmb9 Is Related To Obesity And The Differentiation Of Human Preadipocytes
title_full_unstemmed SAT652 The Expression Of The Immunoproteasome Subunit Psmb9 Is Related To Obesity And The Differentiation Of Human Preadipocytes
title_short SAT652 The Expression Of The Immunoproteasome Subunit Psmb9 Is Related To Obesity And The Differentiation Of Human Preadipocytes
title_sort sat652 the expression of the immunoproteasome subunit psmb9 is related to obesity and the differentiation of human preadipocytes
topic Adipose Tissue, Appetite, & Obesity
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10554940/
http://dx.doi.org/10.1210/jendso/bvad114.100
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