THU148 Changes In Cortisol:Cortisone Ratio In Youth With Obesity One Year Following Sleeve Gastrectomy Compared To Non-surgical Controls.

Disclosure: A.P. López López: None. S. Tuli: None. I. Becetti: None. M. Lauze: None. C.C. Pedreira: None. M. Bredella: None. M. Misra: None. V. Singhal: None. Introduction: Glucocorticoid metabolites are associated with body composition measures and are altered with weight status. Metabolic and bariatric surgery (MBS) results in significant changes in weight and body composition. However, effects of MBS on glucocorticoid metabolites are unknown. Objective: To evaluate (i) changes in the cortisol:cortisone ratio [indicator of 11β-Hydroxysteroid Dehydrogenase (11 β -HSD) global activity] in youth with obesity 12 months after sleeve gastrectomy (SG) compared with non-surgical controls with obesity (NS), and (ii) associations of these changes with body composition changes. Methods: 60 participants 13-25 years old with obesity (44 female) were followed for 12 months. 28 participants underwent SG and 32 were followed with routine care (non-surgical, NS). Fasting blood was assessed for cortisol and cortisone using LC-MS as part of metabolomic analysis, and the cortisol:cortisone ratio calculated. Single slice MRI of the abdomen was performed to assess subcutaneous, visceral and total adipose tissue (SAT, VAT and TAT) and DXA for percent (%) body fat. Results: At baseline, groups did not differ for age or sex. SG vs. NS had higher median BMI [46.4 (41.0, 52.8) vs. 41.9 (37.9, 46.9) kg/m(2), p=0.007] and % body fat [51.2 (47.8, 52.3) vs. 47.2 (39.0, 47.2) %, p=0.005)]. Groups did not differ for baseline VAT, SAT, TAT and cortisol:cortisone [10.47 (9.52, 13.86) vs. 11.05 (6.37, 14.79), p=0.58]. Significant reductions were noted over 12 months in BMI, BMIz, % body fat, TAT, VAT, SAT and VAT/SAT within the SG vs. NS groups (p ≤ 0.03). No within group changes in cortisol:cortisone over one year were noted in the NS group, but the ratio trended to decrease in the SG group [-1.40 (-5.08, 0.06), p=0.08]. No associations were found between changes in cortisol:cortisone and changes in body composition. Conclusions: The cortisol:cortisone ratio tended to decrease 12 months following SG. No associations were found between changes in the cortisol:cortisone ratio and body composition changes. Further studies with larger numbers of participants are necessary to confirm these findings. Presentation: Thursday, June 15, 2023