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OR34-05 Second Phase Insulin Secretion Varies By Time Of Day And Body Mass Index
Disclosure: S. Naaman: None. S. Sintetas: None. E. Van Cauter: None. E.C. Hanlon: None. Diurnal variation in insulin secretion (ISR) is documented in healthy adults with and without obesity. However, in previous studies, the caloric content of meals varied across the day or the analysis focused sole...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10555659/ http://dx.doi.org/10.1210/jendso/bvad114.1044 |
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author | Naaman, Sandra Sintetas, Stephanie Van Cauter, Eve Hanlon, Erin C |
author_facet | Naaman, Sandra Sintetas, Stephanie Van Cauter, Eve Hanlon, Erin C |
author_sort | Naaman, Sandra |
collection | PubMed |
description | Disclosure: S. Naaman: None. S. Sintetas: None. E. Van Cauter: None. E.C. Hanlon: None. Diurnal variation in insulin secretion (ISR) is documented in healthy adults with and without obesity. However, in previous studies, the caloric content of meals varied across the day or the analysis focused solely on morning versus evening differences. It remains unclear if dietary intake timing is associated with metabolic response differences to the same dietary challenge if presented across a full day in individuals categorized as lean (L), with overweight (OW) or obesity (OB). Thus, we examined ISR following consumption of identical meals, equally spaced across the day, in a lab setting with controlled sleep and energy expenditure. Our study included 74 individuals; 41 lean (19 women), 14 overweight (4 women), and 19 obese (14 women). Obstructive sleep apnea (OSA), a known confounder of glucose metabolism, was an exclusion criterion. Mean age between groups was similar (L, 25.2 ± 5.3yrs vs OW, 27.0 ± 6.6yrs vs OB, 32.2 ± 7.6yrs). BMI was 38 ± 5.9 kg/m(2) in the OB, 27 ± 1.2 kg/m(2) in the OW, vs 22 ± 1.8 kg/m(2) in the L groups, respectively (p < 0.0001). We collected blood samples continuously at regular intervals over a 24hr period in which participants ate isocaloric identical high carbohydrate meals (20% fat, 68% carbohydrate, 12% protein) at 0900, 1400, and 1900; each meal was 33% of total caloric intake. Serum C-peptide was measured using high-sensitivity chemiluminescence assays (Immulite). ISR was derived from plasma C-peptide levels by mathematical deconvolution using a two-compartment model for C-peptide clearance. Each profile was expressed as a percentage of individual 24hr mean ISR to examine the profile wave shape independently of individual differences in mean ISR. We examined 2 phases of ISR following meal ingestion; 1(st) phase (AUC, 0 - 1.5hrs) and 2(nd) phase (AUC, 1.5hrs - 3.5hrs). The profiles were analyzed with ANOVA for repeated measures with participants as a random factor and mealtime, group (L, OW, OB), and interaction “mealtime by group” as fixed effects. Despite 3 equally spaced identical meals, significant differences emerged. Irrespective of BMI, 1(st) phase relative ISR was similar across breakfast, lunch and dinner. Second phase relative ISR, however, revealed significant time of day effects; with midday eliciting the highest relative ISR for all groups (p = 0.0002). Significant time x group effects emerged in the 2(nd) phase such that relative ISR varied across the day, differently for each group (p < 0.0001). Specifically, 2(nd) phase relative ISR was lowest following dinner in those with obesity. This is the first study to compare 24hr profiles of ISR in healthy individuals at multiple BMI ranges, without OSA, in an experimental setting of 3 equally spaced identical meals. These findings imply that the midday meal elicits the highest ISRs, irrespective of BMI. Further, BMI impacts ISR across the day in response to identical meals. These findings have implications for obesity management. Presentation: Sunday, June 18, 2023 |
format | Online Article Text |
id | pubmed-10555659 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-105556592023-10-07 OR34-05 Second Phase Insulin Secretion Varies By Time Of Day And Body Mass Index Naaman, Sandra Sintetas, Stephanie Van Cauter, Eve Hanlon, Erin C J Endocr Soc Diabetes And Glucose Metabolism Disclosure: S. Naaman: None. S. Sintetas: None. E. Van Cauter: None. E.C. Hanlon: None. Diurnal variation in insulin secretion (ISR) is documented in healthy adults with and without obesity. However, in previous studies, the caloric content of meals varied across the day or the analysis focused solely on morning versus evening differences. It remains unclear if dietary intake timing is associated with metabolic response differences to the same dietary challenge if presented across a full day in individuals categorized as lean (L), with overweight (OW) or obesity (OB). Thus, we examined ISR following consumption of identical meals, equally spaced across the day, in a lab setting with controlled sleep and energy expenditure. Our study included 74 individuals; 41 lean (19 women), 14 overweight (4 women), and 19 obese (14 women). Obstructive sleep apnea (OSA), a known confounder of glucose metabolism, was an exclusion criterion. Mean age between groups was similar (L, 25.2 ± 5.3yrs vs OW, 27.0 ± 6.6yrs vs OB, 32.2 ± 7.6yrs). BMI was 38 ± 5.9 kg/m(2) in the OB, 27 ± 1.2 kg/m(2) in the OW, vs 22 ± 1.8 kg/m(2) in the L groups, respectively (p < 0.0001). We collected blood samples continuously at regular intervals over a 24hr period in which participants ate isocaloric identical high carbohydrate meals (20% fat, 68% carbohydrate, 12% protein) at 0900, 1400, and 1900; each meal was 33% of total caloric intake. Serum C-peptide was measured using high-sensitivity chemiluminescence assays (Immulite). ISR was derived from plasma C-peptide levels by mathematical deconvolution using a two-compartment model for C-peptide clearance. Each profile was expressed as a percentage of individual 24hr mean ISR to examine the profile wave shape independently of individual differences in mean ISR. We examined 2 phases of ISR following meal ingestion; 1(st) phase (AUC, 0 - 1.5hrs) and 2(nd) phase (AUC, 1.5hrs - 3.5hrs). The profiles were analyzed with ANOVA for repeated measures with participants as a random factor and mealtime, group (L, OW, OB), and interaction “mealtime by group” as fixed effects. Despite 3 equally spaced identical meals, significant differences emerged. Irrespective of BMI, 1(st) phase relative ISR was similar across breakfast, lunch and dinner. Second phase relative ISR, however, revealed significant time of day effects; with midday eliciting the highest relative ISR for all groups (p = 0.0002). Significant time x group effects emerged in the 2(nd) phase such that relative ISR varied across the day, differently for each group (p < 0.0001). Specifically, 2(nd) phase relative ISR was lowest following dinner in those with obesity. This is the first study to compare 24hr profiles of ISR in healthy individuals at multiple BMI ranges, without OSA, in an experimental setting of 3 equally spaced identical meals. These findings imply that the midday meal elicits the highest ISRs, irrespective of BMI. Further, BMI impacts ISR across the day in response to identical meals. These findings have implications for obesity management. Presentation: Sunday, June 18, 2023 Oxford University Press 2023-10-05 /pmc/articles/PMC10555659/ http://dx.doi.org/10.1210/jendso/bvad114.1044 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of the Endocrine Society. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Diabetes And Glucose Metabolism Naaman, Sandra Sintetas, Stephanie Van Cauter, Eve Hanlon, Erin C OR34-05 Second Phase Insulin Secretion Varies By Time Of Day And Body Mass Index |
title | OR34-05 Second Phase Insulin Secretion Varies By Time Of Day And Body Mass Index |
title_full | OR34-05 Second Phase Insulin Secretion Varies By Time Of Day And Body Mass Index |
title_fullStr | OR34-05 Second Phase Insulin Secretion Varies By Time Of Day And Body Mass Index |
title_full_unstemmed | OR34-05 Second Phase Insulin Secretion Varies By Time Of Day And Body Mass Index |
title_short | OR34-05 Second Phase Insulin Secretion Varies By Time Of Day And Body Mass Index |
title_sort | or34-05 second phase insulin secretion varies by time of day and body mass index |
topic | Diabetes And Glucose Metabolism |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10555659/ http://dx.doi.org/10.1210/jendso/bvad114.1044 |
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