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Lung mechanics showing sex‐based differences and circadian time‐of‐day response to bleomycin‐induced lung injury in mice
Idiopathic pulmonary fibrosis (IPF) is a progressive disease that impairs lung mechanical properties due to dysregulated extracellular matrix remodeling. Lung function assessment is an important physiological endpoint in the mouse model of pulmonary fibrosis (PF) that has gained a broader scientific...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10555704/ https://www.ncbi.nlm.nih.gov/pubmed/37798115 http://dx.doi.org/10.14814/phy2.15828 |
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author | Prasad, Chandrashekhar Duraisamy, Santhosh Kumar Sundar, Isaac Kirubakaran |
author_facet | Prasad, Chandrashekhar Duraisamy, Santhosh Kumar Sundar, Isaac Kirubakaran |
author_sort | Prasad, Chandrashekhar |
collection | PubMed |
description | Idiopathic pulmonary fibrosis (IPF) is a progressive disease that impairs lung mechanical properties due to dysregulated extracellular matrix remodeling. Lung function assessment is an important physiological endpoint in the mouse model of pulmonary fibrosis (PF) that has gained a broader scientific acceptance in the field. IPF pathophysiology shows sex‐based differences, disproportionately affecting more men compared to women. Prior reports suggest that the circadian clock is perturbed during the pathogenesis of PF. We have comprehensively assessed the sex‐based differences and time‐of‐day response (at Zeitgeber time: ZT0/6:00 a.m. or ZT12/6 p.m.) in lung mechanics among sham (control) versus bleomycin (BLM)‐challenged female and male (C57BL/6: WT) mice using Flexi‐vent. BLM challenge altered lung function significantly in males in both total lung (reduced dynamic compliance, and increased resistance and elastance) as well as lung tissue‐specific parameters (increased tissue elastance and tissue damping) but less pronounced in females. BLM‐challenged mice showed a time‐of‐day response in lung function at ZT0 versus ZT12, which was pronounced in the ZT0 BLM group. Overall, these findings provide a comprehensive analysis of altered lung function in female and male mice and the time‐of‐day difference in lung function parameters following BLM‐induced lung fibrosis. |
format | Online Article Text |
id | pubmed-10555704 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-105557042023-10-07 Lung mechanics showing sex‐based differences and circadian time‐of‐day response to bleomycin‐induced lung injury in mice Prasad, Chandrashekhar Duraisamy, Santhosh Kumar Sundar, Isaac Kirubakaran Physiol Rep Original Articles Idiopathic pulmonary fibrosis (IPF) is a progressive disease that impairs lung mechanical properties due to dysregulated extracellular matrix remodeling. Lung function assessment is an important physiological endpoint in the mouse model of pulmonary fibrosis (PF) that has gained a broader scientific acceptance in the field. IPF pathophysiology shows sex‐based differences, disproportionately affecting more men compared to women. Prior reports suggest that the circadian clock is perturbed during the pathogenesis of PF. We have comprehensively assessed the sex‐based differences and time‐of‐day response (at Zeitgeber time: ZT0/6:00 a.m. or ZT12/6 p.m.) in lung mechanics among sham (control) versus bleomycin (BLM)‐challenged female and male (C57BL/6: WT) mice using Flexi‐vent. BLM challenge altered lung function significantly in males in both total lung (reduced dynamic compliance, and increased resistance and elastance) as well as lung tissue‐specific parameters (increased tissue elastance and tissue damping) but less pronounced in females. BLM‐challenged mice showed a time‐of‐day response in lung function at ZT0 versus ZT12, which was pronounced in the ZT0 BLM group. Overall, these findings provide a comprehensive analysis of altered lung function in female and male mice and the time‐of‐day difference in lung function parameters following BLM‐induced lung fibrosis. John Wiley and Sons Inc. 2023-10-05 /pmc/articles/PMC10555704/ /pubmed/37798115 http://dx.doi.org/10.14814/phy2.15828 Text en © 2023 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Prasad, Chandrashekhar Duraisamy, Santhosh Kumar Sundar, Isaac Kirubakaran Lung mechanics showing sex‐based differences and circadian time‐of‐day response to bleomycin‐induced lung injury in mice |
title | Lung mechanics showing sex‐based differences and circadian time‐of‐day response to bleomycin‐induced lung injury in mice |
title_full | Lung mechanics showing sex‐based differences and circadian time‐of‐day response to bleomycin‐induced lung injury in mice |
title_fullStr | Lung mechanics showing sex‐based differences and circadian time‐of‐day response to bleomycin‐induced lung injury in mice |
title_full_unstemmed | Lung mechanics showing sex‐based differences and circadian time‐of‐day response to bleomycin‐induced lung injury in mice |
title_short | Lung mechanics showing sex‐based differences and circadian time‐of‐day response to bleomycin‐induced lung injury in mice |
title_sort | lung mechanics showing sex‐based differences and circadian time‐of‐day response to bleomycin‐induced lung injury in mice |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10555704/ https://www.ncbi.nlm.nih.gov/pubmed/37798115 http://dx.doi.org/10.14814/phy2.15828 |
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