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A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea

Male sterility has been used for crop hybrid breeding for a long time. It has contributed greatly to crop yield increase. However, the genetic basis of male sterility has not been fully elucidated. Here, we report map-based cloning of the cabbage (Brassica oleracea) dominant male-sterile gene Ms-cd1...

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Autores principales: Han, Fengqing, Yuan, Kaiwen, Sun, Wenru, Zhang, Xiaoli, Liu, Xing, Zhao, Xinyu, Yang, Limei, Wang, Yong, Ji, Jialei, Liu, Yumei, Li, Zhansheng, Zhang, Jinzhe, Zhang, Chunzhi, Huang, Sanwen, Zhang, Yangyong, Fang, Zhiyuan, Lv, Honghao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556095/
https://www.ncbi.nlm.nih.gov/pubmed/37798291
http://dx.doi.org/10.1038/s41467-023-41916-0
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author Han, Fengqing
Yuan, Kaiwen
Sun, Wenru
Zhang, Xiaoli
Liu, Xing
Zhao, Xinyu
Yang, Limei
Wang, Yong
Ji, Jialei
Liu, Yumei
Li, Zhansheng
Zhang, Jinzhe
Zhang, Chunzhi
Huang, Sanwen
Zhang, Yangyong
Fang, Zhiyuan
Lv, Honghao
author_facet Han, Fengqing
Yuan, Kaiwen
Sun, Wenru
Zhang, Xiaoli
Liu, Xing
Zhao, Xinyu
Yang, Limei
Wang, Yong
Ji, Jialei
Liu, Yumei
Li, Zhansheng
Zhang, Jinzhe
Zhang, Chunzhi
Huang, Sanwen
Zhang, Yangyong
Fang, Zhiyuan
Lv, Honghao
author_sort Han, Fengqing
collection PubMed
description Male sterility has been used for crop hybrid breeding for a long time. It has contributed greatly to crop yield increase. However, the genetic basis of male sterility has not been fully elucidated. Here, we report map-based cloning of the cabbage (Brassica oleracea) dominant male-sterile gene Ms-cd1 and reveal that it encodes a PHD-finger motif transcription factor. A natural allele Ms-cd1(PΔ−597,) resulting from a 1-bp deletion in the promoter, confers dominant genic male sterility (DGMS), whereas loss-of-function ms-cd1 mutant shows recessive male sterility. We also show that the ethylene response factor BoERF1L represses the expression of Ms-cd1 by directly binding to its promoter; however, the 1-bp deletion in Ms-cd1(PΔ−597) affects the binding. Furthermore, ectopic expression of Ms-cd1(PΔ−597) confers DGMS in both dicotyledonous and monocotyledonous plant species. We thus propose that the DGMS system could be useful for breeding hybrids of multiple crop species.
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spelling pubmed-105560952023-10-07 A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea Han, Fengqing Yuan, Kaiwen Sun, Wenru Zhang, Xiaoli Liu, Xing Zhao, Xinyu Yang, Limei Wang, Yong Ji, Jialei Liu, Yumei Li, Zhansheng Zhang, Jinzhe Zhang, Chunzhi Huang, Sanwen Zhang, Yangyong Fang, Zhiyuan Lv, Honghao Nat Commun Article Male sterility has been used for crop hybrid breeding for a long time. It has contributed greatly to crop yield increase. However, the genetic basis of male sterility has not been fully elucidated. Here, we report map-based cloning of the cabbage (Brassica oleracea) dominant male-sterile gene Ms-cd1 and reveal that it encodes a PHD-finger motif transcription factor. A natural allele Ms-cd1(PΔ−597,) resulting from a 1-bp deletion in the promoter, confers dominant genic male sterility (DGMS), whereas loss-of-function ms-cd1 mutant shows recessive male sterility. We also show that the ethylene response factor BoERF1L represses the expression of Ms-cd1 by directly binding to its promoter; however, the 1-bp deletion in Ms-cd1(PΔ−597) affects the binding. Furthermore, ectopic expression of Ms-cd1(PΔ−597) confers DGMS in both dicotyledonous and monocotyledonous plant species. We thus propose that the DGMS system could be useful for breeding hybrids of multiple crop species. Nature Publishing Group UK 2023-10-05 /pmc/articles/PMC10556095/ /pubmed/37798291 http://dx.doi.org/10.1038/s41467-023-41916-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Han, Fengqing
Yuan, Kaiwen
Sun, Wenru
Zhang, Xiaoli
Liu, Xing
Zhao, Xinyu
Yang, Limei
Wang, Yong
Ji, Jialei
Liu, Yumei
Li, Zhansheng
Zhang, Jinzhe
Zhang, Chunzhi
Huang, Sanwen
Zhang, Yangyong
Fang, Zhiyuan
Lv, Honghao
A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea
title A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea
title_full A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea
title_fullStr A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea
title_full_unstemmed A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea
title_short A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea
title_sort natural mutation in the promoter of ms-cd1 causes dominant male sterility in brassica oleracea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556095/
https://www.ncbi.nlm.nih.gov/pubmed/37798291
http://dx.doi.org/10.1038/s41467-023-41916-0
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