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A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea
Male sterility has been used for crop hybrid breeding for a long time. It has contributed greatly to crop yield increase. However, the genetic basis of male sterility has not been fully elucidated. Here, we report map-based cloning of the cabbage (Brassica oleracea) dominant male-sterile gene Ms-cd1...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556095/ https://www.ncbi.nlm.nih.gov/pubmed/37798291 http://dx.doi.org/10.1038/s41467-023-41916-0 |
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author | Han, Fengqing Yuan, Kaiwen Sun, Wenru Zhang, Xiaoli Liu, Xing Zhao, Xinyu Yang, Limei Wang, Yong Ji, Jialei Liu, Yumei Li, Zhansheng Zhang, Jinzhe Zhang, Chunzhi Huang, Sanwen Zhang, Yangyong Fang, Zhiyuan Lv, Honghao |
author_facet | Han, Fengqing Yuan, Kaiwen Sun, Wenru Zhang, Xiaoli Liu, Xing Zhao, Xinyu Yang, Limei Wang, Yong Ji, Jialei Liu, Yumei Li, Zhansheng Zhang, Jinzhe Zhang, Chunzhi Huang, Sanwen Zhang, Yangyong Fang, Zhiyuan Lv, Honghao |
author_sort | Han, Fengqing |
collection | PubMed |
description | Male sterility has been used for crop hybrid breeding for a long time. It has contributed greatly to crop yield increase. However, the genetic basis of male sterility has not been fully elucidated. Here, we report map-based cloning of the cabbage (Brassica oleracea) dominant male-sterile gene Ms-cd1 and reveal that it encodes a PHD-finger motif transcription factor. A natural allele Ms-cd1(PΔ−597,) resulting from a 1-bp deletion in the promoter, confers dominant genic male sterility (DGMS), whereas loss-of-function ms-cd1 mutant shows recessive male sterility. We also show that the ethylene response factor BoERF1L represses the expression of Ms-cd1 by directly binding to its promoter; however, the 1-bp deletion in Ms-cd1(PΔ−597) affects the binding. Furthermore, ectopic expression of Ms-cd1(PΔ−597) confers DGMS in both dicotyledonous and monocotyledonous plant species. We thus propose that the DGMS system could be useful for breeding hybrids of multiple crop species. |
format | Online Article Text |
id | pubmed-10556095 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-105560952023-10-07 A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea Han, Fengqing Yuan, Kaiwen Sun, Wenru Zhang, Xiaoli Liu, Xing Zhao, Xinyu Yang, Limei Wang, Yong Ji, Jialei Liu, Yumei Li, Zhansheng Zhang, Jinzhe Zhang, Chunzhi Huang, Sanwen Zhang, Yangyong Fang, Zhiyuan Lv, Honghao Nat Commun Article Male sterility has been used for crop hybrid breeding for a long time. It has contributed greatly to crop yield increase. However, the genetic basis of male sterility has not been fully elucidated. Here, we report map-based cloning of the cabbage (Brassica oleracea) dominant male-sterile gene Ms-cd1 and reveal that it encodes a PHD-finger motif transcription factor. A natural allele Ms-cd1(PΔ−597,) resulting from a 1-bp deletion in the promoter, confers dominant genic male sterility (DGMS), whereas loss-of-function ms-cd1 mutant shows recessive male sterility. We also show that the ethylene response factor BoERF1L represses the expression of Ms-cd1 by directly binding to its promoter; however, the 1-bp deletion in Ms-cd1(PΔ−597) affects the binding. Furthermore, ectopic expression of Ms-cd1(PΔ−597) confers DGMS in both dicotyledonous and monocotyledonous plant species. We thus propose that the DGMS system could be useful for breeding hybrids of multiple crop species. Nature Publishing Group UK 2023-10-05 /pmc/articles/PMC10556095/ /pubmed/37798291 http://dx.doi.org/10.1038/s41467-023-41916-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Han, Fengqing Yuan, Kaiwen Sun, Wenru Zhang, Xiaoli Liu, Xing Zhao, Xinyu Yang, Limei Wang, Yong Ji, Jialei Liu, Yumei Li, Zhansheng Zhang, Jinzhe Zhang, Chunzhi Huang, Sanwen Zhang, Yangyong Fang, Zhiyuan Lv, Honghao A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea |
title | A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea |
title_full | A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea |
title_fullStr | A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea |
title_full_unstemmed | A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea |
title_short | A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea |
title_sort | natural mutation in the promoter of ms-cd1 causes dominant male sterility in brassica oleracea |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556095/ https://www.ncbi.nlm.nih.gov/pubmed/37798291 http://dx.doi.org/10.1038/s41467-023-41916-0 |
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