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First report of a novel polymorphism and genetic characteristics of the leporine prion protein (PRNP) gene

Transmissible spongiform encephalopathies (TSEs) have been reported in a broad spectrum of hosts. The genetic polymorphisms and characteristics of the prion protein (PRNP) gene have a vital impact on the development of TSEs. Notably, natural TSE infection cases have never been reported in rabbits, a...

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Autores principales: Kim, Dong-Ju, Kim, Yong-Chan, Jeong, Byung-Hoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556520/
https://www.ncbi.nlm.nih.gov/pubmed/37808111
http://dx.doi.org/10.3389/fvets.2023.1229369
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author Kim, Dong-Ju
Kim, Yong-Chan
Jeong, Byung-Hoon
author_facet Kim, Dong-Ju
Kim, Yong-Chan
Jeong, Byung-Hoon
author_sort Kim, Dong-Ju
collection PubMed
description Transmissible spongiform encephalopathies (TSEs) have been reported in a broad spectrum of hosts. The genetic polymorphisms and characteristics of the prion protein (PRNP) gene have a vital impact on the development of TSEs. Notably, natural TSE infection cases have never been reported in rabbits, and genetic variations of the leporine PRNP gene have not been investigated to date. To identify leporine PRNP gene polymorphism, we performed amplicon sequencing in 203 rabbits. We report a novel single nucleotide polymorphism on the leporine PRNP gene. In addition, we performed a comparative analysis of amino acid sequences of prion protein (PrP) across several hosts using ClustalW2. Furthermore, we evaluated the effect of changes of unique leporine PrP amino acids with those conserved among various species using Swiss-Pdb Viewer. Interestingly, we found seven unique leporine amino acids, and the change of unique leporine amino acids with those conserved among other species, including S175N, Q221K, Q221R, A226Y, A230G, and A230S, was predicted to reduce hydrogen bonds in leporine PrP.
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spelling pubmed-105565202023-10-07 First report of a novel polymorphism and genetic characteristics of the leporine prion protein (PRNP) gene Kim, Dong-Ju Kim, Yong-Chan Jeong, Byung-Hoon Front Vet Sci Veterinary Science Transmissible spongiform encephalopathies (TSEs) have been reported in a broad spectrum of hosts. The genetic polymorphisms and characteristics of the prion protein (PRNP) gene have a vital impact on the development of TSEs. Notably, natural TSE infection cases have never been reported in rabbits, and genetic variations of the leporine PRNP gene have not been investigated to date. To identify leporine PRNP gene polymorphism, we performed amplicon sequencing in 203 rabbits. We report a novel single nucleotide polymorphism on the leporine PRNP gene. In addition, we performed a comparative analysis of amino acid sequences of prion protein (PrP) across several hosts using ClustalW2. Furthermore, we evaluated the effect of changes of unique leporine PrP amino acids with those conserved among various species using Swiss-Pdb Viewer. Interestingly, we found seven unique leporine amino acids, and the change of unique leporine amino acids with those conserved among other species, including S175N, Q221K, Q221R, A226Y, A230G, and A230S, was predicted to reduce hydrogen bonds in leporine PrP. Frontiers Media S.A. 2023-09-22 /pmc/articles/PMC10556520/ /pubmed/37808111 http://dx.doi.org/10.3389/fvets.2023.1229369 Text en Copyright © 2023 Kim, Kim and Jeong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Kim, Dong-Ju
Kim, Yong-Chan
Jeong, Byung-Hoon
First report of a novel polymorphism and genetic characteristics of the leporine prion protein (PRNP) gene
title First report of a novel polymorphism and genetic characteristics of the leporine prion protein (PRNP) gene
title_full First report of a novel polymorphism and genetic characteristics of the leporine prion protein (PRNP) gene
title_fullStr First report of a novel polymorphism and genetic characteristics of the leporine prion protein (PRNP) gene
title_full_unstemmed First report of a novel polymorphism and genetic characteristics of the leporine prion protein (PRNP) gene
title_short First report of a novel polymorphism and genetic characteristics of the leporine prion protein (PRNP) gene
title_sort first report of a novel polymorphism and genetic characteristics of the leporine prion protein (prnp) gene
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556520/
https://www.ncbi.nlm.nih.gov/pubmed/37808111
http://dx.doi.org/10.3389/fvets.2023.1229369
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