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First report of a novel polymorphism and genetic characteristics of the leporine prion protein (PRNP) gene
Transmissible spongiform encephalopathies (TSEs) have been reported in a broad spectrum of hosts. The genetic polymorphisms and characteristics of the prion protein (PRNP) gene have a vital impact on the development of TSEs. Notably, natural TSE infection cases have never been reported in rabbits, a...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556520/ https://www.ncbi.nlm.nih.gov/pubmed/37808111 http://dx.doi.org/10.3389/fvets.2023.1229369 |
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author | Kim, Dong-Ju Kim, Yong-Chan Jeong, Byung-Hoon |
author_facet | Kim, Dong-Ju Kim, Yong-Chan Jeong, Byung-Hoon |
author_sort | Kim, Dong-Ju |
collection | PubMed |
description | Transmissible spongiform encephalopathies (TSEs) have been reported in a broad spectrum of hosts. The genetic polymorphisms and characteristics of the prion protein (PRNP) gene have a vital impact on the development of TSEs. Notably, natural TSE infection cases have never been reported in rabbits, and genetic variations of the leporine PRNP gene have not been investigated to date. To identify leporine PRNP gene polymorphism, we performed amplicon sequencing in 203 rabbits. We report a novel single nucleotide polymorphism on the leporine PRNP gene. In addition, we performed a comparative analysis of amino acid sequences of prion protein (PrP) across several hosts using ClustalW2. Furthermore, we evaluated the effect of changes of unique leporine PrP amino acids with those conserved among various species using Swiss-Pdb Viewer. Interestingly, we found seven unique leporine amino acids, and the change of unique leporine amino acids with those conserved among other species, including S175N, Q221K, Q221R, A226Y, A230G, and A230S, was predicted to reduce hydrogen bonds in leporine PrP. |
format | Online Article Text |
id | pubmed-10556520 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-105565202023-10-07 First report of a novel polymorphism and genetic characteristics of the leporine prion protein (PRNP) gene Kim, Dong-Ju Kim, Yong-Chan Jeong, Byung-Hoon Front Vet Sci Veterinary Science Transmissible spongiform encephalopathies (TSEs) have been reported in a broad spectrum of hosts. The genetic polymorphisms and characteristics of the prion protein (PRNP) gene have a vital impact on the development of TSEs. Notably, natural TSE infection cases have never been reported in rabbits, and genetic variations of the leporine PRNP gene have not been investigated to date. To identify leporine PRNP gene polymorphism, we performed amplicon sequencing in 203 rabbits. We report a novel single nucleotide polymorphism on the leporine PRNP gene. In addition, we performed a comparative analysis of amino acid sequences of prion protein (PrP) across several hosts using ClustalW2. Furthermore, we evaluated the effect of changes of unique leporine PrP amino acids with those conserved among various species using Swiss-Pdb Viewer. Interestingly, we found seven unique leporine amino acids, and the change of unique leporine amino acids with those conserved among other species, including S175N, Q221K, Q221R, A226Y, A230G, and A230S, was predicted to reduce hydrogen bonds in leporine PrP. Frontiers Media S.A. 2023-09-22 /pmc/articles/PMC10556520/ /pubmed/37808111 http://dx.doi.org/10.3389/fvets.2023.1229369 Text en Copyright © 2023 Kim, Kim and Jeong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Kim, Dong-Ju Kim, Yong-Chan Jeong, Byung-Hoon First report of a novel polymorphism and genetic characteristics of the leporine prion protein (PRNP) gene |
title | First report of a novel polymorphism and genetic characteristics of the leporine prion protein (PRNP) gene |
title_full | First report of a novel polymorphism and genetic characteristics of the leporine prion protein (PRNP) gene |
title_fullStr | First report of a novel polymorphism and genetic characteristics of the leporine prion protein (PRNP) gene |
title_full_unstemmed | First report of a novel polymorphism and genetic characteristics of the leporine prion protein (PRNP) gene |
title_short | First report of a novel polymorphism and genetic characteristics of the leporine prion protein (PRNP) gene |
title_sort | first report of a novel polymorphism and genetic characteristics of the leporine prion protein (prnp) gene |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556520/ https://www.ncbi.nlm.nih.gov/pubmed/37808111 http://dx.doi.org/10.3389/fvets.2023.1229369 |
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