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Comparison of two Phaeodactylum tricornutum ecotypes under nitrogen starvation and resupply reveals distinct lipid accumulation strategies but a common degradation process
INTRODUCTION: Phaeodactylum tricornutum is a model species frequently used to study lipid metabolism in diatoms. When exposed to a nutrient limitation or starvation, diatoms are known to accumulate neutral lipids in cytoplasmic lipid droplets (LDs). Those lipids are produced partly de novo and partl...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556672/ https://www.ncbi.nlm.nih.gov/pubmed/37810403 http://dx.doi.org/10.3389/fpls.2023.1257500 |
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author | Murison, Victor Hérault, Josiane Côme, Martine Guinio, Sabrina Lebon, Alexis Chamot, Christophe Bénard, Magalie Galas, Ludovic Schoefs, Benoît Marchand, Justine Bardor, Muriel Ulmann, Lionel |
author_facet | Murison, Victor Hérault, Josiane Côme, Martine Guinio, Sabrina Lebon, Alexis Chamot, Christophe Bénard, Magalie Galas, Ludovic Schoefs, Benoît Marchand, Justine Bardor, Muriel Ulmann, Lionel |
author_sort | Murison, Victor |
collection | PubMed |
description | INTRODUCTION: Phaeodactylum tricornutum is a model species frequently used to study lipid metabolism in diatoms. When exposed to a nutrient limitation or starvation, diatoms are known to accumulate neutral lipids in cytoplasmic lipid droplets (LDs). Those lipids are produced partly de novo and partly from the recycle of plastid membrane lipids. Under a nitrogen resupply, the accumulated lipids are catabolized, a phenomenon about which only a few data are available. Various strains of P. tricornutum have been isolated around the world that may differ in lipid accumulation patterns. METHODS: To get further information on this topic, two genetically distant ecotypes of P. tricornutum (Pt1 and Pt4) have been cultivated under nitrogen deprivation during 11 days followed by a resupply period of 3 days. The importance of cytoplasmic LDs relative to the plastid was assessed by a combination of confocal laser scanning microscopy and cell volume estimation using bright field microscopy pictures. RESULTS AND DISCUSSION: We observed that in addition to a basal population of small LDs (0.005 μm(3) to 0.7 μm(3)) present in both strains all along the experiment, Pt4 cells immediately produced two large LDs (up to 12 μm(3) after 11 days) while Pt1 cells progressively produced a higher number of smaller LDs (up to 7 μm(3) after 11 days). In this work we showed that, in addition to intracellular available space, lipid accumulation may be limited by the pre-starvation size of the plastid as a source of membrane lipids to be recycled. After resupplying nitrogen and for both ecotypes, a fragmentation of the largest LDs was observed as well as a possible migration of LDs to the vacuoles that would suggest an autophagic degradation. Altogether, our results deepen the understanding of LDs dynamics and open research avenues for a better knowledge of lipid degradation in diatoms. |
format | Online Article Text |
id | pubmed-10556672 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-105566722023-10-07 Comparison of two Phaeodactylum tricornutum ecotypes under nitrogen starvation and resupply reveals distinct lipid accumulation strategies but a common degradation process Murison, Victor Hérault, Josiane Côme, Martine Guinio, Sabrina Lebon, Alexis Chamot, Christophe Bénard, Magalie Galas, Ludovic Schoefs, Benoît Marchand, Justine Bardor, Muriel Ulmann, Lionel Front Plant Sci Plant Science INTRODUCTION: Phaeodactylum tricornutum is a model species frequently used to study lipid metabolism in diatoms. When exposed to a nutrient limitation or starvation, diatoms are known to accumulate neutral lipids in cytoplasmic lipid droplets (LDs). Those lipids are produced partly de novo and partly from the recycle of plastid membrane lipids. Under a nitrogen resupply, the accumulated lipids are catabolized, a phenomenon about which only a few data are available. Various strains of P. tricornutum have been isolated around the world that may differ in lipid accumulation patterns. METHODS: To get further information on this topic, two genetically distant ecotypes of P. tricornutum (Pt1 and Pt4) have been cultivated under nitrogen deprivation during 11 days followed by a resupply period of 3 days. The importance of cytoplasmic LDs relative to the plastid was assessed by a combination of confocal laser scanning microscopy and cell volume estimation using bright field microscopy pictures. RESULTS AND DISCUSSION: We observed that in addition to a basal population of small LDs (0.005 μm(3) to 0.7 μm(3)) present in both strains all along the experiment, Pt4 cells immediately produced two large LDs (up to 12 μm(3) after 11 days) while Pt1 cells progressively produced a higher number of smaller LDs (up to 7 μm(3) after 11 days). In this work we showed that, in addition to intracellular available space, lipid accumulation may be limited by the pre-starvation size of the plastid as a source of membrane lipids to be recycled. After resupplying nitrogen and for both ecotypes, a fragmentation of the largest LDs was observed as well as a possible migration of LDs to the vacuoles that would suggest an autophagic degradation. Altogether, our results deepen the understanding of LDs dynamics and open research avenues for a better knowledge of lipid degradation in diatoms. Frontiers Media S.A. 2023-09-22 /pmc/articles/PMC10556672/ /pubmed/37810403 http://dx.doi.org/10.3389/fpls.2023.1257500 Text en Copyright © 2023 Murison, Hérault, Côme, Guinio, Lebon, Chamot, Bénard, Galas, Schoefs, Marchand, Bardor and Ulmann https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Murison, Victor Hérault, Josiane Côme, Martine Guinio, Sabrina Lebon, Alexis Chamot, Christophe Bénard, Magalie Galas, Ludovic Schoefs, Benoît Marchand, Justine Bardor, Muriel Ulmann, Lionel Comparison of two Phaeodactylum tricornutum ecotypes under nitrogen starvation and resupply reveals distinct lipid accumulation strategies but a common degradation process |
title | Comparison of two Phaeodactylum tricornutum ecotypes under nitrogen starvation and resupply reveals distinct lipid accumulation strategies but a common degradation process |
title_full | Comparison of two Phaeodactylum tricornutum ecotypes under nitrogen starvation and resupply reveals distinct lipid accumulation strategies but a common degradation process |
title_fullStr | Comparison of two Phaeodactylum tricornutum ecotypes under nitrogen starvation and resupply reveals distinct lipid accumulation strategies but a common degradation process |
title_full_unstemmed | Comparison of two Phaeodactylum tricornutum ecotypes under nitrogen starvation and resupply reveals distinct lipid accumulation strategies but a common degradation process |
title_short | Comparison of two Phaeodactylum tricornutum ecotypes under nitrogen starvation and resupply reveals distinct lipid accumulation strategies but a common degradation process |
title_sort | comparison of two phaeodactylum tricornutum ecotypes under nitrogen starvation and resupply reveals distinct lipid accumulation strategies but a common degradation process |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556672/ https://www.ncbi.nlm.nih.gov/pubmed/37810403 http://dx.doi.org/10.3389/fpls.2023.1257500 |
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