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Amplicon metagenomics of dung beetles (Coleoptera, Scarabaeidae, Scarabaeinae) as a proxy for lemur (Primates, Lemuroidea) studies in Madagascar
Dung beetles (Scarabaeidae, Scarabaeinae) are among the most cost-effective and informative biodiversity indicator groups, conveying rich information about the status of habitats and faunas of an area. Yet their use for monitoring the mammal species, that are the main providers of the food for the d...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Pensoft Publishers
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556875/ https://www.ncbi.nlm.nih.gov/pubmed/37810459 http://dx.doi.org/10.3897/zookeys.1181.107496 |
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author | Frolov, Andrey V. Akhmetova, Lilia A. Vishnevskaya, Maria S. Kiriukhin, Bogdan A. Montreuil, Olivier Lopes, Fernando Tarasov, Sergei I. |
author_facet | Frolov, Andrey V. Akhmetova, Lilia A. Vishnevskaya, Maria S. Kiriukhin, Bogdan A. Montreuil, Olivier Lopes, Fernando Tarasov, Sergei I. |
author_sort | Frolov, Andrey V. |
collection | PubMed |
description | Dung beetles (Scarabaeidae, Scarabaeinae) are among the most cost-effective and informative biodiversity indicator groups, conveying rich information about the status of habitats and faunas of an area. Yet their use for monitoring the mammal species, that are the main providers of the food for the dung beetles, has only recently been recognized. In the present work, we studied the diet of four endemic Madagascan dung beetles (Helictopleurusfissicollis (Fairmaire), H.giganteus (Harold), Nanosagaboides (Boucomont), and Epilissussplendidus Fairmaire) using high-throughput sequencing and amplicon metagenomics. For all beetle species, the ⅔–¾ of reads belonged to humans, suggesting that human feces are the main source of food for the beetles in the examined areas. The second most abundant were the reads of the cattle (Bostaurus Linnaeus). We also found lower but significant number of reads of six lemur species belonging to three genera. Our sampling localities agree well with the known ranges of these lemur species. The amplicon metagenomics method proved a promising tool for the lemur inventories in Madagascar. |
format | Online Article Text |
id | pubmed-10556875 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Pensoft Publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-105568752023-10-07 Amplicon metagenomics of dung beetles (Coleoptera, Scarabaeidae, Scarabaeinae) as a proxy for lemur (Primates, Lemuroidea) studies in Madagascar Frolov, Andrey V. Akhmetova, Lilia A. Vishnevskaya, Maria S. Kiriukhin, Bogdan A. Montreuil, Olivier Lopes, Fernando Tarasov, Sergei I. Zookeys Research Article Dung beetles (Scarabaeidae, Scarabaeinae) are among the most cost-effective and informative biodiversity indicator groups, conveying rich information about the status of habitats and faunas of an area. Yet their use for monitoring the mammal species, that are the main providers of the food for the dung beetles, has only recently been recognized. In the present work, we studied the diet of four endemic Madagascan dung beetles (Helictopleurusfissicollis (Fairmaire), H.giganteus (Harold), Nanosagaboides (Boucomont), and Epilissussplendidus Fairmaire) using high-throughput sequencing and amplicon metagenomics. For all beetle species, the ⅔–¾ of reads belonged to humans, suggesting that human feces are the main source of food for the beetles in the examined areas. The second most abundant were the reads of the cattle (Bostaurus Linnaeus). We also found lower but significant number of reads of six lemur species belonging to three genera. Our sampling localities agree well with the known ranges of these lemur species. The amplicon metagenomics method proved a promising tool for the lemur inventories in Madagascar. Pensoft Publishers 2023-09-28 /pmc/articles/PMC10556875/ /pubmed/37810459 http://dx.doi.org/10.3897/zookeys.1181.107496 Text en Andrey V. Frolov, Lilia A. Akhmetova, Maria S. Vishnevskaya, Bogdan A. Kiriukhin, Olivier Montreuil, Fernando Lopes, Sergei I. Tarasov https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Frolov, Andrey V. Akhmetova, Lilia A. Vishnevskaya, Maria S. Kiriukhin, Bogdan A. Montreuil, Olivier Lopes, Fernando Tarasov, Sergei I. Amplicon metagenomics of dung beetles (Coleoptera, Scarabaeidae, Scarabaeinae) as a proxy for lemur (Primates, Lemuroidea) studies in Madagascar |
title | Amplicon metagenomics of dung beetles (Coleoptera, Scarabaeidae, Scarabaeinae) as a proxy for lemur (Primates, Lemuroidea) studies in Madagascar |
title_full | Amplicon metagenomics of dung beetles (Coleoptera, Scarabaeidae, Scarabaeinae) as a proxy for lemur (Primates, Lemuroidea) studies in Madagascar |
title_fullStr | Amplicon metagenomics of dung beetles (Coleoptera, Scarabaeidae, Scarabaeinae) as a proxy for lemur (Primates, Lemuroidea) studies in Madagascar |
title_full_unstemmed | Amplicon metagenomics of dung beetles (Coleoptera, Scarabaeidae, Scarabaeinae) as a proxy for lemur (Primates, Lemuroidea) studies in Madagascar |
title_short | Amplicon metagenomics of dung beetles (Coleoptera, Scarabaeidae, Scarabaeinae) as a proxy for lemur (Primates, Lemuroidea) studies in Madagascar |
title_sort | amplicon metagenomics of dung beetles (coleoptera, scarabaeidae, scarabaeinae) as a proxy for lemur (primates, lemuroidea) studies in madagascar |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556875/ https://www.ncbi.nlm.nih.gov/pubmed/37810459 http://dx.doi.org/10.3897/zookeys.1181.107496 |
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