An easy and inexpensive method for determining the rate of individual phoretic events of nematodes

Considering their limited locomotory capabilities, the cosmopolitan distribution of free-living nematodes may rely on phoretic dispersal. We describe a new, inexpensive device to investigate individual phoretic events of the nematode Caenorhabditis elegans using the pomace flies Drosophila melanogaster and Drosophila hydei over short time periods. Using our device, we replicated previous findings demonstrating that phoresis requires C. elegans to be in the dauer stage and capable of nictation. Additionally, we find that phoresis can happen on the order of seconds, and does not increase linearly with time of interaction. Using this approach can facilitate the investigation of nematode biogeography, which could provide useful insight into their, and their vector’s, control.