Cargando…

The Antibacterial and Antifungal Activity of Chlorhexidine Diacetate Incorporated into Acrylic Resins Used in Provisional Restorations

OBJECTIVE: The surface of provisional restorations applied before conventional or implant- supported fixed restorations may cause bacterial or fungal biofilm formation. The aim of this study was to evaluate the antimicrobial activity of acrylic resins used in provisional restorations modified with c...

Descripción completa

Detalles Bibliográficos
Autores principales: Ata, Secil Ozkan, Akay, Canan, Mumcu, Emre, Erdonmez, Demet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: University of Zagreb School of Dental Medicine, and Croatian Dental Society - Croatian Medical Association 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10557115/
https://www.ncbi.nlm.nih.gov/pubmed/37808409
http://dx.doi.org/10.15644/asc57/3/4
_version_ 1785117015605772288
author Ata, Secil Ozkan
Akay, Canan
Mumcu, Emre
Erdonmez, Demet
author_facet Ata, Secil Ozkan
Akay, Canan
Mumcu, Emre
Erdonmez, Demet
author_sort Ata, Secil Ozkan
collection PubMed
description OBJECTIVE: The surface of provisional restorations applied before conventional or implant- supported fixed restorations may cause bacterial or fungal biofilm formation. The aim of this study was to evaluate the antimicrobial activity of acrylic resins used in provisional restorations modified with chlorhexidine diacetate. METHODS: 120 cylindrical, auto-polymerized resin samples modified with chlorhexidine diacetate were prepared at concentrations of 0 (control), 1, 3, 5 wt %. The antimicrobial activity was examined against Streptococcus mutans, Enterococcus faecalis, and Candida albicans using Crystal Violet quantification, MTT assay, and Scanning Electron Microscopy. Data were analyzed by ANOVA and paired sample t-tests (α=0.05). RESULTS: The addition of chlorhexidine diacetate influenced the growth rate and metabolic activity of microorganisms. The antimicrobial effect against C. albicans and S. mutans statistically increased with the percentage of chlorhexidine diacetate. E. faecalis bacteria were less affected by chlorhexidine diacetate compared to other pathogens. CONCLUSION: It has been shown that the effectiveness of CHDA in inhibiting the proliferation of microorganisms correlated positively with increasing concentration levels. More research is needed to confirm the impact of different chlorhexidine concentrations on the mechanical properties, clinical efficacy, and antimicrobial properties of CDHA.
format Online
Article
Text
id pubmed-10557115
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher University of Zagreb School of Dental Medicine, and Croatian Dental Society - Croatian Medical Association
record_format MEDLINE/PubMed
spelling pubmed-105571152023-10-07 The Antibacterial and Antifungal Activity of Chlorhexidine Diacetate Incorporated into Acrylic Resins Used in Provisional Restorations Ata, Secil Ozkan Akay, Canan Mumcu, Emre Erdonmez, Demet Acta Stomatol Croat Original Scientific Papers OBJECTIVE: The surface of provisional restorations applied before conventional or implant- supported fixed restorations may cause bacterial or fungal biofilm formation. The aim of this study was to evaluate the antimicrobial activity of acrylic resins used in provisional restorations modified with chlorhexidine diacetate. METHODS: 120 cylindrical, auto-polymerized resin samples modified with chlorhexidine diacetate were prepared at concentrations of 0 (control), 1, 3, 5 wt %. The antimicrobial activity was examined against Streptococcus mutans, Enterococcus faecalis, and Candida albicans using Crystal Violet quantification, MTT assay, and Scanning Electron Microscopy. Data were analyzed by ANOVA and paired sample t-tests (α=0.05). RESULTS: The addition of chlorhexidine diacetate influenced the growth rate and metabolic activity of microorganisms. The antimicrobial effect against C. albicans and S. mutans statistically increased with the percentage of chlorhexidine diacetate. E. faecalis bacteria were less affected by chlorhexidine diacetate compared to other pathogens. CONCLUSION: It has been shown that the effectiveness of CHDA in inhibiting the proliferation of microorganisms correlated positively with increasing concentration levels. More research is needed to confirm the impact of different chlorhexidine concentrations on the mechanical properties, clinical efficacy, and antimicrobial properties of CDHA. University of Zagreb School of Dental Medicine, and Croatian Dental Society - Croatian Medical Association 2023-09 /pmc/articles/PMC10557115/ /pubmed/37808409 http://dx.doi.org/10.15644/asc57/3/4 Text en University of Zagreb School of Dental Medicine https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (CC BY-NC-ND) 4.0 License.
spellingShingle Original Scientific Papers
Ata, Secil Ozkan
Akay, Canan
Mumcu, Emre
Erdonmez, Demet
The Antibacterial and Antifungal Activity of Chlorhexidine Diacetate Incorporated into Acrylic Resins Used in Provisional Restorations
title The Antibacterial and Antifungal Activity of Chlorhexidine Diacetate Incorporated into Acrylic Resins Used in Provisional Restorations
title_full The Antibacterial and Antifungal Activity of Chlorhexidine Diacetate Incorporated into Acrylic Resins Used in Provisional Restorations
title_fullStr The Antibacterial and Antifungal Activity of Chlorhexidine Diacetate Incorporated into Acrylic Resins Used in Provisional Restorations
title_full_unstemmed The Antibacterial and Antifungal Activity of Chlorhexidine Diacetate Incorporated into Acrylic Resins Used in Provisional Restorations
title_short The Antibacterial and Antifungal Activity of Chlorhexidine Diacetate Incorporated into Acrylic Resins Used in Provisional Restorations
title_sort antibacterial and antifungal activity of chlorhexidine diacetate incorporated into acrylic resins used in provisional restorations
topic Original Scientific Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10557115/
https://www.ncbi.nlm.nih.gov/pubmed/37808409
http://dx.doi.org/10.15644/asc57/3/4
work_keys_str_mv AT atasecilozkan theantibacterialandantifungalactivityofchlorhexidinediacetateincorporatedintoacrylicresinsusedinprovisionalrestorations
AT akaycanan theantibacterialandantifungalactivityofchlorhexidinediacetateincorporatedintoacrylicresinsusedinprovisionalrestorations
AT mumcuemre theantibacterialandantifungalactivityofchlorhexidinediacetateincorporatedintoacrylicresinsusedinprovisionalrestorations
AT erdonmezdemet theantibacterialandantifungalactivityofchlorhexidinediacetateincorporatedintoacrylicresinsusedinprovisionalrestorations
AT atasecilozkan antibacterialandantifungalactivityofchlorhexidinediacetateincorporatedintoacrylicresinsusedinprovisionalrestorations
AT akaycanan antibacterialandantifungalactivityofchlorhexidinediacetateincorporatedintoacrylicresinsusedinprovisionalrestorations
AT mumcuemre antibacterialandantifungalactivityofchlorhexidinediacetateincorporatedintoacrylicresinsusedinprovisionalrestorations
AT erdonmezdemet antibacterialandantifungalactivityofchlorhexidinediacetateincorporatedintoacrylicresinsusedinprovisionalrestorations