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Neural stem/progenitor cells from adult canine cervical spinal cord have the potential to differentiate into neural lineage cells
BACKGROUND: • Neural stem/progenitor cells (NSPCs) are multipotent self-renewing cells that can be isolated from the brain or spinal cord. As they need to be isolated from neural tissues, it is difficult to study human NSPCs. To facilitate NSPC research, we attempted to isolate NSPCs from dogs, as d...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10557334/ https://www.ncbi.nlm.nih.gov/pubmed/37803301 http://dx.doi.org/10.1186/s12917-023-03757-3 |
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author | Kim, Woo Keyoung Son, Yeon Sung Lim, Ji-Hey Kim, Wan Hee Kang, Byung-Jae |
author_facet | Kim, Woo Keyoung Son, Yeon Sung Lim, Ji-Hey Kim, Wan Hee Kang, Byung-Jae |
author_sort | Kim, Woo Keyoung |
collection | PubMed |
description | BACKGROUND: • Neural stem/progenitor cells (NSPCs) are multipotent self-renewing cells that can be isolated from the brain or spinal cord. As they need to be isolated from neural tissues, it is difficult to study human NSPCs. To facilitate NSPC research, we attempted to isolate NSPCs from dogs, as dogs share the environment and having many similar diseases with humans. We collected and established primary cultures of ependymal and subependymal cells from the central canal of the cervical spinal cord of adult dogs. To isolate pure NSPCs, we employed the monolayer culture and selective medium culture methods. We further tested the ability of the NSPCs to form neurospheres (using the suspension culture method) and evaluated their differentiation potential. RESULTS: • The cells had the ability to grow as cultures for up to 10 passages; the growth curves of the cells at the 3rd, 6th, and 9th passages showed similar patterns. The NSPCs were able to grow as neurospheres as well as monolayers, and immunostaining at the 3rd, 6th, and 9th passages showed that these cells expressed NSPC markers such as nestin and SOX2 (immunofluorescent staining). Monolayer cultures of NSPCs at the 3rd, 6th, and 9th passages were cultured for approximately 14 days using a differentiation medium and were observed to successfully differentiate into neural lineage and glial cells (astrocytes, neurons, and oligodendrocytes) at all the three passages tested. CONCLUSION: • It is feasible to isolate and propagate (up to at least 10 passages) canine cervical spinal cord-derived NSPCs with the capacity to differentiate into neuronal and glial cells. To the best of our knowledge this is the first study to successfully isolate, propagate, and differentiate canine NSPCs derived from cervical spinal cord in the adult canine, and we believe that these cells will contribute to the field of spinal cord regeneration in veterinary and comparative medicine. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-023-03757-3. |
format | Online Article Text |
id | pubmed-10557334 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-105573342023-10-07 Neural stem/progenitor cells from adult canine cervical spinal cord have the potential to differentiate into neural lineage cells Kim, Woo Keyoung Son, Yeon Sung Lim, Ji-Hey Kim, Wan Hee Kang, Byung-Jae BMC Vet Res Research BACKGROUND: • Neural stem/progenitor cells (NSPCs) are multipotent self-renewing cells that can be isolated from the brain or spinal cord. As they need to be isolated from neural tissues, it is difficult to study human NSPCs. To facilitate NSPC research, we attempted to isolate NSPCs from dogs, as dogs share the environment and having many similar diseases with humans. We collected and established primary cultures of ependymal and subependymal cells from the central canal of the cervical spinal cord of adult dogs. To isolate pure NSPCs, we employed the monolayer culture and selective medium culture methods. We further tested the ability of the NSPCs to form neurospheres (using the suspension culture method) and evaluated their differentiation potential. RESULTS: • The cells had the ability to grow as cultures for up to 10 passages; the growth curves of the cells at the 3rd, 6th, and 9th passages showed similar patterns. The NSPCs were able to grow as neurospheres as well as monolayers, and immunostaining at the 3rd, 6th, and 9th passages showed that these cells expressed NSPC markers such as nestin and SOX2 (immunofluorescent staining). Monolayer cultures of NSPCs at the 3rd, 6th, and 9th passages were cultured for approximately 14 days using a differentiation medium and were observed to successfully differentiate into neural lineage and glial cells (astrocytes, neurons, and oligodendrocytes) at all the three passages tested. CONCLUSION: • It is feasible to isolate and propagate (up to at least 10 passages) canine cervical spinal cord-derived NSPCs with the capacity to differentiate into neuronal and glial cells. To the best of our knowledge this is the first study to successfully isolate, propagate, and differentiate canine NSPCs derived from cervical spinal cord in the adult canine, and we believe that these cells will contribute to the field of spinal cord regeneration in veterinary and comparative medicine. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-023-03757-3. BioMed Central 2023-10-06 /pmc/articles/PMC10557334/ /pubmed/37803301 http://dx.doi.org/10.1186/s12917-023-03757-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Kim, Woo Keyoung Son, Yeon Sung Lim, Ji-Hey Kim, Wan Hee Kang, Byung-Jae Neural stem/progenitor cells from adult canine cervical spinal cord have the potential to differentiate into neural lineage cells |
title | Neural stem/progenitor cells from adult canine cervical spinal cord have the potential to differentiate into neural lineage cells |
title_full | Neural stem/progenitor cells from adult canine cervical spinal cord have the potential to differentiate into neural lineage cells |
title_fullStr | Neural stem/progenitor cells from adult canine cervical spinal cord have the potential to differentiate into neural lineage cells |
title_full_unstemmed | Neural stem/progenitor cells from adult canine cervical spinal cord have the potential to differentiate into neural lineage cells |
title_short | Neural stem/progenitor cells from adult canine cervical spinal cord have the potential to differentiate into neural lineage cells |
title_sort | neural stem/progenitor cells from adult canine cervical spinal cord have the potential to differentiate into neural lineage cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10557334/ https://www.ncbi.nlm.nih.gov/pubmed/37803301 http://dx.doi.org/10.1186/s12917-023-03757-3 |
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