HybriSeq: Probe-based Device-free Single-cell RNA Profiling

We have developed the HybriSeq method for single-cell RNA profiling, which utilizes in situ hybridization of multiple probes for targeted transcripts, followed by split-pool barcoding and sequencing analysis of the probes. We have shown that HybriSeq can achieve high sensitivity for RNA detection wi...

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Detalles Bibliográficos
Autores principales: Foyt, Daniel, Brown, David, Zhou, Shuqin, Huang, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10557710/
https://www.ncbi.nlm.nih.gov/pubmed/37808850
http://dx.doi.org/10.1101/2023.09.27.559406
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author Foyt, Daniel
Brown, David
Zhou, Shuqin
Huang, Bo
author_facet Foyt, Daniel
Brown, David
Zhou, Shuqin
Huang, Bo
author_sort Foyt, Daniel
collection PubMed
description We have developed the HybriSeq method for single-cell RNA profiling, which utilizes in situ hybridization of multiple probes for targeted transcripts, followed by split-pool barcoding and sequencing analysis of the probes. We have shown that HybriSeq can achieve high sensitivity for RNA detection with multiple probes and profile RNA accessibility. The utility of HybriSeq is demonstrated in characterizing cell-to-cell heterogeneities of a panel of 95 cell-cycle-related genes and the probe-probe heterogeneity within a single transcript.
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spelling pubmed-105577102023-10-07 HybriSeq: Probe-based Device-free Single-cell RNA Profiling Foyt, Daniel Brown, David Zhou, Shuqin Huang, Bo bioRxiv Article We have developed the HybriSeq method for single-cell RNA profiling, which utilizes in situ hybridization of multiple probes for targeted transcripts, followed by split-pool barcoding and sequencing analysis of the probes. We have shown that HybriSeq can achieve high sensitivity for RNA detection with multiple probes and profile RNA accessibility. The utility of HybriSeq is demonstrated in characterizing cell-to-cell heterogeneities of a panel of 95 cell-cycle-related genes and the probe-probe heterogeneity within a single transcript. Cold Spring Harbor Laboratory 2023-09-29 /pmc/articles/PMC10557710/ /pubmed/37808850 http://dx.doi.org/10.1101/2023.09.27.559406 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Foyt, Daniel
Brown, David
Zhou, Shuqin
Huang, Bo
HybriSeq: Probe-based Device-free Single-cell RNA Profiling
title HybriSeq: Probe-based Device-free Single-cell RNA Profiling
title_full HybriSeq: Probe-based Device-free Single-cell RNA Profiling
title_fullStr HybriSeq: Probe-based Device-free Single-cell RNA Profiling
title_full_unstemmed HybriSeq: Probe-based Device-free Single-cell RNA Profiling
title_short HybriSeq: Probe-based Device-free Single-cell RNA Profiling
title_sort hybriseq: probe-based device-free single-cell rna profiling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10557710/
https://www.ncbi.nlm.nih.gov/pubmed/37808850
http://dx.doi.org/10.1101/2023.09.27.559406
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