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Predicting cervical lymph node metastasis in OSCC based on computed tomography imaging genomics
BACKGROUND: To investigate the correlation between computed tomography (CT) radiomic characteristics and key genes for cervical lymph node metastasis (LNM) in oral squamous cell carcinoma (OSCC). METHODS: The region of interest was annotated at the edge of the primary tumor on enhanced CT images fro...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10557859/ https://www.ncbi.nlm.nih.gov/pubmed/37635388 http://dx.doi.org/10.1002/cam4.6474 |
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author | Jin, Nenghao Qiao, Bo Zhao, Min Li, Liangbo Zhu, Liang Zang, Xiaoyi Gu, Bin Zhang, Haizhong |
author_facet | Jin, Nenghao Qiao, Bo Zhao, Min Li, Liangbo Zhu, Liang Zang, Xiaoyi Gu, Bin Zhang, Haizhong |
author_sort | Jin, Nenghao |
collection | PubMed |
description | BACKGROUND: To investigate the correlation between computed tomography (CT) radiomic characteristics and key genes for cervical lymph node metastasis (LNM) in oral squamous cell carcinoma (OSCC). METHODS: The region of interest was annotated at the edge of the primary tumor on enhanced CT images from 140 patients with OSCC and obtained radiomic features. Ribonucleic acid (RNA) sequencing was performed on pathological sections from 20 patients. the DESeq software package was used to compare differential gene expression between groups. Weighted gene co‐expression network analysis was used to construct co‐expressed gene modules, and the KEGG and GO databases were used for pathway enrichment analysis of key gene modules. Finally, Pearson correlation coefficients were calculated between key genes of enriched pathways and radiomic features. RESULTS: Four hundred and eighty radiomic features were extracted from enhanced CT images of 140 patients; seven of these correlated significantly with cervical LNM in OSCC (p < 0.01). A total of 3527 differentially expressed RNAs were screened from RNA sequencing data of 20 cases. original_glrlm_RunVariance showed significant positive correlation with most long noncoding RNAs. CONCLUSIONS: OSCC cervical LNM is related to the salivary hair bump signaling pathway and biological process. Original_glrlm_RunVariance correlated with LNM and most differentially expressed long noncoding RNAs. |
format | Online Article Text |
id | pubmed-10557859 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-105578592023-10-07 Predicting cervical lymph node metastasis in OSCC based on computed tomography imaging genomics Jin, Nenghao Qiao, Bo Zhao, Min Li, Liangbo Zhu, Liang Zang, Xiaoyi Gu, Bin Zhang, Haizhong Cancer Med Research Articles BACKGROUND: To investigate the correlation between computed tomography (CT) radiomic characteristics and key genes for cervical lymph node metastasis (LNM) in oral squamous cell carcinoma (OSCC). METHODS: The region of interest was annotated at the edge of the primary tumor on enhanced CT images from 140 patients with OSCC and obtained radiomic features. Ribonucleic acid (RNA) sequencing was performed on pathological sections from 20 patients. the DESeq software package was used to compare differential gene expression between groups. Weighted gene co‐expression network analysis was used to construct co‐expressed gene modules, and the KEGG and GO databases were used for pathway enrichment analysis of key gene modules. Finally, Pearson correlation coefficients were calculated between key genes of enriched pathways and radiomic features. RESULTS: Four hundred and eighty radiomic features were extracted from enhanced CT images of 140 patients; seven of these correlated significantly with cervical LNM in OSCC (p < 0.01). A total of 3527 differentially expressed RNAs were screened from RNA sequencing data of 20 cases. original_glrlm_RunVariance showed significant positive correlation with most long noncoding RNAs. CONCLUSIONS: OSCC cervical LNM is related to the salivary hair bump signaling pathway and biological process. Original_glrlm_RunVariance correlated with LNM and most differentially expressed long noncoding RNAs. John Wiley and Sons Inc. 2023-08-27 /pmc/articles/PMC10557859/ /pubmed/37635388 http://dx.doi.org/10.1002/cam4.6474 Text en © 2023 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Jin, Nenghao Qiao, Bo Zhao, Min Li, Liangbo Zhu, Liang Zang, Xiaoyi Gu, Bin Zhang, Haizhong Predicting cervical lymph node metastasis in OSCC based on computed tomography imaging genomics |
title | Predicting cervical lymph node metastasis in OSCC based on computed tomography imaging genomics |
title_full | Predicting cervical lymph node metastasis in OSCC based on computed tomography imaging genomics |
title_fullStr | Predicting cervical lymph node metastasis in OSCC based on computed tomography imaging genomics |
title_full_unstemmed | Predicting cervical lymph node metastasis in OSCC based on computed tomography imaging genomics |
title_short | Predicting cervical lymph node metastasis in OSCC based on computed tomography imaging genomics |
title_sort | predicting cervical lymph node metastasis in oscc based on computed tomography imaging genomics |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10557859/ https://www.ncbi.nlm.nih.gov/pubmed/37635388 http://dx.doi.org/10.1002/cam4.6474 |
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