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Maize ZmHSP90 plays a role in acclimation to salt stress

BACKGROUND: Maize is sensitive to salt stress, especially during the germination and seedling stages. METHODS: We conducted germination experiments on 60 maize materials under salt stress, and screened out the most salt-tolerant and salt-sensitive varieties based on germination indicators. Afterward...

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Autores principales: Yu, Xinyan, Zhao, Yanxin, Wang, Yun, Zou, Zhiqing, Li, Fenghai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10557940/
https://www.ncbi.nlm.nih.gov/pubmed/37810777
http://dx.doi.org/10.7717/peerj.15819
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author Yu, Xinyan
Zhao, Yanxin
Wang, Yun
Zou, Zhiqing
Li, Fenghai
author_facet Yu, Xinyan
Zhao, Yanxin
Wang, Yun
Zou, Zhiqing
Li, Fenghai
author_sort Yu, Xinyan
collection PubMed
description BACKGROUND: Maize is sensitive to salt stress, especially during the germination and seedling stages. METHODS: We conducted germination experiments on 60 maize materials under salt stress, and screened out the most salt-tolerant and salt-sensitive varieties based on germination indicators. Afterwards, transcriptome analysis was performed to screen for key regulators in the plumule and flag leaf at the germination and seedling stages, respectively. Following that, transgenic tobacco was developed to expose the roles and mechanisms of the candidate genes, enabling a deeper investigation of their functions. RESULTS: Out of the 60 inbred lines of maize, “975-12” exhibits the highest level of salt tolerance, while “GEMS64” displays the lowest. The application of salt stress resulted in a significant increase in the levels of antioxidant enzymes in both “975-12” and “GEMS64”. ABA signal transduction and jasmonic acid pathways were the pathways that mainly affected by salt stress. In addition, a significant finding has been made indicating that when exposed to high levels of salt stress, the expression of ZmHSP90 in ‘975-12’ increased while in ‘GEMS64’ decreased. Furthermore, in tobacco plants overexpressing ZmHSP90, there was an increase in antioxidant enzyme activity associated with salt tolerance. ZmHSP90 enhanced the expression levels of NtSOS1, NtHKT1, and NtNHX1 as compared to those in the salt treatment, causing the maintenance of Na(+) and K(+) homeostasis, suggesting that high expression of ZmHSP90 was conducive to regulate Na(+) transporters to maintain K(+)/Na(+) balanced in tobacco.
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spelling pubmed-105579402023-10-07 Maize ZmHSP90 plays a role in acclimation to salt stress Yu, Xinyan Zhao, Yanxin Wang, Yun Zou, Zhiqing Li, Fenghai PeerJ Agricultural Science BACKGROUND: Maize is sensitive to salt stress, especially during the germination and seedling stages. METHODS: We conducted germination experiments on 60 maize materials under salt stress, and screened out the most salt-tolerant and salt-sensitive varieties based on germination indicators. Afterwards, transcriptome analysis was performed to screen for key regulators in the plumule and flag leaf at the germination and seedling stages, respectively. Following that, transgenic tobacco was developed to expose the roles and mechanisms of the candidate genes, enabling a deeper investigation of their functions. RESULTS: Out of the 60 inbred lines of maize, “975-12” exhibits the highest level of salt tolerance, while “GEMS64” displays the lowest. The application of salt stress resulted in a significant increase in the levels of antioxidant enzymes in both “975-12” and “GEMS64”. ABA signal transduction and jasmonic acid pathways were the pathways that mainly affected by salt stress. In addition, a significant finding has been made indicating that when exposed to high levels of salt stress, the expression of ZmHSP90 in ‘975-12’ increased while in ‘GEMS64’ decreased. Furthermore, in tobacco plants overexpressing ZmHSP90, there was an increase in antioxidant enzyme activity associated with salt tolerance. ZmHSP90 enhanced the expression levels of NtSOS1, NtHKT1, and NtNHX1 as compared to those in the salt treatment, causing the maintenance of Na(+) and K(+) homeostasis, suggesting that high expression of ZmHSP90 was conducive to regulate Na(+) transporters to maintain K(+)/Na(+) balanced in tobacco. PeerJ Inc. 2023-10-03 /pmc/articles/PMC10557940/ /pubmed/37810777 http://dx.doi.org/10.7717/peerj.15819 Text en ©2023 Yu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Yu, Xinyan
Zhao, Yanxin
Wang, Yun
Zou, Zhiqing
Li, Fenghai
Maize ZmHSP90 plays a role in acclimation to salt stress
title Maize ZmHSP90 plays a role in acclimation to salt stress
title_full Maize ZmHSP90 plays a role in acclimation to salt stress
title_fullStr Maize ZmHSP90 plays a role in acclimation to salt stress
title_full_unstemmed Maize ZmHSP90 plays a role in acclimation to salt stress
title_short Maize ZmHSP90 plays a role in acclimation to salt stress
title_sort maize zmhsp90 plays a role in acclimation to salt stress
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10557940/
https://www.ncbi.nlm.nih.gov/pubmed/37810777
http://dx.doi.org/10.7717/peerj.15819
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