Cargando…

Genome-wide analysis of the U-box E3 ubiquitin ligase family role in drought tolerance in sesame (Sesamum indicum L.)

Plant U-box (PUB) proteins belong to a class of ubiquitin ligases essential in various biological processes. Sesame (Sesamum indicum L.) is an important and worldwide cultivated oilseed crop. However few studies have been conducted to explore the role of PUBs in drought tolerance in sesame. This stu...

Descripción completa

Detalles Bibliográficos
Autores principales: Cao, Hengchun, Tian, Qiuzhen, Ju, Ming, Duan, Yinghui, Li, Guiting, Ma, Qin, Zhang, Haiyang, Zhang, Xianmei, Miao, Hongmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10558006/
https://www.ncbi.nlm.nih.gov/pubmed/37810391
http://dx.doi.org/10.3389/fpls.2023.1261238
_version_ 1785117193474670592
author Cao, Hengchun
Tian, Qiuzhen
Ju, Ming
Duan, Yinghui
Li, Guiting
Ma, Qin
Zhang, Haiyang
Zhang, Xianmei
Miao, Hongmei
author_facet Cao, Hengchun
Tian, Qiuzhen
Ju, Ming
Duan, Yinghui
Li, Guiting
Ma, Qin
Zhang, Haiyang
Zhang, Xianmei
Miao, Hongmei
author_sort Cao, Hengchun
collection PubMed
description Plant U-box (PUB) proteins belong to a class of ubiquitin ligases essential in various biological processes. Sesame (Sesamum indicum L.) is an important and worldwide cultivated oilseed crop. However few studies have been conducted to explore the role of PUBs in drought tolerance in sesame. This study identified a total of 56 members of the sesame PUB family (SiPUB) genes distributed unevenly across all 13 chromosomes. Based on phylogenetic analysis, all 56 SiPUB genes were classified into six groups with various structures and motifs. Cis-acting element analysis suggested that the SiPUB genes are involved in response to various stresses including drought. Based on RNA-seq analysis and quantitative real-time PCR, we identified nine SiPUB genes with significantly different expression profiles under drought stress. The expression patterns of six SiPUB genes in root, leaf and stem tissues corroborated the reliability of the RNA-seq datasets. These findings underscore the importance of SiPUB genes in enhancing drought tolerance in sesame plants. Our study provides novel insights into the evolutionary patterns and variations of PUB genes in sesame and lays the foundation for comprehending the functional characteristics of SiPUB genes under drought-induced stress conditions.
format Online
Article
Text
id pubmed-10558006
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-105580062023-10-07 Genome-wide analysis of the U-box E3 ubiquitin ligase family role in drought tolerance in sesame (Sesamum indicum L.) Cao, Hengchun Tian, Qiuzhen Ju, Ming Duan, Yinghui Li, Guiting Ma, Qin Zhang, Haiyang Zhang, Xianmei Miao, Hongmei Front Plant Sci Plant Science Plant U-box (PUB) proteins belong to a class of ubiquitin ligases essential in various biological processes. Sesame (Sesamum indicum L.) is an important and worldwide cultivated oilseed crop. However few studies have been conducted to explore the role of PUBs in drought tolerance in sesame. This study identified a total of 56 members of the sesame PUB family (SiPUB) genes distributed unevenly across all 13 chromosomes. Based on phylogenetic analysis, all 56 SiPUB genes were classified into six groups with various structures and motifs. Cis-acting element analysis suggested that the SiPUB genes are involved in response to various stresses including drought. Based on RNA-seq analysis and quantitative real-time PCR, we identified nine SiPUB genes with significantly different expression profiles under drought stress. The expression patterns of six SiPUB genes in root, leaf and stem tissues corroborated the reliability of the RNA-seq datasets. These findings underscore the importance of SiPUB genes in enhancing drought tolerance in sesame plants. Our study provides novel insights into the evolutionary patterns and variations of PUB genes in sesame and lays the foundation for comprehending the functional characteristics of SiPUB genes under drought-induced stress conditions. Frontiers Media S.A. 2023-09-19 /pmc/articles/PMC10558006/ /pubmed/37810391 http://dx.doi.org/10.3389/fpls.2023.1261238 Text en Copyright © 2023 Cao, Tian, Ju, Duan, Li, Ma, Zhang, Zhang and Miao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Cao, Hengchun
Tian, Qiuzhen
Ju, Ming
Duan, Yinghui
Li, Guiting
Ma, Qin
Zhang, Haiyang
Zhang, Xianmei
Miao, Hongmei
Genome-wide analysis of the U-box E3 ubiquitin ligase family role in drought tolerance in sesame (Sesamum indicum L.)
title Genome-wide analysis of the U-box E3 ubiquitin ligase family role in drought tolerance in sesame (Sesamum indicum L.)
title_full Genome-wide analysis of the U-box E3 ubiquitin ligase family role in drought tolerance in sesame (Sesamum indicum L.)
title_fullStr Genome-wide analysis of the U-box E3 ubiquitin ligase family role in drought tolerance in sesame (Sesamum indicum L.)
title_full_unstemmed Genome-wide analysis of the U-box E3 ubiquitin ligase family role in drought tolerance in sesame (Sesamum indicum L.)
title_short Genome-wide analysis of the U-box E3 ubiquitin ligase family role in drought tolerance in sesame (Sesamum indicum L.)
title_sort genome-wide analysis of the u-box e3 ubiquitin ligase family role in drought tolerance in sesame (sesamum indicum l.)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10558006/
https://www.ncbi.nlm.nih.gov/pubmed/37810391
http://dx.doi.org/10.3389/fpls.2023.1261238
work_keys_str_mv AT caohengchun genomewideanalysisoftheuboxe3ubiquitinligasefamilyroleindroughttoleranceinsesamesesamumindicuml
AT tianqiuzhen genomewideanalysisoftheuboxe3ubiquitinligasefamilyroleindroughttoleranceinsesamesesamumindicuml
AT juming genomewideanalysisoftheuboxe3ubiquitinligasefamilyroleindroughttoleranceinsesamesesamumindicuml
AT duanyinghui genomewideanalysisoftheuboxe3ubiquitinligasefamilyroleindroughttoleranceinsesamesesamumindicuml
AT liguiting genomewideanalysisoftheuboxe3ubiquitinligasefamilyroleindroughttoleranceinsesamesesamumindicuml
AT maqin genomewideanalysisoftheuboxe3ubiquitinligasefamilyroleindroughttoleranceinsesamesesamumindicuml
AT zhanghaiyang genomewideanalysisoftheuboxe3ubiquitinligasefamilyroleindroughttoleranceinsesamesesamumindicuml
AT zhangxianmei genomewideanalysisoftheuboxe3ubiquitinligasefamilyroleindroughttoleranceinsesamesesamumindicuml
AT miaohongmei genomewideanalysisoftheuboxe3ubiquitinligasefamilyroleindroughttoleranceinsesamesesamumindicuml