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compleasm: a faster and more accurate reimplementation of BUSCO

MOTIVATION: Evaluating the gene completeness is critical to measuring the quality of a genome assembly. An incomplete assembly can lead to errors in gene predictions, annotation, and other downstream analyses. Benchmarking Universal Single-Copy Orthologs (BUSCO) is a widely used tool for assessing t...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Neng, Li, Heng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10558035/
https://www.ncbi.nlm.nih.gov/pubmed/37758247
http://dx.doi.org/10.1093/bioinformatics/btad595
Descripción
Sumario:MOTIVATION: Evaluating the gene completeness is critical to measuring the quality of a genome assembly. An incomplete assembly can lead to errors in gene predictions, annotation, and other downstream analyses. Benchmarking Universal Single-Copy Orthologs (BUSCO) is a widely used tool for assessing the completeness of genome assembly by testing the presence of a set of single-copy orthologs conserved across a wide range of taxa. However, BUSCO is slow particularly for large genome assemblies. It is cumbersome to apply BUSCO to a large number of assemblies. RESULTS: Here, we present compleasm, an efficient tool for assessing the completeness of genome assemblies. Compleasm utilizes the miniprot protein-to-genome aligner and the conserved orthologous genes from BUSCO. It is 14 times faster than BUSCO for human assemblies and reports a more accurate completeness of 99.6% than BUSCO’s 95.7%, which is in close agreement with the annotation completeness of 99.5% for T2T-CHM13. AVAILABILITY AND IMPLEMENTATION: https://github.com/huangnengCSU/compleasm.