Cargando…

Systematic functional interrogation of SARS-CoV-2 host factors using Perturb-seq

Genomic and proteomic screens have identified numerous host factors of SARS-CoV-2, but efficient delineation of their molecular roles during infection remains a challenge. Here we use Perturb-seq, combining genetic perturbations with a single-cell readout, to investigate how inactivation of host fac...

Descripción completa

Detalles Bibliográficos
Autores principales:	Sunshine, Sara, Puschnik, Andreas S., Replogle, Joseph M., Laurie, Matthew T., Liu, Jamin, Zha, Beth Shoshana, Nuñez, James K., Byrum, Janie R., McMorrow, Aidan H., Frieman, Matthew B., Winkler, Juliane, Qiu, Xiaojie, Rosenberg, Oren S., Leonetti, Manuel D., Ye, Chun Jimmie, Weissman, Jonathan S., DeRisi, Joseph L., Hein, Marco Y.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10558542/ https://www.ncbi.nlm.nih.gov/pubmed/37803001 http://dx.doi.org/10.1038/s41467-023-41788-4

Ejemplares similares

Genome-Wide Knockout Screen Identifies Human Sialomucin CD164 as an Essential Entry Factor for Lymphocytic Choriomeningitis Virus
por: Liu, Jamin, et al.
Publicado: (2022)

Persistence of Ambigrammatic Narnaviruses Requires Translation of the Reverse Open Reading Frame
por: Retallack, Hanna, et al.
Publicado: (2021)

Chemical Rescue of Malaria Parasites Lacking an Apicoplast Defines Organelle Function in Blood-Stage Plasmodium falciparum
por: Yeh, Ellen, et al.
Publicado: (2011)

SARS-CoV-2 Variant Exposures Elicit Antibody Responses With Differential Cross-Neutralization of Established and Emerging Strains Including Delta and Omicron
por: Laurie, Matthew T, et al.
Publicado: (2022)

SARS-CoV-2 variant exposures elicit antibody responses with differential cross-neutralization of established and emerging strains including Delta and Omicron
por: Laurie, Matthew T, et al.
Publicado: (2021)

VersaCount: customizable manual tally software for cell counting
por: Kim, Charles C, et al.
Publicado: (2010)

Draft Genome Sequence of Picalivirus D Recovered from San Francisco Wastewater
por: Greninger, Alexander L., et al.
Publicado: (2015)

Draft Genome Sequence of Tombunodavirus UC1
por: Greninger, Alexander L., et al.
Publicado: (2015)

Draft Genome Sequence of Laverivirus UC1, a Dicistrovirus-Like RNA Virus Featuring an Unusual Genome Organization
por: Greninger, Alexander L., et al.
Publicado: (2015)

Draft Genome Sequences of Ciliovirus and Brinovirus from San Francisco Wastewater
por: Greninger, Alexander L., et al.
Publicado: (2015)

Draft Genome Sequences of Leviviridae RNA Phages EC and MB Recovered from San Francisco Wastewater
por: Greninger, Alexander L., et al.
Publicado: (2015)

Draft Genome Sequences of Marine RNA Viruses SF-1, SF-2, and SF-3 Recovered from San Francisco Wastewater
por: Greninger, Alexander L., et al.
Publicado: (2015)

RNA-Seq analysis of splicing in Plasmodium falciparum uncovers new splice junctions, alternative splicing and splicing of antisense transcripts
por: Sorber, Katherine, et al.
Publicado: (2011)

HMMSplicer: A Tool for Efficient and Sensitive Discovery of Known and Novel Splice Junctions in RNA-Seq Data
por: Dimon, Michelle T., et al.
Publicado: (2010)

A Draft Genome of the Honey Bee Trypanosomatid Parasite Crithidia mellificae
por: Runckel, Charles, et al.
Publicado: (2014)

Genome Sequence of a Divergent Avian Metapneumovirus from a Monk Parakeet (Myiopsitta monachus)
por: Retallack, Hanna, et al.
Publicado: (2019)

A Bayesian Network Driven Approach to Model the Transcriptional Response to Nitric Oxide in Saccharomyces cerevisiae
por: Zhu, Jingchun, et al.
Publicado: (2006)

Plate-based transfection and culturing technique for genetic manipulation of Plasmodium falciparum
por: Caro, Florence, et al.
Publicado: (2012)

PRICE: Software for the Targeted Assembly of Components of (Meta) Genomic Sequence Data
por: Ruby, J. Graham, et al.
Publicado: (2013)

MinorityReport, software for generalized analysis of causal genetic variants
por: Horst, Jeremy A., et al.
Publicado: (2017)

Functional characterization of 5′ UTR cis-acting sequence elements that modulate translational efficiency in Plasmodium falciparum and humans
por: Garcia, Valentina E., et al.
Publicado: (2022)

Whole-genome analysis of mRNA decay in Plasmodium falciparum reveals a global lengthening of mRNA half-life during the intra-erythrocytic development cycle
por: Shock, Jennifer L, et al.
Publicado: (2007)

Improved methods for magnetic purification of malaria parasites and haemozoin
por: Kim, Charles C, et al.
Publicado: (2010)

Identification and Manipulation of the Molecular Determinants Influencing Poliovirus Recombination
por: Runckel, Charles, et al.
Publicado: (2013)

Genome-wide regulatory dynamics of translation in the Plasmodium falciparum asexual blood stages
por: Caro, Florence, et al.
Publicado: (2014)

Transcriptome-wide characterization of the eIF4A signature highlights plasticity in translation regulation
por: Rubio, Claudia A, et al.
Publicado: (2014)

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Transmission Dynamics and Immune Responses in a Household of Vaccinated Persons
por: Liu, Jamin, et al.
Publicado: (2022)

Interrogating the real /
por: Žižek, Slavoj
Publicado: (2006)

The Interrogation Mark
por: Brooke, Margaret C.
Publicado: (1925)

The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins
por: Ngo, Ashley M, et al.
Publicado: (2019)

Combinatorial mathematical modelling approaches to interrogate rear retraction dynamics in 3D cell migration
por: Hetmanski, Joseph H. R., et al.
Publicado: (2021)

Broad susceptibility of Candida auris strains to 8-hydroxyquinolines and mechanisms of resistance
por: Lohse, Matthew B., et al.
Publicado: (2023)

Profile Hidden Markov Models for the Detection of Viruses within Metagenomic Sequence Data
por: Skewes-Cox, Peter, et al.
Publicado: (2014)

Whole Exome Sequencing of Growing and Non-Growing Cutaneous Neurofibromas from a Single Patient with Neurofibromatosis Type 1
por: Faden, Daniel L., et al.
Publicado: (2017)

Avian keratin disorder of Alaska black-capped chickadees is associated with Poecivirus infection
por: Zylberberg, Maxine, et al.
Publicado: (2018)

The Plasmodium falciparum cytoplasmic translation apparatus: a promising therapeutic target not yet exploited by clinically approved anti-malarials
por: Sheridan, Christine Moore, et al.
Publicado: (2018)

Correction: Whole Exome Sequencing of Growing and Non-Growing Cutaneous Neurofibromas from a Single Patient with Neurofibromatosis Type 1
por: Faden, Daniel L., et al.
Publicado: (2017)

How duplicated transcription regulators can diversify to govern the expression of nonoverlapping sets of genes
por: Pérez, J. Christian, et al.
Publicado: (2014)

Machine Learning Models to Interrogate Proteome-wide Cysteine Ligandabilities
por: Liu, Ruibin, et al.
Publicado: (2023)

Interrogatives about neutrinos
por: Pontecorvo, B M
Publicado: (1972)

Cannot write session to /tmp/vufind_sessions/sess_ofnd17jquvp61rjuvmo7vn5jkg