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Effective Treatment of Knee Osteoarthritis Using a Nano‐Enabled Drug Acupuncture Technology in Mice

A nano‐enabled drug delivery acupuncture technology (nd‐Acu) is developed that is based on traditional acupuncture needles where the stainless‐steel surface is designed to deliver various payload molecules. To create the nd‐Acu platform, an electrochemistry procedure is used to attach methyl salicyl...

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Autores principales: Xu, Wenjie, Xiao, Yu, Zhao, Minzhi, Zhu, Jiahui, Wang, Yu, Wang, Wenbin, Wang, Peng, Meng, Huan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10558644/
https://www.ncbi.nlm.nih.gov/pubmed/37555294
http://dx.doi.org/10.1002/advs.202302586
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author Xu, Wenjie
Xiao, Yu
Zhao, Minzhi
Zhu, Jiahui
Wang, Yu
Wang, Wenbin
Wang, Peng
Meng, Huan
author_facet Xu, Wenjie
Xiao, Yu
Zhao, Minzhi
Zhu, Jiahui
Wang, Yu
Wang, Wenbin
Wang, Peng
Meng, Huan
author_sort Xu, Wenjie
collection PubMed
description A nano‐enabled drug delivery acupuncture technology (nd‐Acu) is developed that is based on traditional acupuncture needles where the stainless‐steel surface is designed to deliver various payload molecules. To create the nd‐Acu platform, an electrochemistry procedure is used to attach methyl salicylate‐modified cyclodextrin in which the sugar rings allow the encapsulation of structurally defined single or multiple payload molecules via an inclusion complexation process. Drug loading and release profile are first studied using fluorescent dyes abiotically and at intact animal level. nd‐Acu allows more efficient dye loading and time‐dependent release compared to pristine needles without cyclodextrin modification. Subsequently, a proof‐of‐principle efficacy study is conducted using the platform to load a local anesthetic, lidocaine, for the treatment of knee osteoarthritis (KOA) in mice. It is demonstrated that lidocaine‐laden nd‐Acu can effectively alleviate pain, reduce inflammation, and slow down KOA development biochemically and histologically. Hypothesis‐driven and proteomic approaches are utilized to investigate the working mechanisms of lidocaine nd‐Acu, indicating that the therapeutic outcome is attributed to the in vivo modulation of the HMGB1/TLR4 signaling pathway. The study also obtained preliminary evidence suggesting the involvement of mitochondria as well as small GTPase such as cdc42 during the treatment by lidocaine nd‐Acu.
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spelling pubmed-105586442023-10-08 Effective Treatment of Knee Osteoarthritis Using a Nano‐Enabled Drug Acupuncture Technology in Mice Xu, Wenjie Xiao, Yu Zhao, Minzhi Zhu, Jiahui Wang, Yu Wang, Wenbin Wang, Peng Meng, Huan Adv Sci (Weinh) Research Articles A nano‐enabled drug delivery acupuncture technology (nd‐Acu) is developed that is based on traditional acupuncture needles where the stainless‐steel surface is designed to deliver various payload molecules. To create the nd‐Acu platform, an electrochemistry procedure is used to attach methyl salicylate‐modified cyclodextrin in which the sugar rings allow the encapsulation of structurally defined single or multiple payload molecules via an inclusion complexation process. Drug loading and release profile are first studied using fluorescent dyes abiotically and at intact animal level. nd‐Acu allows more efficient dye loading and time‐dependent release compared to pristine needles without cyclodextrin modification. Subsequently, a proof‐of‐principle efficacy study is conducted using the platform to load a local anesthetic, lidocaine, for the treatment of knee osteoarthritis (KOA) in mice. It is demonstrated that lidocaine‐laden nd‐Acu can effectively alleviate pain, reduce inflammation, and slow down KOA development biochemically and histologically. Hypothesis‐driven and proteomic approaches are utilized to investigate the working mechanisms of lidocaine nd‐Acu, indicating that the therapeutic outcome is attributed to the in vivo modulation of the HMGB1/TLR4 signaling pathway. The study also obtained preliminary evidence suggesting the involvement of mitochondria as well as small GTPase such as cdc42 during the treatment by lidocaine nd‐Acu. John Wiley and Sons Inc. 2023-08-09 /pmc/articles/PMC10558644/ /pubmed/37555294 http://dx.doi.org/10.1002/advs.202302586 Text en © 2023 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Xu, Wenjie
Xiao, Yu
Zhao, Minzhi
Zhu, Jiahui
Wang, Yu
Wang, Wenbin
Wang, Peng
Meng, Huan
Effective Treatment of Knee Osteoarthritis Using a Nano‐Enabled Drug Acupuncture Technology in Mice
title Effective Treatment of Knee Osteoarthritis Using a Nano‐Enabled Drug Acupuncture Technology in Mice
title_full Effective Treatment of Knee Osteoarthritis Using a Nano‐Enabled Drug Acupuncture Technology in Mice
title_fullStr Effective Treatment of Knee Osteoarthritis Using a Nano‐Enabled Drug Acupuncture Technology in Mice
title_full_unstemmed Effective Treatment of Knee Osteoarthritis Using a Nano‐Enabled Drug Acupuncture Technology in Mice
title_short Effective Treatment of Knee Osteoarthritis Using a Nano‐Enabled Drug Acupuncture Technology in Mice
title_sort effective treatment of knee osteoarthritis using a nano‐enabled drug acupuncture technology in mice
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10558644/
https://www.ncbi.nlm.nih.gov/pubmed/37555294
http://dx.doi.org/10.1002/advs.202302586
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