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Antioxidant activity of glucosamine and its effects on ROS production, Nrf2, and O-GlcNAc expression in HMEC-1 cells

Glucosamine (GlcN) is the most used supplement for osteoarthritis treatment. In vitro studies have related GlcN to beneficial and detrimental effects on health. The aim of this study was to evaluate the effects of O-linked-N-acetylglucosaminylation (O-GlcNAc) on GlcN-induced ROS production and Nrf2...

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Autores principales: Fernández-Rojas, B., Gómez-Sierra, T., Medina-Campos, O.N., Hernández-Juárez, J., Hernández-Cruz, P.A., Gallegos-Velasco, I.B., Pérez-Cervera, Y., Pedraza-Chaverri, J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10558709/
https://www.ncbi.nlm.nih.gov/pubmed/37808439
http://dx.doi.org/10.1016/j.crtox.2023.100128
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author Fernández-Rojas, B.
Gómez-Sierra, T.
Medina-Campos, O.N.
Hernández-Juárez, J.
Hernández-Cruz, P.A.
Gallegos-Velasco, I.B.
Pérez-Cervera, Y.
Pedraza-Chaverri, J.
author_facet Fernández-Rojas, B.
Gómez-Sierra, T.
Medina-Campos, O.N.
Hernández-Juárez, J.
Hernández-Cruz, P.A.
Gallegos-Velasco, I.B.
Pérez-Cervera, Y.
Pedraza-Chaverri, J.
author_sort Fernández-Rojas, B.
collection PubMed
description Glucosamine (GlcN) is the most used supplement for osteoarthritis treatment. In vitro studies have related GlcN to beneficial and detrimental effects on health. The aim of this study was to evaluate the effects of O-linked-N-acetylglucosaminylation (O-GlcNAc) on GlcN-induced ROS production and Nrf2 expression in human dermal microvascular endothelial cells-1 (HMEC-1) and to evaluate the antioxidant capacity of GlcN compared to well-known antioxidants. For this, we evaluate the antioxidant capacity by in vitro assays. Besides, the GlcN (5–20 mM) effects on cell viability, reactive oxygen species (ROS) production, O-GlcNAc, and nuclear factor erythroid-2-related factor 2 (Nrf2) expression with and without the O-GlcNAc inhibitor OSMI-1 (10 μM) in HMEC-1 were evaluated. GlcN showed high inhibitory concentration (low scavenging activity) against superoxide (O(2)(•─), IC(20) = 47.67 mM), 2,2-diphenyl-1-picrylhydrazyl (DPPH(•), IC(50) = 21.32 mM), and hydroxyl (HO(•), IC(50) = 14.04 mM) radicals without scavenging activity against hydrogen peroxide (H(2)O(2)) and low antioxidant capacity determined by oxygen radical absorbance capacity (ORAC, 0.001 mM Trolox equivalent) and ferric reducing antioxidant power (FRAP, 0.046 mM Trolox equivalent). In cell culture, GlcN (20 mM) reduced cell viability up to 26 % and induced an increase in ROS production (up to 70 %), O-GlcNAc (4-fold-higher vs. control), and Nrf2 expression (56 %), which were prevented by OSMI-1. These data suggest an association between O-GlcNAc, ROS production, and Nrf2 expression in HMEC-1 cells stimulated with GlcN.
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spelling pubmed-105587092023-10-08 Antioxidant activity of glucosamine and its effects on ROS production, Nrf2, and O-GlcNAc expression in HMEC-1 cells Fernández-Rojas, B. Gómez-Sierra, T. Medina-Campos, O.N. Hernández-Juárez, J. Hernández-Cruz, P.A. Gallegos-Velasco, I.B. Pérez-Cervera, Y. Pedraza-Chaverri, J. Curr Res Toxicol Research Article Glucosamine (GlcN) is the most used supplement for osteoarthritis treatment. In vitro studies have related GlcN to beneficial and detrimental effects on health. The aim of this study was to evaluate the effects of O-linked-N-acetylglucosaminylation (O-GlcNAc) on GlcN-induced ROS production and Nrf2 expression in human dermal microvascular endothelial cells-1 (HMEC-1) and to evaluate the antioxidant capacity of GlcN compared to well-known antioxidants. For this, we evaluate the antioxidant capacity by in vitro assays. Besides, the GlcN (5–20 mM) effects on cell viability, reactive oxygen species (ROS) production, O-GlcNAc, and nuclear factor erythroid-2-related factor 2 (Nrf2) expression with and without the O-GlcNAc inhibitor OSMI-1 (10 μM) in HMEC-1 were evaluated. GlcN showed high inhibitory concentration (low scavenging activity) against superoxide (O(2)(•─), IC(20) = 47.67 mM), 2,2-diphenyl-1-picrylhydrazyl (DPPH(•), IC(50) = 21.32 mM), and hydroxyl (HO(•), IC(50) = 14.04 mM) radicals without scavenging activity against hydrogen peroxide (H(2)O(2)) and low antioxidant capacity determined by oxygen radical absorbance capacity (ORAC, 0.001 mM Trolox equivalent) and ferric reducing antioxidant power (FRAP, 0.046 mM Trolox equivalent). In cell culture, GlcN (20 mM) reduced cell viability up to 26 % and induced an increase in ROS production (up to 70 %), O-GlcNAc (4-fold-higher vs. control), and Nrf2 expression (56 %), which were prevented by OSMI-1. These data suggest an association between O-GlcNAc, ROS production, and Nrf2 expression in HMEC-1 cells stimulated with GlcN. Elsevier 2023-09-22 /pmc/articles/PMC10558709/ /pubmed/37808439 http://dx.doi.org/10.1016/j.crtox.2023.100128 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Fernández-Rojas, B.
Gómez-Sierra, T.
Medina-Campos, O.N.
Hernández-Juárez, J.
Hernández-Cruz, P.A.
Gallegos-Velasco, I.B.
Pérez-Cervera, Y.
Pedraza-Chaverri, J.
Antioxidant activity of glucosamine and its effects on ROS production, Nrf2, and O-GlcNAc expression in HMEC-1 cells
title Antioxidant activity of glucosamine and its effects on ROS production, Nrf2, and O-GlcNAc expression in HMEC-1 cells
title_full Antioxidant activity of glucosamine and its effects on ROS production, Nrf2, and O-GlcNAc expression in HMEC-1 cells
title_fullStr Antioxidant activity of glucosamine and its effects on ROS production, Nrf2, and O-GlcNAc expression in HMEC-1 cells
title_full_unstemmed Antioxidant activity of glucosamine and its effects on ROS production, Nrf2, and O-GlcNAc expression in HMEC-1 cells
title_short Antioxidant activity of glucosamine and its effects on ROS production, Nrf2, and O-GlcNAc expression in HMEC-1 cells
title_sort antioxidant activity of glucosamine and its effects on ros production, nrf2, and o-glcnac expression in hmec-1 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10558709/
https://www.ncbi.nlm.nih.gov/pubmed/37808439
http://dx.doi.org/10.1016/j.crtox.2023.100128
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